We narrowed to 162,676 results for: addgene

1. SP_A5

   Plasmid

   #228149

   Purpose

   Plasmid contains the secretion signal peptide sequence of the human GDF8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

   Depositor

   Joseph Brock

   Article

   Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

   Insert

   sp_O14793_GDF8_HUMAN:0-18
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. MIGR1-U6gRNA1-Filler-v1

   Plasmid

   #237399

   Purpose

   For inserting a single guide RNA or later cloning two U6-gRNA cassettes with MIGR1-U6gRNA1-Filler-v2.

   Depositor

   Zuoming Sun

   Article

   Zhong et al STAR Protoc. 2025 Jun 20;6(2):103831. do

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, PGK

   Available since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. MIGR1-U6gRNA1-Filler-v2

   Plasmid

   #237400

   Purpose

   For inserting a single guide RNA or later cloning two U6-gRNA cassettes with MIGR1-U6gRNA1-Filler-v1.

   Depositor

   Zuoming Sun

   Article

   Zhong et al STAR Protoc. 2025 Jun 20;6(2):103831. do

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, PGK

   Available since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-mScarlet-SH3PXD2A

   Plasmid

   #215534

   Purpose

   Expression vector with Tks5 tagged with N-terminal mScarlet

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   SH3PXD2A (SH3PXD2A Human)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pINDUCER20-PTPN12

   Plasmid

   #215517

   Purpose

   Dox-inducible lentiviral transfer vector with PTP-PEST

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   PTPN12 (PTPN12 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mCMV, Doxycycline-inducible

   Available since

   May 29, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pX458-MYADM

   Plasmid

   #235245

   Purpose

   Encodes gRNA for human MYADM

   Depositor

   Jan Carette

   Article

   Qiao et al Nat Commun. 2024 Apr 24;15(1):3469. doi:

   Insert

   MYADM (MYADM Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB2188-PrARG8-GFP::URA3

   Plasmid

   #216512

   Purpose

   Integrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

   Depositor

   Juha Saarikangas

   Article

   Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

   Insert

   PrARG8-yeGFP
   

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ARG8

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB2197-PrGNP1-mK2-NatMX

   Plasmid

   #216513

   Purpose

   Integrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

   Depositor

   Juha Saarikangas

   Article

   Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

   Insert

   PrGnp1-mKate2
   

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GNP1

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX459-sgRNA047_Podxl

   Plasmid

   #232932

   Purpose

   Expression vector for a sgRNA against the mouse Podxl locus and SpCas9 with T2A-Puromycin resistance gene.

   Depositor

   Danny Reinberg

   Article

   Lucero et al bioRxiv, March 19, 2025

   Insert

   Cas9-T2A-PuroR (Podxl S. pyogenes)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX458-eBFP2-sgRNA_CTCF_ZF1

    Plasmid

    #233090

    Purpose

    Expression vector for a sgRNA against the mouse CTCF ZF1 region and SpCas9-T2A-eBFP2.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    spCas9-T2A-eBFP2 (Ctcf )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG415Gal_Gcn4 SATtoG KtoRHA

    Plasmid

    #232994

    Purpose

    Galactose iduced expression of Gcn4 SATtoG KtoRHAin yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 SATtoG KtoR
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG415Gal_Gcn4 SATtoG KtoR

    Plasmid

    #232961

    Purpose

    Galactose iduced expression of Gcn4 ATtoG KtoR in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 SATtoG KtoR
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG415Gal_Gcn4 ILVtoED solvvol

    Plasmid

    #232958

    Purpose

    Galactose iduced expression of Gcn4 LVtoED solvvol in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED solvvol
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG415Gal_Gcn4 SATtoG KtoRGFP

    Plasmid

    #233006

    Purpose

    Galactose iduced expression of Gcn4 SATtoG KtoRGFP in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 SATtoG KtoR
    

    Use

    Tags

    HA-GFP-HA and TEV cleavage site

    Expression

    Yeast

    Mutation

    S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG415Gal_Gcn4 solvvol W

    Plasmid

    #232977

    Purpose

    Galactose iduced expression of Gcn4 solvvol W+ in yeast

    Depositor

    Tanja Mittag, Aseem Ansari

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 solvvol W+
    

    Use

    Tags

    3xHA and TEV cleavage site

    Expression

    Yeast

    Mutation

    S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCY5h

    Plasmid

    #160295

    Purpose

    Yeast CRISPR plasmid targeting the hphMX cassette

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCY5n

    Plasmid

    #160296

    Purpose

    Yeast CRISPR plasmid targeting the natMX cassette

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCY5k

    Plasmid

    #160294

    Purpose

    Yeast CRISPR plasmid targeting the kanMX cassette

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHW1133

    Plasmid

    #229869

    Purpose

    Destination RMCE vector containing FRT::SA::51-bp Artificial exon::3xStop::SL2::SapI Insertion sites (to clone new driver with 3'UTR in slot 5 and SEC in slot 6)::reverse FRT3 to swap driver via RMCE

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. N-Terminal Halo XRCC4

    Plasmid

    #207541

    Purpose

    Homologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    HaloTag with internal PuroR cassette flanked by human XRCC4 locus sequences
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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