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We narrowed to 7,654 results for: Pol

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Showing: 3861 - 3880 of 7654 results

  1. G30, Cdc20-mCherry-AID tagging

    Plasmid
    #130270
    Purpose
    Tagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombination
    Depositor
    Matthias Heinemann
    Publication
    Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1
    Insert
    Cdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
    Tags
    AID(71-114) and mCherry
    Expression
    Yeast
    Promoter
    Native promoter
    Available since
    Sept. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    130270-plasmid-map-sequence-id-255828

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  3. GGE152

    Plasmid
    #120752
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_8UASxpr-TEF)
    Depositor
    Tristan Rossignol
    Publication
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    P2_8UASxpr-TEF
    Use
    Cloning/assembly plasmid
    Available since
    Dec. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    120752-plasmid-map-sequence-id-262896

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  4. GGE147

    Plasmid
    #120743
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_8UASxpr-TEF)
    Depositor
    Tristan Rossignol
    Publication
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    P1_8UASxpr-TEF
    Use
    Cloning/assembly plasmid
    Available since
    Dec. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    120743-plasmid-map-sequence-id-331631

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  5. GGE317

    Plasmid
    #120761
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 3 (P3_8UASxpr-TEF)
    Depositor
    Tristan Rossignol
    Publication
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    P3_8UASxpr-TEF
    Use
    Cloning/assembly plasmid
    Available since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    120761-plasmid-map-sequence-id-263096

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  6. GGE250

    Plasmid
    #120746
    Purpose
    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position1 (P1_EYK300-5AB)
    Depositor
    Tristan Rossignol
    Publication
    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.
    Insert
    P1_EYK300-5AB
    Use
    Cloning/assembly plasmid
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    120746-plasmid-map-sequence-id-259344

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  7. pST50Trc1-FLAGHISNDHFR

    Plasmid
    #63947
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Publication
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis & FLAG; TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63947-plasmid-map-sequence-id-177457

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  8. pST50Trc1-STRNDHFR

    Plasmid
    #63942
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Publication
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Strep II (STR); TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63942-plasmid-map-sequence-id-177348

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  9. pST50Trc1-CBPDHFR

    Plasmid
    #63936
    Purpose
    Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Publication
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    Calmodulin Binding Peptide (CBP)
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    63936-plasmid-map-sequence-id-177342

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  10. DPYSL3A-c010

    Plasmid
    #53641
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DPYSL3A
    Use
    Baculovirus transfer vector
    Tags
    His6-ZBasic-TEV
    Promoter
    polyhedrin promoter
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  15. pTBL3440 MTK_PBdest lacO-hU6-BtoApegRNA-LacI-mCherry

    Plasmid
    #254862
    Purpose
    peCHYRON destination vector expressing IPTG-inducible B-to-A pegRNA, LacI, and mCherry. Can be integrated into the genome of mammalian cells with PiggyBac transposase.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d
    Inserts
    lacO_hU6_promoter-peCHYRON_BtoA_pegRNA
    pCAG-LacI-rb_glob_polyA
    pEF1alpha-mCherry-BGH_polyA
    Use
    Piggybac integration
    Expression
    Mammalian
    Available since
    June 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    254862-plasmid-map-sequence-id-512990

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  16. pTBL3439 MTK_PBdest lacO-hU6-AtoBpegRNA-LacI-mCherry

    Plasmid
    #254861
    Purpose
    peCHYRON destination vector expressing IPTG-inducible A-to-B pegRNA, LacI, and mCherry. Can be integrated into the genome of mammalian cells with PiggyBac transposase.
    Depositor
    Chang Liu
    Publication
    Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d
    Inserts
    lacO_hU6_promoter-peCHYRON_AtoB_pegRNA
    pCAG-LacI-rb_glob_polyA
    pEF1alpha-mCherry-BGH_polyA
    Use
    Piggybac integration
    Expression
    Mammalian
    Available since
    June 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    254861-plasmid-map-sequence-id-512989

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  17. ATX3-77Q-R388G

    Plasmid
    #184251
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Publication
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Human, Synthetic)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    184251-plasmid-map-sequence-id-409179

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  18. pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)

    Plasmid
    #169181
    Purpose
    Baculoviral transfer vector to express untagged nsp7-Linker-nsp8 fusion protein (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Publication
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp7-Linker-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    Tags
    No purification tag and nsp7-nsp8 fusion protein …
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169181-plasmid-map-sequence-id-334012

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  20. pKI1

    Plasmid
    #199118
    Purpose
    Expresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labelling
    Depositor
    Jeff Gelles
    Publication
    Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12
    Insert
    H6-SNAP-RapA
    Tags
    6xHis and SNAP-tag
    Expression
    Bacterial
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    199118-plasmid-map-sequence-id-388190

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Showing: 3861 - 3880 of 7654 results