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pWS5394-CRISPRai-MET17

Plasmid

#182833

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - MET17 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Met1 diUb (1-152)

Plasmid

#110766

Purpose

Bacterial expression of Met1 diUb

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

UBB (UBB Human)

Use

Tags

N-His6-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-MET17

Plasmid

#122889

Purpose

LYS2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-MET17

Plasmid

#122906

Purpose

ADE2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMET17p-CCG 316

Plasmid

#131166

Purpose

For ScMET17 promoter regulated expression of mCherry-Cub-R-GFP (CCG) fused bait proteins, centromeric ARS plasmid, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2015;1346:151-68. doi:

Type

Empty backbone

Use

Tags

CCG

Expression

Yeast

Mutation

Promoter

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-MET17

Plasmid

#122885

Purpose

ADE2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE TRP1-MET17

Plasmid

#122893

Purpose

TRP1-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp LYS2-MET17

Plasmid

#122910

Purpose

LYS2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp TRP1-MET17

Plasmid

#122914

Purpose

TRP1-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
P1K-3HA-Atg8∆R-MET15

Plasmid

#207034

Purpose

Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Use

Tags

3xHA

Expression

Yeast

Mutation

∆R

Promoter

pATG8

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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P1K-3HA-Atg8∆GR-MET15

Plasmid

#207033

Purpose

Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Use

Tags

3xHA

Expression

Yeast

Mutation

∆GR

Promoter

pATG8

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Met133I, M1381I, M186I ELL2

Plasmid

#127263

Purpose

For in vitro translation of human ELL2 with Met133I, M1381I, M186I

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

Three Mets (133, 138, 186) to Ileu

Promoter

T7

Available since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Met1 diUb (1-152, R42W)

Plasmid

#110767

Purpose

Bacterial expression of Met1 diUb

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

UBB (UBB Human)

Use

Tags

N-His6-3C

Expression

Bacterial

Mutation

R42W

Promoter

T7

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8-MET15

Plasmid

#207055

Purpose

Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Use

Tags

3xVSV

Expression

Yeast

Mutation

Promoter

pATG8

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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289aa Met133, 138, 186Ileu ELL2

Plasmid

#127272

Purpose

Expresses 289aa human ELL2 with Met133, 138, 186Ileu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 w Met to ILeus stopped at XbaI (ELL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Met133, 138, 186 Ileu, cut with XbaI blunt fillin

Promoter

pEF1a

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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ClhN-P1K-3VSV-Atg8(P85L P86L)-MET15

Plasmid

#207050

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Use

Tags

3xVSV

Expression

Yeast

Mutation

P85L P86L

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA tagged Xba ELL2 Met133, 138, 186 to Ileu

Plasmid

#127269

Purpose

For in vitro translation of ELL2 with with internal HA tag and Met133, 138, 186 Ileu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 Met133, 138, 186 to Ileu (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

Met133, 138, 186 to Ileu HA tag after M186

Promoter

T7

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
HA tagged Sph ELL2 Met133, 138, 186 to Ileu

Plasmid

#127271

Purpose

For in vitro translation of human ELL2 with Met133, 138, 186 to Ileu with HA tag after 8th Met

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 Met133, 138, 186 to Ileu (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

Met133, 138, 186 to Ileu with HA tag after 8th Met

Promoter

T7

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-P1K-3VSV-Atg8(T56A R65A T87A)-MET15

Plasmid

#207051

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Use

Tags

3xVSV

Expression

Yeast

Mutation

T56A R65A T87A

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-P1K-3VSV-Atg8(R65A P85A P86A)-MET15

Plasmid

#207052

Purpose

Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Use

Tags

3xVSV

Expression

Yeast

Mutation

R65A P85A P86A

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge