We narrowed to 11,805 results for: NSI;
-
Plasmid#217448PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1DepositorAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only
-
HIV-1-GFP ΔEnv CA Q63/67A
Plasmid#217439PurposeHIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q63A,Q67A mutations in CA).DepositorInsertgag/pol
UseLentiviralMutationCA Q63A,Q67AAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
HIV-1-GFP ΔEnv CA Q67H/N74D
Plasmid#217441PurposeHIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef. Contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q67H,N74D mutations in CA).DepositorInsertgag/pol
UseLentiviralMutationCA Q67H,N74DAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
LCV2 PQBP1 KO1
Plasmid#217447PurposeLentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1DepositorAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-WIPI1promotor
Plasmid#206840PurposeThe plasmid contains the human WIPI1 promotor sequence (-1226 bp upstream to +329 bp downstream of the transcription start site) cloned into pGL4.23[luc2/minP] (NheI/HindIII).DepositorInsertWIPI1 promotor (partial)
UseLuciferaseAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pINQ-ON10
Plasmid#198689PurposePeriplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)DepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody ON10
Tags6xHis tag, HA tag, OmpA signal peptide, and Ribos…ExpressionBacterialPromoterT7 promoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINQ-NbH4
Plasmid#198690PurposePeriplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)DepositorInsertanti-SARS-CoV-2 Nucleocapsid nanobody H4
Tags6xHis tag, Avitag, OmpA signal peptide, and Ribos…ExpressionBacterialPromoterT7 promoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP6 145-201
Plasmid#104597PurposeExpresses C-terminal 145-201 residues of CHMP6 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-11DepositorAvailable SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP7 366-453
Plasmid#104598PurposeExpresses C-terminal 366-453 residues of CHMP7 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-12DepositorAvailable SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CHMP4A 153-222
Plasmid#108256PurposeExpresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6DepositorInsertCHMP4A residues 153-222 (CHMP4A Human)
TagsHis-SUMOExpressionBacterialMutationContains nonnative Gly-Cys for fluorescent labeli…PromoterT7Available SinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICH86966::AtU6p::sgRNA:NbCysP6
Plasmid#223217PurposeExpresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoterDepositorInsertNbCysP6 sgRNA
UseCRISPR and Synthetic BiologyTagsNoneExpressionPlantPromoterArabidopsis U6 promoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCrhaB2_ApyD_ RBS_ApyAOHIS
Plasmid#226247PurposeProduction of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApySDepositorInsertsHis6_ApyA
ApyD
ApyO
ApyH
ApyI
ApyS
TagsHexa-HistidineExpressionBacterialAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAT-4_Crp
Plasmid#217168PurposeMammalian expression vector for expression of hAT-4_Crp transposase (CMV promoter)DepositorInserthAT-4_Crp transposase
ExpressionMammalianAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAT-1_AMi
Plasmid#217170PurposeMammalian expression vector for expression of hAT-1_Ami transposase (CMV promoter)DepositorInserthAT-1_AMi transposase
ExpressionMammalianAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Mariner-2_AMi
Plasmid#217171PurposeMammalian expression vector for expression of Mariner-2_Ami transposase (CMV promoter)DepositorInsertMariner-2_AMi transposase
ExpressionMammalianAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAT-13_AMi
Plasmid#217172PurposeMammalian expression vector for expression of hAT-13_Ami transposase (CMV promoter)DepositorInserthAT-13_AMi transposase
ExpressionMammalianAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
LT3GEPIR-CCND1shRNA3
Plasmid#220579PurposeTo inducibly knockdown CCND1 expressionDepositorAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-C1-Nir1-631-894
Plasmid#220089PurposeTruncated Nir1-LNS2 domain lacking N-terminal amphipathic helix; loses ability to bind PADepositorInsertPITPNM3 (PITPNM3 Human)
TagsNeonGreen-GGSGGExpressionMammalianMutationamino acids 631-894PromoterCMVAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC1_oROS-HT_LF(C199S)
Plasmid#216412PurposeExpresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.DepositorInsertoROS-HT_LF(C199S)
ExpressionMammalianPromoterCMVAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC1-lifeact-oROS-HT
Plasmid#216420PurposeTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.DepositorInsertlifeact-oROS-HT
