Addgene

We narrowed to 14,540 results for: pET

Showing: 1941 - 1950 of 14540 results

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pET21b-rrt-His8

Plasmid

#194088

Purpose

Expresses Ec86 reverse transcriptase in Escherichia coli

Depositor

Tingting Zou

Article

Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

Insert

Ec86 rrt

Use

Tags

His8

Expression

Mutation

Promoter

T7

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-GFP-mHP1alpha

Plasmid

#181912

Purpose

Bacterial protein expression vector for GFP-tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_09

Plasmid

#194670

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_09 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_09

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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VV001: WT Arch-3 in pET28b

Plasmid

#58487

Purpose

Expresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coli

Depositor

Adam Cohen

Article

Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Archaerhodopsin-3 (with C-terminal His tag)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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pET3d-E2-25K-C170S

Plasmid

#18892

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Insert

Ubiquitin conjugating enzyme E2-25K (UBE2K Most likely H. sapiens, Human)

Use

Tags

Expression

Bacterial

Mutation

Cysteine 170 is replaced with a Serine residue.

Promoter

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pET21-GFP-AURKA

Plasmid

#99875

Purpose

Expression of GFP-AURKA for protein purification in bacteria

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET51b-NAD-cpLuc2

Plasmid

#135629

Purpose

Bacterial expression of a biosensor for measuring NAD+ levels at the point of care (V2)

Depositor

Kai Johnsson

Article

Yu et al Nat Metab. 2019 Dec;1(12):1219-1225. doi

Insert

NAD-cpLuc2 sensor

Use

Tags

Hisx10 and Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCyPet-Rac1(T17N)

Plasmid

#22784

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (T17N) (RAC1 Human, A. victoria)

Use

Tags

CyPet

Expression

Mammalian

Mutation

Rac1 T17N.

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pET-51b-HaloTag10

Plasmid

#175520

Purpose

Expression of HaloTag10 in bacterial cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag10

Use

Tags

His

Expression

Bacterial

Mutation

HaloTag10 = HaloTag7-Q165W

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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p3521 pET ubcH6

Plasmid

#8653

Depositor

Peter Howley

Article

Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

Insert

UbcH6 (UBE2E1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only