We narrowed to 6,078 results for: nls

1. pCDNA3.0_nucNanobody IRES EGFP

   Plasmid

   #136619

   Purpose

   Plasmid encoding nuclear localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   SNAP-Tag-EnhancerNb-NLS (IRES EGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3xFLAG-dCas9/pMXs-neo

   Plasmid

   #51260

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. epB_UbC_tdMS2cp-tagRFP-Tx2

   Plasmid

   #119908

   Purpose

   ePiggyBac mammalian expression plasmid for tandem dimer MS2cp-tagRFP-T fusion

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   HA-tdMS2cp-tagRFP-Tx2-nls
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMpGWB215

   Plasmid

   #68606

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Nuclear localization signal-tagged Citrine (Citri…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMZ-178

   Plasmid

   #195426

   Purpose

   Transient, constitutive expression of synZiFTR: ZF3-p65

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pUb - 3XFLAG - NLS - ZF3-5 (ZF3) - p65 - RbGpA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMZ-185

   Plasmid

   #195433

   Purpose

   Transient, constitutive expression of synZiFTR: ZF10-p65

   Depositor

   Ahmad Khalil

   Article

   Li et al Science. 2022 Dec 16;378(6625):1227-1234

   Insert

   pUb - 3XFLAG - NLS - ZF10-1 (ZF10) - p65 - RbGpA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSL2620 (pTniQ_Vch)

   Plasmid

   #200892

   Purpose

   Expresses human codon-optimized V. cholerae TniQ from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchTniQ
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBK-EQR-BE3

   Plasmid

   #85172

   Purpose

   Expresses EQR-BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   EQR-BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ154

   Plasmid

   #69305

   Purpose

   Gateway entry vector with AteCas9 (Arabidopsis codon optimized)

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   AteCas9 (Arabidopsis codon optimized)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   NLS fusion

   Promoter

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. M-ST1n-VP64

    Plasmid

    #48675

    Purpose

    Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Expression

    Mutation

    nuclease-null (D9A D598A H599A N622A)

    Promoter

    CMV

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pH-PABE-7-sgRNA

    Plasmid

    #115621

    Purpose

    Targeted A to G in rice

    Depositor

    Caixia Gao

    Article

    Li et al Genome Biol. 2018 May 29;19(1):59. doi:

    Insert

    wtTadA-TadA7.10-nCas9-3*NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 3xFLAG-dCas9/pMSCVneo

    Plasmid

    #134982

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 3xFLAG-dCas9/pMSCVpuro

    Plasmid

    #134983

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. vas-Cas9.874Z

    Plasmid

    #112687

    Purpose

    Express hSpCas9 under vasa promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    hSpCas9
    

    Use

    Tags

    3x FLAG, EGFP, and NLS

    Expression

    Insect

    Mutation

    Humanized Cas9 bacterial sequence

    Promoter

    vasa

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-REPORT(PGK)-12xHRE

    Plasmid

    #172334

    Purpose

    hypoxic responsive reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

    Plasmid

    #200910

    Purpose

    3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

    Depositor

    Debojyoti Chakraborty

    Insert

    3XHA_den1FnCas9_KRAB_eGFP
    

    Use

    CRISPR

    Tags

    3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP

    Expression

    Mammalian

    Mutation

    Changed Glutamic Acid to Arginine at 1369 position

    Promoter

    CBH

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3xFLAG-dCas9/pMXs-IG

    Plasmid

    #51258

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGLTR-X-PURO

    Plasmid

    #58246

    Purpose

    GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selection

    Depositor

    Stephan Geley

    Article

    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

    Insert

    Puro(R)
    

    Use

    Lentiviral and RNAi; Gateway destionation vector

    Tags

    TetR-NLS-T2A

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSL2621 (pCas8_Vch)

    Plasmid

    #200893

    Purpose

    Expresses human codon-optimized V. cholerae Cas8 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas8
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSL2623 (pCas6_Vch)

    Plasmid

    #200895

    Purpose

    Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas6
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge