We narrowed to 58,407 results for: tra

1. pRCPam

   Plasmid

   #195861

   Purpose

   Encodes chromogenic protein (CP) with nonsense mutation resulting in amber stop codon at amino acid 62. CP inducible with rhamnose in amber suppressor strains.

   Depositor

   Gregory Phillips

   Article

   DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

   Insert

   Amber Chromogenic Protein on pRCPam plasmid
   

   Expression

   Bacterial

   Mutation

   Gln62X

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-baPrs-hdNadV

   Plasmid

   #91950

   Purpose

   pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

   Depositor

   George Cătălin Marinescu

   Article

   Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

   Inserts

   Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
   Phosphoribosyl Pyrophosphate Synthetases
   

   Expression

   Bacterial

   Mutation

   changed Leucine 135 to Isoleucine

   Promoter

   T7

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSETC-RdgBbeta-splice variant 1

   Plasmid

   #58276

   Purpose

   bacterial expression of human RdgBbeta splice variant 1

   Depositor

   Shamshad Cockcroft

   Article

   Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

   Insert

   RdgBbeta (PITPNC1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pMSCV-SOCS6-WT

   Plasmid

   #74496

   Purpose

   Overexpression of SOCS6 in mammalian cells

   Depositor

   Lars Rönnstrand

   Article

   Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

   Insert

   SOCS6 (Socs6 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   unknown

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pLPCX-Cx43-FS-IRES-GFP

   Plasmid

   #65438

   Purpose

   Mammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral production

   Depositor

   Trond Aasen

   Article

   Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

   Insert

   Cx43 (GJA1 Human)
   

   Use

   Retroviral

   Tags

   IRES-GFP

   Expression

   Mammalian

   Mutation

   frameshift (FS) mutation 35delA

   Promoter

   CMV

   Available Since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMW76: pET-His-MBP-SUMO-R2Bm-33XTEN- SpCas9(H840A)-SII

   Plasmid

   #209292

   Purpose

   Expresses R2Bm-Cas9(H840A) fusion in E. coli

   Depositor

   Feng Zhang

   Article

   Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

   Insert

   R2
   

   Tags

   His, MBP, SpCas9(H840A), StrepII, and bdSUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAR-9.47_VP1,3

   Plasmid

   #74240

   Purpose

   Original plasmid described in Choudhury et al. 2015 Mol Ther. for production of AAV-AS vectors. Encodes VP1 and VP3 of AAV9.47

   Depositor

   Miguel Sena-Esteves

   Article

   Choudhury et al Mol Ther. 2015 Dec 28. doi: 10.1038/mt.2

   Inserts

   AAV2 Rep
   AAV9.47 VP1
   AAV9.47 VP3
   

   Use

   AAV

   Available Since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCHOP10(dLZ).pCDNA1

   Plasmid

   #21900

   Depositor

   David Ron

   Article

   Ron et al Genes Dev. 1992 Mar;6(3):439-53. doi: 10

   Insert

   CHOP10 (Ddit3 Mouse)
   

   Expression

   Mammalian

   Mutation

   The coding region of the murine CHOP-10 cDNA was …

   Available Since

   Oct. 8, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pKS7107

   Plasmid

   #89051

   Purpose

   Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   Nm crRNA
   Nm tracrRNA
   hNmCas9
   

   Use

   CRISPR

   Tags

   HA tag, NLS, and SV40 NLS

   Promoter

   EF1a and human U6

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB115 - pL2_pSB90_fLUC-I_tGFP-I

    Plasmid

    #123190

    Purpose

    binary plant vector for transient tGFP (with intron) and fLUC (with intron) expression

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    2x35S::tGFP-I::tNOS 2x35S::fLUC-I::tNOS
    

    Use

    Luciferase

    Expression

    Plant

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. MO70 - Orai1 EC-Flag

    Plasmid

    #79590

    Purpose

    Transient expression and retroviral Expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al J Biol Chem. 2010 Feb 12;285(7):5066-75.

    Insert

    Orai1 (ORAI1 Human)
    

    Use

    Retroviral

    Tags

    FLAG (inserted between TM3 and TM4 of the protein…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

12. TFORF1304

    Plasmid

    #144293

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZMIZ2 (ZMIZ2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. NET50 pmRFP-N2 (489)

    Plasmid

    #61983

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET50 (DHRS7 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

14. NET97 pmRFP-N2 (641)

    Plasmid

    #61994

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    AADACL1 (NCEH1 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS

    Plasmid

    #123188

    Purpose

    binary plant vector for transient fLUC (with intron) expression

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone
    

    Use

    Luciferase

    Expression

    Plant

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCLASP_Crz1(19A)_CLASP_RGS2membrane

    Plasmid

    #133085

    Purpose

    Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    Crz1*-CLASP
    

    Expression

    Bacterial and Yeast

    Promoter

    pADH1

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. NET37 pmRFP-N2 (450)

    Plasmid

    #61980

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET37 (KIAA1161 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

18. sTR060

    Plasmid

    #177370

    Purpose

    Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

    Depositor

    Jerome Bonnet, Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Insert

    Trigger RNA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-NES-tdTomato-LINuS-WPRE

    Plasmid

    #159894

    Purpose

    AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

    Insert

    NES-tdTomato-LINuS
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF1α promoter

    Available Since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. TAPBPL pEGFP-C3 (1765)

    Plasmid

    #62055

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al PLoS One. 2011 Apr 14;6(4):e18762. doi:

    Insert

    TAPBPL (TAPBPL Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only