Skip to main content
Addgene

We narrowed to 5,563 results for: org

Showing: 3861 - 3880 of 5563 results
  1. pT2iU6Apowriter2N(#355)

    Plasmid
    #184106
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis
    Depositor
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transient transgenesis + in vitro trans…
    Tags
    internal tag: 5myc
    Expression
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi+SP6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. Human/mouse ELL2

    Plasmid
    #127254
    Purpose
    Expresses a human/mouse chimera that increases ELL2 expression in mammalian cells
    Depositor
    Insert
    N-terminal human/C-terminal mouse ELL2 (ELL2, Ell2 Human, Mouse)
    Use
    Other
    Tags
    His6X
    Expression
    Mammalian
    Mutation
    N terminal amino acids (1-287) are human, C termi…
    Promoter
    pEF1a
    Available since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pF1-Hs Cdk1/cyclin B1/Cks1

    Plasmid
    #177329
    Purpose
    Co-expression of the human active Cdk1/cyclin B1/Cks1 kinase complex
    Depositor
    Inserts
    Cyclin-dependent kinase 1 (CDK1 Human)
    G2/mitotic-specific cyclin-B1 (CCNB1 Human)
    Cyclin-dependent kinases regulatory subunit 1 (CKS1B Human)
    Use
    Tags
    2 x Strep-tag and 8 x His
    Expression
    Insect
    Mutation
    Promoter
    p10 and polh
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. pOPINB-M1-di-Ub(G76V)-HOIL-1

    Plasmid
    #229539
    Purpose
    Constitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coli
    Inserts
    Ubiquitin (UBC Human)
    Ubiquitin (UBC Human)
    HOIL-1 (RBCK1 Human)
    Use
    Tags
    6xHis-3C
    Expression
    Bacterial
    Mutation
    Changed Gly 76 to Val and changed Gly 76 to Val
    Promoter
    T7
    Available since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pTRIP TRE BI_SEPT2 NCmut-14-GFP11_SEPT2 NCmut-14-GFP10

    Plasmid
    #180357
    Purpose
    mammalian co-expression of human SEPT2 NCmut fused to GFP10 and of human SEPT2 NCmut fused to GFP11
    Depositor
    Inserts
    SEPTIN2 (SEPTIN2 Human)
    SEPTIN 2 (SEPTIN2 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT2 F20D, V27D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pTRIP TRE BI_SEPT9_i1 NCmut-14-GFP11_SEPT9_i1 NCmut-14-GFP10

    Plasmid
    #180358
    Purpose
    mammalian co-expression of human SEPT9_i1 NCmut fused to GFP10 and of human SEPT9_i1 NCmut fused to GFP11
    Depositor
    Inserts
    SEPTIN9_v1 (SEPTIN9 Human)
    SEPTIN9_v1 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT9_i1 I281D, M288D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid
    #224697
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Use
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. PGL4.11-COL1A1double mutants

    Plasmid
    #230918
    Purpose
    Double mutation of TEAD and SMAD binding elements in COL1A1 promoter; check the promoter activity.
    Depositor
    Insert
    Human CLO1A1 promoter including SMAD and TEAD mutant elements (COL1A1 Human)
    Use
    Luciferase
    Tags
    Luciferase-luc2p
    Expression
    Bacterial and Mammalian
    Mutation
    COL1A1 promoter with mutant SMAD and TEAD binding…
    Promoter
    COL1A1 with mutant SMAD and TEAD elements
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_GFP10-14-SEPT7 Gmut1

    Plasmid
    #180354
    Purpose
    mammalian co-expression of human SEPT7 Gmut1 fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11
    Depositor
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN7 (SEPTIN7 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT7 W269A, H279D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_SEPT9_i1 Gmut-14-GFP10

    Plasmid
    #180355
    Purpose
    mammalian co-expression of human SEPT9_i1 Gmut fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11
    Depositor
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN 9 (SEPTIN9 )
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT7 W269A, H279D and SEPT9_i1 W520A, H530D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. pTRIP TRE BI_GFP11-14-SEPT7 Gmut1_SEPT9_i3 Gmut-14-GFP10

    Plasmid
    #180356
    Purpose
    mammalian co-expression of human SEPT9_i3 Gmut fused to GFP10 and of human SEPT7 Gmut1 fused to GFP11
    Depositor
    Inserts
    SEPTIN7 (SEPTIN7 Human)
    SEPTIN 9 (SEPTIN9 )
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT7 W269A, H279D and SEPT9_i3 W502A, H512D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. pTRIP TRE BI_SEPT9_i3 NCmut-14-GFP11_SEPT9_i3 NCmut-14-GFP10

    Plasmid
    #180360
    Purpose
    mammalian co-expression of human SEPT9_i3 NCmut fused to GFP10 and of human SEPT9_i3 NCmut fused to GFP11
    Depositor
    Inserts
    SEPTIN9_v3 (SEPTIN9 Human)
    SEPTIN9_v3 (SEPTIN9 Human)
    IRES-TagBFP
    Use
    Lentiviral
    Tags
    GFP10 and GFP11
    Expression
    Mammalian
    Mutation
    SEPT9_i3 I263D, M270D
    Promoter
    bidirectional TRE
    Available since
    Dec. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. AAV-pCamKII-Cre-pU6-SpCas9gRNAentry

    Plasmid
    #210391
    Purpose
    AAV plasmid encoding the CamKII promoter driving Cre expression, along with SpCas9 gRNA entry cassette (RFP dropout)
    Insert
    AAV-(ITR)-pCamKII-NLS-Cre-WPRE-bGHpA-(rev)-SpCas9_gRNA-BsmBI_RFPcassette-hU6-(ITR) (NJA158)
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CamKII promoter for Cre, human U6 promoter for Sp…
    Available since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid
    #224698
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Use
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 3861 - 3880 of 5563 results