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Showing: 3961 - 3980 of 5011 results

  1. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

    Plasmid
    #129032
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA4 (huTSDR)
    Use
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129032-plasmid-map-sequence-id-256156

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  2. pgRNA-humanized

    Plasmid
    #44248
    Purpose
    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    guide RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    mouse U6
    Available since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44248-plasmid-map-sequence-id-190219

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  4. pHR_SFFV*/Puro2a-GFP

    Plasmid
    #198222
    Purpose
    Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP
    Depositor
    Eric Davis
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Codon-optimized puromycin resistance gene, follow…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198222-plasmid-map-sequence-id-389408

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  8. pHW1387

    Plasmid
    #229880
    Purpose
    Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    Depositor
    Han Wang
    Article
    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.
    Insert
    Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    Use
    Tags
    GFP-C1
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    229880-plasmid-map-sequence-id-460861

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  9. pHW1392

    Plasmid
    #229878
    Purpose
    Negative selection marker for extrachromosomal arrays during RMCE crosses- Psnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    Depositor
    Han Wang
    Article
    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.
    Insert
    Psnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    Use
    Tags
    GFP-C1
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    229878-plasmid-map-sequence-id-460858

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  11. pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)

    Plasmid
    #114078
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
    Use
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114078-plasmid-map-sequence-id-220564

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  13. RANLS-LEHD-BNES

    Plasmid
    #60973
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A and ddRFP B
    Use
    Tags
    Caspase9 substrate sequence (LEHD) is after NLS s…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. Quartet-SpyTag

    Plasmid
    #214726
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 each separated by GS-linkers with a C-terminal SpyTag fusion
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    Quartet-SpyTag (S Synthetic)
    Use
    Tags
    SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214726-plasmid-map-sequence-id-435531

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  17. SpyTag-Quartet

    Plasmid
    #214727
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 each separated by GS-linkers with an N-terminal SpyTag fusion
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Quartet (S Synthetic)
    Use
    Tags
    SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214727-plasmid-map-sequence-id-435532

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  18. SpyTag-Kraken Quartet

    Plasmid
    #214730
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 XBB.1.5 separated by GS-linkers with an N-terminal SpyTag
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Kraken Quartet (S Synthetic)
    Use
    Tags
    SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214730-plasmid-map-sequence-id-435535

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  19. SpyTag-Quartet_NoLinker

    Plasmid
    #214731
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 with no linkers between the RBDs and an N-terminal SpyTag
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Quartet_NoLinker (S Synthetic)
    Use
    Tags
    SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214731-plasmid-map-sequence-id-435536

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Showing: 3961 - 3980 of 5011 results