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We narrowed to 6,371 results for: siae

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Showing: 5911 - 5940 of 6371 results

  6. pRS424-TUB2 - human TUBB4a Cterminal tail C127S, E435C

    Plasmid
    #60405
    Purpose
    Expression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) C127S, E435C for crosslinking polyglutamate peptide
    Depositor
    Ron Vale
    Article
    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
    Insert
    TUB2
    Expression
    Yeast
    Mutation
    TUB2 amino acids 429 - end, replaced with human T…
    Promoter
    GAL
    Available Since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pAV10.F3.loxP

    Plasmid
    #63200
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63200-plasmid-map-sequence-id-104877

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  8. pAV10.HO5.loxP

    Plasmid
    #63206
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Availability
    Academic Institutions and Nonprofits only
    63206-plasmid-map-sequence-id-104906

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  11. pHES839

    Plasmid
    #87941
    Purpose
    Constitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.
    Depositor
    Hana El-Samad
    Article
    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102
    Insert
    GAL4 DBD - hER LBD - MSN2 AD (ESR1 Human, Budding Yeast, Synthetic)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    ADH1 (crippled)
    Available Since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87941-plasmid-map-sequence-id-180360

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  15. pcDNA3-Lyso-AktAR2

    Plasmid
    #64933
    Purpose
    Lysosome-targeted FRET biosensor for monitoring Akt activity; targeted to cytosolic face of lysosomal membrane.
    Depositor
    Jin Zhang
    Article
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Insert
    LAMP1-AktAR2 (LAMP1 Human, Budding Yeast, Synthetic)
    Tags
    Cerulean3, Full-length LAMP1, and cpVenus[E172]
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64933-plasmid-map-sequence-id-109712

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  16. Dronpa-FHA1

    Plasmid
    #87711
    Purpose
    Encodes N-terminal (PAABD) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR).
    Depositor
    Jin Zhang
    Article
    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi
    Insert
    Dronpa-FHA1
    Tags
    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    87711-plasmid-map-sequence-id-176141

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  18. pSEVA228-pro4IUPi

    Plasmid
    #122018
    Purpose
    Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.
    Depositor
    Gregory Stephanopoulos
    Article
    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p
    Inserts
    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    pro4
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    122018-plasmid-map-sequence-id-238966

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  20. Linked Free ClLIS1 (M)

    Plasmid
    #182487
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Insert
    FPPS(M)-LS
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182487-plasmid-map-sequence-id-360431

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  22. pcDNA3-Venus-cpVenus-FLARE-AKAR

    Plasmid
    #123329
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-AKAR
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    123329-plasmid-map-sequence-id-313436

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  24. pHES795

    Plasmid
    #87943
    Purpose
    Constitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.
    Depositor
    Hana El-Samad
    Article
    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102
    Insert
    Zif268 DBD - hER LBD - MSN2 AD (ESR1 Human, Budding Yeast, Synthetic)
    Expression
    Yeast
    Promoter
    ADH1 (crippled)
    Available Since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    87943-plasmid-map-sequence-id-189316

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  26. pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

    Plasmid
    #52890
    Purpose
    "CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.
    Depositor
    Iswar Hariharan
    Article
    Bosch et al Development. 2015 Feb 1;142(3):597-606.
    Inserts
    LexGAD
    Gal4 (GAL4 Budding Yeast)
    Expression
    Insect
    Available Since
    Jan. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    52890-plasmid-map-sequence-id-211914

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  30. pTET-IUPi

    Plasmid
    #122019
    Purpose
    Expresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.
    Depositor
    Gregory Stephanopoulos
    Article
    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p
    Inserts
    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    pTET
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    122019-plasmid-map-sequence-id-238972

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Showing: 5911 - 5940 of 6371 results