ExpressionMammalianPromoterCMVAvailable SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-N1-Nir1-613-630
Plasmid#220088PurposeN-terminal amphipathic helix of Nir1-LNS2 domain; loses ability to bind PADepositorInsertPITPNM3 (PITPNM3 Human)
TagsGGSGG-NeonGreenExpressionMammalianMutationamino acids 613-630PromoterCMVAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-C Sars-CoV-2 E P71L
Plasmid#194804PurposeSars-CoV-2 E_P71L in MAC-C destination vectorDepositorAvailable SinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-C Sars-CoV-2 N_D3L
Plasmid#194806PurposeSars-CoV-2 N_D3L in MAC-C destination vectorDepositorAvailable SinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-C Sars-CoV-2 N_D63G
Plasmid#194807PurposeSars-CoV-2 N_D63G in MAC-C destination vectorDepositorAvailable SinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-C Sars-CoV-2 N_R203M
Plasmid#194810PurposeSars-CoV-2 N_R203M in MAC-C destination vectorDepositorAvailable SinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC-C Sars-CoV-2 N_S235F
Plasmid#194811PurposeSars-CoV-2 N_S235F in MAC-C destination vectorDepositorAvailable SinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
HA-C Sars-CoV-2 N_T205I
Plasmid#194812PurposeSars-CoV-2 N_T205I in MAC-C destination vectorDepositorAvailable SinceMay 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pIRHDGAL43C
Plasmid#218279PurposeIntroduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
ExpressionYeastMutationGAL3 (S509P), DHFR (P66L)Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRHDGAL3C
Plasmid#218276PurposeIntroduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
ExpressionYeastMutationGAL3 (S509P), DHFR (P66L)Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AtSLAC1 WT
Plasmid#212946PurposeVector used for expression of AtSLAC1 WT in mammalian cellDepositorAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AtSLAC1 6D
Plasmid#212947PurposeVector used for expression of AtSLAC1 6D in mammalian cellDepositorInsertSlow anion channel-associated 1 (OZS1 Mustard Weed)
ExpressionMammalianMutationT62D/S65D/S107D/S124D/S146D/S152DPromoterCMVAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-hALDOA D34S
Plasmid#210813PurposeTo reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)DepositorInsertALDOA (ALDOA Human)
UseAdenoviralTagsHAMutationresidues 34 of ALDOA mutated to serinePromoterCMVAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hALDOA K230A
Plasmid#210811PurposeTo block the formation of the initial Schiff base with FBPDepositorInsertALDOA (ALDOA Human)
TagsHAExpressionMammalianMutationresidues 230 of ALDOA mutated to alaninePromoterCMVAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBoBi-hALDOA D34S
Plasmid#210807PurposeTo reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)DepositorInsertALDOA (ALDOA Human)
UseLentiviralTagsHAMutationresidues 34 of ALDOA mutated to serinePromoterCMVAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_6F→A
Plasmid#194269PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 6F→ADepositorInsertTDP-43 (TARDBP Synthetic, Human)
Tags6XHistagExpressionBacterialMutationF276A, F283A, F289A, F367A, F397A, F401APromoterT7Available SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→A_CR
Plasmid#194263PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5M→A_CRDepositorInsertTDP-43 (TARDBP Synthetic, Human)
Tags6XHistagExpressionBacterialMutationM322A, M323A, M336A, M337A, M339A mutations in TD…PromoterT7Available SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→A_IDR
Plasmid#194264PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to AlanineDepositorInsertTDP-43 (TARDBP Synthetic, Human)
Tags6XHistagExpressionBacterialMutationM307A, M311A, M359A, M405A, M414A mutations in TD…PromoterT7Available SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→L_IDR
Plasmid#194265PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to leucineDepositorInsertTDP-43 (TARDBP Human)
Tags6XHistagExpressionBacterialMutationM307L, M311L, M359L, M405L, M414L mutations in TD…PromoterT7Available SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5M→Y_IDR
Plasmid#194266PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to tyrosineDepositorInsertTDP-43 (TARDBP Synthetic, Human)
Tags6XHistagExpressionBacterialMutationM307Y, M311Y, M359Y, M405Y, M414Y mutations in TD…PromoterT7Available SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-43 _CTD_5A→M_IDR
Plasmid#194267PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Alanines to methionineDepositorInsertTDP-43 (TARDBP Synthetic, Human)
Tags6XHistagExpressionBacterialMutationA297M, A367M, A381M, A388M, A391M mutations in TD…PromoterT7Available SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only