We narrowed to 325 results for: Stil

1. pcDNA5 FRT/TO Plk4 ∆24-mCherry

   Plasmid

   #80271

   Purpose

   mammalian expression plasmid for Plk4 with multi-phospho degron deleted

   Depositor

   Andrew Holland

   Article

   Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

   Insert

   Plk4 (PLK4 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   deletion of multi-phospho degron (amino acids 282…

   Promoter

   CMV

   Available since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

   Plasmid

   #80272

   Purpose

   mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted

   Depositor

   Andrew Holland

   Article

   Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

   Insert

   Plk4 (PLK4 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   deletion of multi-phospho degron (amino acids 282…

   Promoter

   CMV

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR FL CPAP RNAi resistant

   Plasmid

   #46390

   Depositor

   Pierre Gonczy

   Article

   Kitagawa et al J Cell Sci. 2011 Nov 15;124(Pt 22):3884-

   Insert

   CPAP RNAi resistant mutant (CENPJ Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   silent mutation that renders it resistant to siRN…

   Promoter

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCas9_sgRNA_0

   Plasmid

   #70763

   Purpose

   expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating

   Depositor

   Regine Kahmann

   Article

   Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10

   Inserts

   U6 promoter
   cas9
   tnos terminator
   

   Use

   Self-replicating in ustilago maydis, conferring c…

   Tags

   N-terminal NLS, C-terminal HA-tag +NLS

   Expression

   Mutation

   Streptococcus pyogenes gene codon-optimized for U…

   Promoter

   U6 from Ustilago maydis and synthetic otef promot…

   Available since

   Nov. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. p123shy1-mCherry-HA

   Plasmid

   #67875

   Purpose

   encodes shy1 with a C-terminal mCherry-HA tag under control of its endogenous promoter.It's suitable for localization studies in Ustilago maydis.

   Depositor

   Armin Djamei

   Article

   Rabe et al Mol Microbiol. 2013 Jul;89(1):179-88. do

   Insert

   shy1
   

   Use

   Expression in ustilago maydis

   Tags

   HA and mCherry

   Expression

   Mutation

   Promoter

   shy1

   Available since

   Aug. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMS73

   Plasmid

   #110629

   Purpose

   CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating

   Depositor

   Regine Kahmann

   Article

   Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d

   Inserts

   short U6 promoter
   cas9
   tnos terminator
   

   Use

   CRISPR; Self-replicating in ustilago maydis, conf…

   Tags

   N-terminal NLS, C-terminal HA-tag +NLS

   Expression

   Bacterial

   Mutation

   Streptococcus pyogenes gene codon-optimized for U…

   Promoter

   U. maydis hsp70 promoter (Kronstad and Leong, 198…

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. HO-Poly-HO

   Plasmid

   #51661

   Purpose

   yeast plasmid for integration of target sequence at HO locus

   Depositor

   David Stillman

   Article

   Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

   Type

   Empty backbone

   Use

   Yeast integration

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC21-NotI

   Plasmid

   #51659

   Purpose

   Bacterial cloning vector based on pUC21 (Amp)

   Depositor

   David Stillman

   Article

   Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUK21-NotI

   Plasmid

   #51660

   Purpose

   Bacterial cloning vector based on pUK21 (Kan)

   Depositor

   David Stillman

   Article

   Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. HO-Poly-KanMX4-HO

    Plasmid

    #51662

    Purpose

    yeast plasmid for integration of target sequence at HO locus, Kan selection

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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