We narrowed to 13,725 results for: cas9

1. vHB16

   Plasmid

   #193455

   Purpose

   Expression of GFP-Cas9-NLS and gRNA in A. castellanii

   Depositor

   Chantal Abergel

   Article

   Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

   Insert

   tRNA-gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX854

   Plasmid

   #62886

   Purpose

   C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa573-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS SV40, and NLS nucleoplasmin

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCJ1021

   Plasmid

   #228761

   Purpose

   Plasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Ift88 gRNAs (Ift88 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJH1

   Plasmid

   #154204

   Purpose

   encodes CRISPR-Cas9 system derived from plasmid pCas9

   Depositor

   Russell Poulter

   Article

   Ho et al J Med Microbiol. 2020 Mar;69(3):478-486.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti SpBsmBI sgRNA Puro

   Plasmid

   #62207

   Purpose

   An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)

   Depositor

   Rene Maehr

   Article

   Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PX853

   Plasmid

   #62885

   Purpose

   N-term SpCas9 piece of inducible split-5 (Cas9(N)-FRB-NES split-5).

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa 2-573)
   

   Use

   CRISPR

   Tags

   FRB and NES PTK2

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYZ301

   Plasmid

   #102690

   Purpose

   S. pombe gRNA entry vector with Cas9 - Sp lys9 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYZ294

   Plasmid

   #102688

   Purpose

   S. pombe gRNA entry vector with Cas9 - KanMX marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYZ300

   Plasmid

   #102689

   Purpose

   S. pombe gRNA entry vector with Cas9 - Sp his3 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYZ293

    Plasmid

    #102687

    Purpose

    S. pombe gRNA entry vector with Cas9 - Sp leu1 marker

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKI1.1

    Plasmid

    #85807

    Purpose

    CRISPR/Cas9 for Arabidopsis in pENTR/D-TOPO

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mutation

    Promoter

    AtRPS5A

    Available since

    May 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR1002

    Plasmid

    #71820

    Purpose

    Express Streptococcus pyogenes Cas9

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_10xHis-MBP-TEV-SPyCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2CT-Cdi

    Plasmid

    #83044

    Purpose

    Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

    Depositor

    Jennifer Doudna

    Article

    Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

    Insert

    CdiCas9
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

14. TU6m-gRNAscaffHygroR

    Plasmid

    #165485

    Purpose

    S. pyogenes Cas9 guide RNA expression in tilapia cells

    Depositor

    Dietmar Kueltz

    Article

    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Oreochromis mossambicus U6

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBC2101-reci

    Plasmid

    #117170

    Purpose

    U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 with NLS

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    reci_wtCas9 with SV40 NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCJ1022

    Plasmid

    #228762

    Purpose

    Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Pkd2 gRNAs (Pkd2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCP

    Plasmid

    #204743

    Purpose

    Cas9-2A-Puro expression vector

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTW072

    Plasmid

    #190247

    Purpose

    T-DNA for arabidopsis transformation

    Depositor

    Feng Zhang

    Article

    Weiss et al Plant Physiol. 2022 Jun 11. pii: 6605859

    Insert

    gRNA, Cas9, firefly luciferase, bar
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTW069

    Plasmid

    #190246

    Purpose

    T-DNA for arabidopsis transformation

    Depositor

    Feng Zhang

    Article

    Weiss et al Plant Physiol. 2022 Jun 11. pii: 6605859

    Insert

    gRNA, Cas9, firefly luciferase, bar
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSPneoSagRNAH

    Plasmid

    #123265

    Purpose

    Expresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

    Type

    Empty backbone

    Use

    CRISPR; Leishmania expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge