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MSCV-6NBC-GFP-PGK-Puro-Barcode9

Plasmid

#83686

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode9

Use

Retroviral

Mutation

please view depositor comments below

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode7

Plasmid

#83684

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode7

Use

Retroviral

Mutation

L119P in GFP

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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UAS-Xbp1spliced

Plasmid

#80628

Purpose

expression of Xbp1 RB isoform in Drosophila

Depositor

Pedro Domingos, Herman Steller

Article

Ryoo et al EMBO J. 2007 Jan 10;26(1):242-52. Epub 2

Insert

Xbp1 (Xbp1 Fly)

Expression

Insect

Mutation

RB isoform

Promoter

hsp70

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only
pNH (GB0064)

Plasmid

#68165

Purpose

Provides the S. Lycopersicum fruit promoter NH as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlNH promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPUbc (GB0145)

Plasmid

#68170

Purpose

Provides the S. Lycopersicum Ubiquitin carrier protein promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlUbc promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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p2x35S (GB0222)

Plasmid

#68173

Purpose

Provides the CaMV dual 35S Promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

2x35S promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only
p6xUAS (GB0179)

Plasmid

#68182

Purpose

Provides 6xUAS (upstream activating sequence) as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

6xUAS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pJK286

Plasmid

#71567

Purpose

Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (UctB)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

formyl-CoA:oxalate CoA-transferase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TCEB3B

Plasmid

#67117

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCEB3B (ELOA2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 ZFYVE9

Plasmid

#67134

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

ZFYVE9 (ZFYVE9 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

F1359I

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pDGB3_omega1R

Plasmid

#68240

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pT2A11 (GB0143)

Plasmid

#68189

Purpose

Provides the S. Lycopersicum 2A11 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Sl2A11 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pTE8 (GB0144)

Plasmid

#68190

Purpose

Provides the S. Lycopersicum E8 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlE8 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLexAOp (GB0118)

Plasmid

#68181

Purpose

Provides the 4xLexA Operator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

4xOpLexA

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB3_omega2R

Plasmid

#68241

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET11+PCNA3-CYP119

Plasmid

#66136

Purpose

Expresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Tags

Sulfolobus solfataricus PCNA3

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET11+PCNA3-CYP119(T214V)

Plasmid

#66137

Purpose

Expresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Tags

Sulfolobus solfataricus PCNA3

Expression

Bacterial

Mutation

T214V

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET11+PCNA3-CYP119(D77R/T214V)

Plasmid

#66138

Purpose

Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Tags

Sulfolobus solfataricus PCNA3

Expression

Bacterial

Mutation

D77R, T214V

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pBGSA FPR1 R123A

Plasmid

#66721

Purpose

Constitutive expression of FPR1 R123A in mammalian cells.

Depositor

Heini Miettinen

Article

Miettinen et al J Biol Chem. 1999 Sep 24;274(39):27934-4

Insert

FPR1 (haplotype 3) (FPR1 Human)

Expression

Mammalian

Mutation

R123A

Promoter

SR-alpha

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pBGSA FPR1 D71A

Plasmid

#66720

Purpose

Constitutive expression of FPR1 D71A in mammalian cells.

Depositor

Heini Miettinen

Article

Miettinen et al J Biol Chem. 1999 Sep 24;274(39):27934-4

Insert

FPR1 (haplotype 3) (FPR1 Human)

Expression

Mammalian

Mutation

D71A

Promoter

SR-alpha

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pBGSA FPR1 H4 (haplotype 4)

Plasmid

#66718

Purpose

Constitutive expression of FPR1 in mammalian cells

Depositor

Heini Miettinen

Article

Gripentrog et al Immunogenetics. 2008 Feb;60(2):83-93. do

Insert

FPR1 (haplotype 4) (FPR1 Human)

Expression

Mammalian

Promoter

SR-alpha

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pBGSA FPR1 N297A

Plasmid

#66723

Purpose

Constitutive expression of FPR1 N297A in mammalian cells.

Depositor

Heini Miettinen

Article

Gripentrog et al Cell Signal. 2008 Feb;20(2):424-31. Epub

Insert

FPR1 (haplotype 3) (FPR1 Human)

Expression

Mammalian

Mutation

N297A

Promoter

SR-alpha

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pBGSA FPR1 Q258A

Plasmid

#66724

Purpose

Constitutive expression of FPR1 Q258A in mammalian cells.

Depositor

Heini Miettinen

Article

Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

Insert

FPR1 (haplotype 3) (FPR1 Human)

Expression

Mammalian

Mutation

Q258A

Promoter

SR-alpha

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only
pSG5-(nic)p85

Plasmid

#55720

Purpose

eukaryotic expression of both SH2 and the inter-SH2 domain of murine p85α.

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p85α

Expression

Mammalian

Mutation

SH2 and the inter-SH2 domain

Promoter

T7

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
Myc-CICf_AEA

Plasmid

#48188

Purpose

Expresses AEA mutated Cic

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Capicua (Cic Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-103 Y96F

Plasmid

#47094

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y96F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y96F

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-103 Y32F

Plasmid

#47091

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y32F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y32F

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 Y96F

Plasmid

#47085

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-09 Y96F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y96F

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 Y91F

Plasmid

#47084

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-09 Y91F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y91F

Promoter

T7

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 Y32F Y96F

Plasmid

#47083

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-09 Y32F Y96F (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Y32F Y96F

Promoter

T7

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only