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pGEX SYT9 C2A

Plasmid

#195707

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 124-211 of mouse SYT9

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2B

Plasmid

#195708

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 253-344 of mouse SYT9

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2AB

Plasmid

#195709

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 104-386 of mouse SYT9

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT1 C2A

Plasmid

#195710

Purpose

Bacterial expression plasmid encoding GST-tagged SYT1 C2A domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 96-264 of mouse SYT1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT1 C2B

Plasmid

#195711

Purpose

Bacterial expression plasmid encoding GST-tagged SYT1 C2B domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 248-421 of mouse SYT1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT1 C2AB

Plasmid

#195712

Purpose

Bacterial expression plasmid encoding GST-tagged SYT1 C2AB domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt1 (Syt1 Rat)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 96-421 of mouse SYT1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET (SUMO)-Syt9

Plasmid

#195713

Purpose

Bacterial expression plasmid encoding SUMO-tagged full-length Syt9

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Rat)

Tags

GST-SUMO

Expression

Bacterial

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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UAS-GFP::NMN-Deamidase

Plasmid

#187868

Purpose

Gal4/UAS expression of GFP::NMN-Deamidase

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

NMN-Deamidase

Tags

GFP

Expression

Insect

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-dsarm(E)::flag

Plasmid

#187871

Purpose

Gal4/UAS expression of dSarm(isoE)::FLAG

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

dSarm isoform E (Sarm Fly)

Tags

FLAG

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-dsarm(E,K450R)

Plasmid

#187881

Purpose

Gal4/UAS expression of dSarm(isoE,K450R)

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

dSarm isoform E (Sarm )

Mutation

K450R

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pX330A-Yap1-E1-#2

Plasmid

#171519

Purpose

deletion of a genomic locus in Yap1 gene

Depositor

Hans Schöler

Article

Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

Insert

Yap1 (Yap1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-LexA-CIB1-biLINuS

Plasmid

#192607

Purpose

Lentiviral vector for constitutive expression of LexA-CIB1-biLINuS

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

LexA-CIB1-biLINuS

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ND6-L1397-N_Q1310A_right

Plasmid

#187849

Purpose

expresses the right-side half ND6-Q1310A in mammalian cells

Depositor

Chengqi Yi

Article

Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

Insert

COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–ATP5B 3'UTR

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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ND6-L1397-N_Q1310A_left

Plasmid

#187848

Purpose

expresses the left-side half ND6-Q1310A in mammalian cells

Depositor

Chengqi Yi

Article

Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

Insert

SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N(Q1310A)–UGI–SOD2 3'UTR

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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bpNLS-DddIA

Plasmid

#187847

Purpose

expresses the bpNLS-linked DddIA in mammalian cells

Depositor

Chengqi Yi

Article

Lei et al Nature. 2022 May 12. pii: 10.1038/s41586

Insert

bpNLS-DddIA-bpNLS-p2A-EGFP

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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CAG.MCAK-mCherry-eDHFR

Plasmid

#176204

Purpose

MCAK (179-583) recruitment responsible for targeted microtubule depolymerization using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

MCAK-mCherry-eDHFR

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-His-TEV-OTC_d1_Ub

Plasmid

#170729

Purpose

Scaﬀold design for studying ubiquitin interacting proteins by cryo-EM.

Depositor

Shang-Te Danny Hsu

Article

Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

Insert

E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin

Tags

6xHis-TEV

Expression

Bacterial

Mutation

The C-terminus of OTC was truncated by removing o…

Promoter

T7

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 FIIND (F1079M-L1373)

Plasmid

#166831

Purpose

Gateway pDONR vector containing NLRP1 FIIND (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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dTAG NLRP1 P1214R

Plasmid

#166826

Purpose

dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Expression

Mammalian

Mutation

P1214R

Promoter

hPGK

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj1b-IRES-EGFP

Plasmid

#164946

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj1b and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj1b (kcnj1b Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Promoter

actinb (actb2) promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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