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EF1a_HNF4A_P2A_Hygro_Barcode

Plasmid

#120450

Purpose

Barcoded lentiviral vector to express HNF4A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HNF4A (HNF4A Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGTag-TagRFP-SV40

Plasmid

#117807

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Publication

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pB-CAGGS-dCas9

Plasmid

#110823

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, George Church

Publication

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCS6-YFP-SMASh(AI)

Plasmid

#111500

Purpose

Expresses Venus YFP with a C-terminal SMASh(AI) tag fusion in mammalian cells. [AI = asunaprevir inhibited].

Depositor

Michael Lin

Publication

Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

Insert

YFP-SMASh(AI)

Tags

HA epitope tag and SMASh tag

Expression

Mammalian

Mutation

The HCV NS3 protease carries V36M, T54A, and S122…

Promoter

CMV

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDNA2.0 6H-TEV-KRAS G12V

Plasmid

#111850

Purpose

Expresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

Depositor

Kenneth Westover

Publication

Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

Insert

KRAS G12V (KRAS Human)

Tags

6xHis-tag and TEV protease cleavage sequence

Expression

Bacterial

Mutation

Changed Glycine 12 to Valine

Promoter

T5

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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plenti-CAG-IKZF1-V1-FLAG-IRES-GFP

Plasmid

#107387

Purpose

Lentiviral expression of IKZF1-V1-FLAG

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF1-V1 (IKZF1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAG-ChroME-mRuby2-ST

Plasmid

#108902

Purpose

Cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

Depositor

Hillel Adesnik

Publication

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

ChroME-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-dCpf1-eBE

Plasmid

#107688

Purpose

Expresses dLbCpf1-eBE in mammalian cells

Depositor

Jia Chen

Publication

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-eBE

Expression

Mammalian

Mutation

dLbCpf1(D832A/E925A/D1148A)

Promoter

CMV

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiHer24D5-3_synNotch_Gal4VP64

Plasmid

#85422

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-3 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Publication

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her_4D5-3 synNotch

Use

Lentiviral

Promoter

PGK

Available since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMH-MYC

Plasmid

#101765

Purpose

Gateway-compatible destination vector for mammalian expression of N-terminal Myc-tag fusion protein

Depositor

Michael Huen

Publication

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Tags

Myc

Expression

Mammalian

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDONR223_EGFR_WT

Plasmid

#81926

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EGFR (EGFR Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_DNMT3A_WT

Plasmid

#81765

Purpose

Gateway Donor vector containing DNMT3A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

DNMT3A (DNMT3A Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G12C

Plasmid

#81667

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

G12C

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223_MDM2_WT

Plasmid

#82897

Purpose

Gateway Donor vector containing MDM2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MDM2 (MDM2 Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGLO-GFP-1UAG

Plasmid

#82500

Purpose

Expresses GFP with 1 UAG codon at the amino acid position 3

Depositor

Chang Liu

Publication

Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

Insert

Green Fluorescent Protein with 1 UAG codon

Expression

Bacterial

Mutation

Added an UAG codon at the amino acid position 3

Promoter

AraBAD

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHDE-35S-Cas9-mCherry

Plasmid

#78931

Purpose

For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants

Depositor

Yunde Zhao

Publication

Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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His6TEV-UbG76C

Plasmid

#75299

Purpose

Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

Depositor

Cynthia Wolberger

Publication

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

human Ubiquitin mutant G76 to C

Tags

His

Expression

Bacterial

Mutation

changed Glycine 76 to Cystine

Promoter

T7

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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MEK1 (S218D,S222D)-pcw107

Plasmid

#64604

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Mutation

S218D, S222D

Promoter

PGK

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GFP-PHIP

Plasmid

#65389

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Publication

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

PHIP (PHIP Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pCMU-TPr

Plasmid

#61189

Purpose

Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtTIP1-1

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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