Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 9,400 results for: Coli

Showing: 5941 - 5970 of 9400 results

  2. pRL1058

    Plasmid
    #70686
    Purpose
    Tn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.
    Depositor
    Peter Wolk
    Article
    Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88
    Insert
    Transposon Tn5-1058
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70686-plasmid-map-sequence-id-126440

    Enlarge

  6. pUC19-P70a-Top-T

    Plasmid
    #87312
    Purpose
    Expresses the "Top" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Split-Broccoli system "Top" transcript
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P70a
    Available Since
    Jan. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    87312-plasmid-map-sequence-id-201363

    Enlarge

  9. pFLIP30

    Plasmid
    #65499
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-AFPt9-attR1 and attR2-mCer-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available Since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65499-plasmid-map-sequence-id-111219

    Enlarge

  10. pMD19T-slr0230-PL31-sgRNANT1-KmR

    Plasmid
    #73221
    Purpose
    Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli
    Depositor
    Paul Hudson
    Article
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    PL31-sgRNA NT1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PL31
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73221-plasmid-map-sequence-id-135054

    Enlarge

  11. pTH09

    Plasmid
    #70064
    Purpose
    artificial transcription template "terminator insertion"
    Depositor
    Jeff Gelles
    Article
    Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi
    Insert
    lambda PR' - lambda TR2 - repeat cassette - E. coli rpoB - lambda TR'
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Jan. 7, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. pFLIP32

    Plasmid
    #65500
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available Since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65500-plasmid-map-sequence-id-111220

    Enlarge

  13. pFLIP39

    Plasmid
    #65507
    Purpose
    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Article
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Use
    Tags
    6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMyc
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available Since
    June 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    65507-plasmid-map-sequence-id-111416

    Enlarge

  15. pCMK83

    Plasmid
    #226793
    Purpose
    Integrates slr0214 (HIP1 methyltransferase) into gidB-atpI locus of E. coli DH10B
    Depositor
    David Nielsen
    Article
    Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:
    Insert
    slr0214 (slr0214 Synechocystis sp. PCC 6803)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tetracycline resistance cassette promoter (consti…
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    226793-plasmid-map-sequence-id-455428

    Enlarge

  20. pGEX-PHOSPHO2-TEV-Twin-Strep

    Plasmid
    #222017
    Purpose
    Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in E. coli
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO2 (PHOSPHO2 Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Bacterial
    Mutation
    deleted stop codon (TAA)
    Promoter
    lac promoter
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    222017-plasmid-map-sequence-id-457152

    Enlarge

Showing: 5941 - 5970 of 9400 results