We narrowed to 4,337 results for: Pet2

1. ACS_27

   Plasmid

   #111652

   Purpose

   Funclib design ACS_27

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

   Insert

   ACS_27
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTH861-gst-optec P210T

   Plasmid

   #89595

   Purpose

   Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
   

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   changed proline 210 to threonine

   Promoter

   T7

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTH860-gst-optec A180Q

   Plasmid

   #89594

   Purpose

   Plasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
   

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   changed alanine 180 to glutamine

   Promoter

   T7

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTH858-gst-optec V156E

   Plasmid

   #89592

   Purpose

   Plasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase V156E mutant, codon-optimised for bacterial expression
   

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   changed valine 156 to glutamic acid

   Promoter

   T7

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTH859-gst-optec Y172M

   Plasmid

   #89593

   Purpose

   Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
   

   Tags

   8xhistidine

   Expression

   Bacterial

   Mutation

   changed tyrosine 172 to methionine

   Promoter

   T7

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. CTL2 (M2225R)

   Plasmid

   #96952

   Purpose

   Expresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coli

   Depositor

   Douglas Rees

   Article

   Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

   Insert

   CTL2 (M222R) of PIEZO1
   

   Tags

   6x Histidine Tag

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRAF-MutL(R95F)

   Plasmid

   #86608

   Purpose

   E. coli Mutl(R95F) expression plasmid

   Depositor

   Richard Fishel

   Article

   Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

   Insert

   E. coli MutL(R95F)
   

   Tags

   his-tag and ald-tag

   Expression

   Bacterial

   Mutation

   R95F

   Promoter

   T7

   Available Since

   June 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK359

   Plasmid

   #71570

   Purpose

   Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase (UctA)

   Depositor

   T. J. Kappock

   Article

   Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

   Insert

   putative CoA-transferase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJK278

   Plasmid

   #71134

   Purpose

   Expression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changes histidine-89 to asparagine; additional N-…

   Promoter

   T7

   Available Since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK130

    Plasmid

    #71129

    Purpose

    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changed valine-319 to phenylalanine

    Promoter

    T7

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK591

    Plasmid

    #71589

    Purpose

    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala/Asp177>Ala mutants (UctC-T2A,D177A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:

    Insert

    acetyl-CoA:oxalate CoA-transferase
    

    Expression

    Bacterial

    Mutation

    changes threonine-2 to alanine and aspartate-177 …

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJK286

    Plasmid

    #71567

    Purpose

    Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (UctB)

    Depositor

    T. J. Kappock

    Article

    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

    Insert

    formyl-CoA:oxalate CoA-transferase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK358

    Plasmid

    #71569

    Purpose

    Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase (UctD)

    Depositor

    T. J. Kappock

    Article

    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

    Insert

    putative CoA-transferase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJK320

    Plasmid

    #71144

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59S mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes histidine-59 to serine; additional N-term…

    Promoter

    T7

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK319

    Plasmid

    #71143

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes histidine-59 to alanine; additional N-ter…

    Promoter

    T7

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK318

    Plasmid

    #71142

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes histidine-59 to glutamine; additional N-t…

    Promoter

    T7

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK315

    Plasmid

    #71141

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes histidine-89 to glycine; additional N-ter…

    Promoter

    T7

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK314

    Plasmid

    #71140

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes histidine-89 to valine; additional N-term…

    Promoter

    T7

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK174

    Plasmid

    #71835

    Purpose

    Expression of Acetobacter aceti 1023 PurE and PurK in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Expression

    Bacterial

    Mutation

    additional N-terminal methionine encoded by this …

    Promoter

    T7 and endogenous

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK279

    Plasmid

    #71135

    Purpose

    Expression of Acetobacter aceti 1023 PurE (H89D mutant) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes histidine-89 to aspartate; additional N-t…

    Promoter

    T7

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge