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pHis-LMW_FGF2

Plasmid

#157657

Purpose

Expresses FGF2 in E.coli cell

Depositor

Byung Il Lee

Publication

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Fibroblast growth factor 2 (FGF2 Human)

Tags

His tag

Expression

Bacterial

Mutation

changed Cystein 211,229 to serine, deleted amino …

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-ABEmaxCP1041-BlastR

Plasmid

#152990

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

ABE7.10-nSpCas9 CP1041

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p2T-CMV-BE4max-BlastR

Plasmid

#152991

Purpose

C-to-T base editor

Depositor

David Liu

Publication

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

BE4-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TB:7xSMAD_RE:MiniP

Plasmid

#133879

Purpose

7 copies the SMAD DNA binding motif

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

7 copies the SMAD DNA binding motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z22

Plasmid

#120065

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P15

Plasmid

#120026

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

miRFP670

Use

Pcr template

Tags

miRFP670

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP

Plasmid

#114729

Purpose

3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc gene

Depositor

Martine Roussel

Publication

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

sgRNA M5, sgRNA M7, sgRNA M9

Use

CRISPR

Expression

Mammalian

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Kohinoor-Clathrin

Plasmid

#67773

Purpose

Clathrin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Publication

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

Clathrin

Expression

Mammalian

Promoter

CMV

Available since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Kohinoor-Vimentin/pcDNA3

Plasmid

#67772

Purpose

Vimentin-targeted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Publication

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor-Vimentin

Tags

Vimentin

Expression

Mammalian

Promoter

CMV

Available since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pTRE2-Hyg-omMx1

Plasmid

#52420

Purpose

Inducible expression (Tet-Off system) of the rainbow trout antiviral Mx1 protein

Depositor

Bertrand Collet

Publication

Lester et al J Virol Methods. 2012 Jun;182(1-2):1-8.

Insert

Oncorhynchus mykiss interferon-induced GTP-binding protein Mx1 (mx1)

Expression

Mammalian

Promoter

TRE2

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV10-Flag-Fbxl3

Plasmid

#48751

Purpose

Expression of FLAG-tagged Fbxl3

Depositor

Joseph Takahashi

Publication

Siepka et al Cell. 2007 Jun 1;129(5):1011-23. Epub 20

Insert

Fbxl3 (Fbxl3 Mouse)

Tags

3XFLAG

Expression

Mammalian

Available since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
PB6K-mcherry-iATPsnFR1.1

Plasmid

#227239

Purpose

Inducible ATP sensor for Pseudomonas putida

Depositor

Fuzhong Zhang

Publication

Mu et al Nat Commun. 2024 Jun 21;15(1):5299. doi:

Insert

iATPsnFR1.1

Expression

Bacterial

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCFJ108 - pENTR221 - [cbr-unc-119(+)]

Plasmid

#200367

Purpose

cbr-unc-119 rescue gene for use as co-injection marker.

Depositor

Christian Froekjaer-Jensen

Publication

AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

Insert

[cbr-unc-119(+)]

Expression

Worm

Available since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBBR1_DVK_AE

Plasmid

#188946

Purpose

Level 1 destination vector containing pBBR1 origin of replication and CIDAR AE scars

Depositor

Eric Young

Publication

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVS_AF

Plasmid

#188950

Purpose

Level 2 destination vector containing pBBR1 origin of replication and CIDAR AF scars

Depositor

Eric Young

Publication

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR1_DVC_AG

Plasmid

#188954

Purpose

Level 2 destination vector containing pBBR1 origin of replication and CIDAR AG scars

Depositor

Eric Young

Publication

Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pIT5_KH

Plasmid

#173772

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Publication

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pIT5_SL

Plasmid

#173769

Purpose

Bacterial genomic integration vector

Depositor

Keith Shearwin

Publication

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-GFP

Plasmid

#166836

Purpose

Expresses Vac8-GFP in yeasts.

Depositor

Daniel Klionsky

Publication

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

VAC8 promoter

Available since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pNM3813

Plasmid

#154121

Purpose

mec-4 promoter driving lexA-L-QFAD in an RCME vector

Depositor

Michael Nonet

Publication

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::lexA-L-QFAD::act-4 '3

Use

Rmce

Expression

Worm

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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