Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 15,864 results for: cas9

Clear keywords
Showing: 3881 - 3900 of 15864 results

  5. DNMT3L-dCas9

    Plasmid
    #126587
    Purpose
    DNMT3L fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNMT3L (DNMT3L Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-DNMT3L-dCas9-NLS
    Expression
    Mammalian
    Available since
    Dec. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    126587-plasmid-map-sequence-id-244969

    Enlarge

  7. pCBh-T7-SpCas9(KRTMQR)-P2A-mTagBFP2(RAS3591)

    Plasmid
    #223106
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with KRTMQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KRTMQR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(KRTMQR)=D1135K, S1136R, G1218T, E1219M, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223106-plasmid-map-sequence-id-447626

    Enlarge

  8. pCBh-T7-SpCas9(KRHWMR)-P2A-mTagBFP2(RAS3594)

    Plasmid
    #223107
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KRHWMR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(KRHWMR)=D1135K, S1136R, G1218H, E1219W, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223107-plasmid-map-sequence-id-447623

    Enlarge

  9. pCBh-T7-SpCas9(MRRWYR)-P2A-mTagBFP2(RAS3598)

    Plasmid
    #223108
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with MRRWYR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRRWYR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(MRRWYR)=D1135M, S1136R, G1218R, E1219W, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223108-plasmid-map-sequence-id-447631

    Enlarge

  10. pCBh-T7-SpCas9(MRRWMR)-P2A-mTagBFP2(RAS3579)

    Plasmid
    #223109
    Purpose
    pCBh Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRRWMR)-P2A-mTagBFP2
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223109-plasmid-map-sequence-id-447628

    Enlarge

  11. pCMV-T7-SpCas9(KRHWMR)-P2A-EGFP_(RAS3808)

    Plasmid
    #228251
    Purpose
    pCMV human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KRHWMR)-P2A-EGFP
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(ARGIMR)=D1135K, S1136R, G1218H, E1219W, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    228251-plasmid-map-sequence-id-451023

    Enlarge

  14. pEf1a-superDn29-dCas9-P2A-GFP

    Plasmid
    #247156
    Purpose
    Mammalian expression of a human codon optimized engineered superDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3
    Depositor
    Patrick Hsu
    Publication
    Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    superDn29-dCas9-P2A-GFP
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…
    Promoter
    Ef1a
    Available since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    247156-plasmid-map-sequence-id-491689

    Enlarge

  17. EF1a-TET1-dCas9

    Plasmid
    #235593
    Purpose
    Tet1CD fused to N-terminus of dCas9
    Depositor
    Ting Zhou
    Publication
    Wu et al Stem Cell Reports. 2025 Apr 25:102499. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)
    Use
    CRISPR
    Tags
    3xNLS, TET1 CD, and tagBFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    235593-plasmid-map-sequence-id-467826

    Enlarge

  18. SpCas9-YE1-BE4max

    Plasmid
    #198546
    Purpose
    Expresses human codon-optimized SpCas9-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-YE1-BE4max-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-YE1-BE4max
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198546-plasmid-map-sequence-id-390295

    Enlarge

Showing: 3881 - 3900 of 15864 results