We narrowed to 4,679 results for: cre encoding plasmid

1. pKLV2-U6gRNA5(Pou5f1-g1)-PGKpuroBFP-W

   Plasmid

   #105035

   Purpose

   Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Pou5f1 (Pou5f1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKLV2-U6gRNA5(Kcmf1-g1)-PGKpuroBFP-W

   Plasmid

   #105017

   Purpose

   Lentiviral gRNA plasmid targeting mouse Kcmf1 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Kcmf1 (Kcmf1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 564aa ELL2 bluescript

   Plasmid

   #127267

   Purpose

   For in vitro translation of human ELL2 lacking COOH region

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   Addgene Plasmid 127256--SphI site filled in, then…

   Promoter

   T7

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCIneoRL-Notch1 3'UTR-mir200c-mut

   Plasmid

   #84596

   Purpose

   Renilla Luciferase reporter assay for NOTCH1 3'UTR with mutations on miR-200c binding site

   Depositor

   Yanan Yang

   Article

   Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

   Insert

   NOTCH1 3'UTR (NOTCH1 Human)
   

   Use

   Luciferase

   Tags

   Renilla Luciferase

   Expression

   Mutation

   Mutation on miR-200c binding site on NOTCH1 3…

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCIneoRL-LFNG 3'UTR-mir200c-mut

   Plasmid

   #84594

   Purpose

   Renilla Luciferase reporter assay for LFNG 3'UTR with mutations on miR-200c binding site

   Depositor

   Yanan Yang

   Article

   Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

   Insert

   LFNG 3'UTR (LFNG Human)
   

   Use

   Luciferase

   Tags

   Renilla Luciferase

   Expression

   Mutation

   Mutation on miR-200c binding site on LFNG 3'…

   Promoter

   CMV

   Available since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pU6-sgRosa26-1_CBh-Cas9-T2A-BFP

   Plasmid

   #64216

   Purpose

   Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA targeting ROSA26-1
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-PBZ-mRuby2

   Plasmid

   #110650

   Purpose

   expresses a PAR-binding (PBZ) domain fused to mRuby2

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

   Insert

   PBZ domain from APLF, amino acids 371-451 (APLF Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAcBac3-EcLeu TAG-EGFP**

   Plasmid

   #228785

   Purpose

   EcLeu aaRS/tRNA system for TAG suppression with EGFP containing two stop codons for dual suppression

   Depositor

   Abhishek Chatterjee

   Article

   Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

   Inserts

   EcLeuRS
   EcLtR
   EGFP**
   

   Use

   Baculoviral

   Tags

   Expression

   Mammalian

   Mutation

   TAG suppressor

   Promoter

   Available since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBiFC-PBZ-VN

   Plasmid

   #110646

   Purpose

   expresses a PAR-binding (PBZ) domain fused to venus N-terminus

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

   Insert

   PBZ domain from APLF, amino acids 371-451 (APLF Human)
   

   Use

   Tags

   DDK and VN

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EF1a_MYC deltaMBII_P2A_Hygro_Barcode

    Plasmid

    #120505

    Purpose

    Barcoded lentiviral vector to express c-MYC deltaMBII in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    c-MYC deltaMBII (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deletion of MYC Box II domain

    Promoter

    EF1a

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EF1a_KLF4_P2A_Hygro_Barcode

    Plasmid

    #120455

    Purpose

    Barcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKLV2-U6gRNA5(Stat3)-PGKpuroBFP-W

    Plasmid

    #105027

    Purpose

    Lentiviral gRNA plasmid targeting mouse Stat3 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pspCas9-3exo-Tetra-com-CLCN5-sp-g1

    Plasmid

    #176236

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

    Plasmid

    #176237

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pspCas9-Exo-tetra-com-hCLCN5-sp-g1

    Plasmid

    #176238

    Purpose

    Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Insert

    spCas9 and polymerase exo domain fusion (CLCN5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKLV2-U6gRNA5(Apc-g1)-PGKpuroBFP-W

    Plasmid

    #105022

    Purpose

    Lentiviral gRNA plasmid targeting mouse Apc , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Apc (Apc Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMT1219

    Plasmid

    #228301

    Purpose

    2,4-Diacetylphloroglucinol (DAPG)-regulatable expression of SARS-CoV-2 RBD omicron variant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    MFalpha(2xAdv)-SARS CoV-2 receptor binding domain (RBD)-His (S Severe acute respiratory syndrome-related coronavirus 2, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    GGG linker followed by His6 and MF_ secretion sig…

    Expression

    Yeast

    Mutation

    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …

    Promoter

    2,4-Diacetylphloroglucinol-regulatable synthetic …

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV.CBA.YFP.miR-E_shSrpk3-1

    Plasmid

    #180400

    Purpose

    Producing AAV that encodes mouse Srpk3 shRNA-1 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shSrpk3-1 (Srpk3 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV.CBA.YFP.miR-E_shSrpk3-2

    Plasmid

    #180401

    Purpose

    Producing AAV that encodes mouse Srpk3 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shSrpk3-2 (Srpk3 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV.CBA.YFP.miR-E_shSrpk3-3

    Plasmid

    #180402

    Purpose

    Producing AAV that encodes mouse Srpk3 shRNA-3 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shSrpk3-3 (Srpk3 Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge