1. mEmerald-LaminA-C-18

   Plasmid

   #54138

   Purpose

   Localization: Nuclear Envelope, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   LaminA (LMNA Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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1. pUC19_GNB1L_dTAG

   Plasmid

   #224378

   Purpose

   Generates GNB1L dTAG C terminal knock-in cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   GNB1L (GNB1L Human)
   

   Use

   Tags

   dTAG

   Expression

   Bacterial

   Mutation

   Synonymous mutation

   Promoter

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-22HT-MSI1(7-192)R53E/R59E/R61E

   Plasmid

   #60355

   Purpose

   R53E R59E R61E triple mutant of the mouse MSI1 RBD

   Depositor

   Sean Ryder

   Article

   Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

   Insert

   mouse MSI1 (7-192) (Msi1 Mouse)
   

   Use

   Tags

   His and TEV protease site

   Expression

   Bacterial

   Mutation

   R53E, R59E and R61E mutations; contains amino aci…

   Promoter

   T7

   Available since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pLenti_CMV_Puro_SFB_GNB1L

   Plasmid

   #224381

   Purpose

   Lenti plasmid for generating GNB1L expressing stable cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   GNB1L (GNB1L Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX459_GNB1L_gRNA

   Plasmid

   #224377

   Purpose

   Targets GNB1L for dTAG C terminal knock-in cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   GNB1L (GNB1L Human)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti_CMV_Puro_SFB_GNB1L_WD 7 deletion

   Plasmid

   #224388

   Purpose

   Lenti plasmid for generating GNB1L _WD 7 domain depleted expressing stable cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   GNB1L (GNB1L Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   WD 7 domain deleted (aa 286-323)

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion

   Plasmid

   #224387

   Purpose

   Lenti plasmid for generating GNB1L _WD 6 domain depleted expressing stable cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   GNB1L (GNB1L Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   WD 6 domain deleted (aa 242-282)

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_CMV_Puro_SFB_GNB1L_WD 4 deletion

   Plasmid

   #224385

   Purpose

   Lenti plasmid for generating GNB1L _WD 4 domain depleted expressing stable cell lines

   Depositor

   Junjie Chen

   Article

   Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

   Insert

   GNB1L (GNB1L Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   WD 4 domain deleted (aa 153-195)

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-FRT/T0-EGFP-GNB1L

   Plasmid

   #222621

   Purpose

   Mammalian expression vector of flag-tagged GNB1L

   Depositor

   Daniel Durocher

   Article

   Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

   Insert

   GNB1L (GNB1L Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pET28 E2 LBD-Tev-E1a-His

   Plasmid

   #232117

   Purpose

   For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.

   Depositor

   Ruth Sommese

   Article

   Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

   Insert

   DBT (DBT Human)
   

   Use

   Tags

   6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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