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pLz47_polyPro_CsoS2_Npeptide

Plasmid

#140845

Purpose

Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 N-peptide

Use

Tags

Hexahistidine tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pLz55_polyPro_random_Npeptide

Plasmid

#140846

Purpose

Same as pLz47 but with randomized N*-peptide sequence

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with randomized CsoS2 N-peptide

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pLz56_polyPro_CsoS2_MR_r1

Plasmid

#140848

Purpose

Same as pLz47 but with MR repeat 1 instead of N*-peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 MR repeat 1

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pLz57_polyPro_CsoS2_CTD_r8

Plasmid

#140849

Purpose

Same as pLz47 but with CTD repeat 8 instead of N*-peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 CTD repeat 8

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pLz58_polyPro_CsoS2_CTP

Plasmid

#140850

Purpose

Same as pLz47 but with C-terminal peptide (CTP) instead of N*- peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 C-terminal peptide

Use

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pOlyA(WT)

Plasmid

#132391

Purpose

Ostreolysin A for sensing sphingomyelin/cholesterol complexes

Depositor

Arun Radhakrishnan

Article

Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

Insert

Ostreolysin A wild-type

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: C62S, C94S, S151C

Promoter

Availability

Academic Institutions and Nonprofits only

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PoliIFN-γ

Plasmid

#107289

Purpose

Plasmid can be used for secretion of fish Interferon gamma

Depositor

Bohdan Schneider

Article

Zahradnik et al Fish Shellfish Immunol. 2018 May 6;79:14

Insert

Interferon gamma

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Availability

Academic Institutions and Nonprofits only

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pOLENTE

Plasmid

#80683

Purpose

DEST vector for cell-free in vitro expression

Depositor

Nico Dissmeyer

Type

Empty backbone

Use

Synthetic Biology; In vitro transcription and tra…

Tags

Expression

Mammalian

Mutation

Promoter

T7 and SP6

Availability

Academic Institutions and Nonprofits only

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poLuc1

Plasmid

#154128

Purpose

Expresses orthogonal firefly luciferase gene in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Insert

orthogonal firefly luciferase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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POLArISact

Plasmid

#164971

Purpose

Expresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cells

Depositor

Sumio Terada

Article

Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

Insert

POLArISact

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pDmPolyUbiq

Plasmid

#165786

Purpose

Drosophila Polyubiquitin promoter, strongest promoter in the library

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

PolyUbiq

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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polyPRM5

Plasmid

#175232

Purpose

Bacterial expression and purification, mixed with polySH3 to form condensates

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

ABL1 (ABL1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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pPOLY_new

Plasmid

#179276

Purpose

Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)

Depositor

Bernd Nidetzky

Article

Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

Inserts

cellodextrin phosphorylase
cellobiose phosphorylase
sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)

Use

Synthetic Biology

Tags

6xHIS and Strep-Tag II

Expression

Bacterial

Mutation

Promoter

T7 lacO

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PICK1_POLR2F

Plasmid

#205894

Purpose

Express mEGFP-tagged fusion protein, PICK1_POLR2F from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PICK1_POLR2F (PICK1, POLR2F Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_TTC6_MIPOL1

Plasmid

#205949

Purpose

Express mEGFP-tagged fusion protein, TTC6_MIPOL1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TTC6_MIPOL1 (TTC6, MIPOL1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ZC3H4_POLR2I

Plasmid

#205956

Purpose

Express mEGFP-tagged fusion protein, ZC3H4_POLR2I from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ZC3H4_POLR2I (ZC3H4, POLR2I Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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POLG_Halo_C_allele

Plasmid

#178134

Purpose

Donor vector for endogenous tagging of human POLG at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (POLG Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Cas9_P2A_POLB

Plasmid

#190145

Purpose

Expresses SpCas9 and POLB

Depositor

Timothy Lu

Article

Nakade et al bioRxiv 2022.12.05.518807

Insert

SpCas9_P2A_POLB

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Codon Optimized for mammalian cells

Promoter

CBh

Availability

Academic Institutions and Nonprofits only

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Cas9_P2A_T4pol

Plasmid

#190146

Purpose

Expresses SpCas9 and T4pol

Depositor

Timothy Lu

Article

Nakade et al bioRxiv 2022.12.05.518807

Insert

SpCas9_P2A_T4pol

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Codon Optimized for mammalian cells

Promoter

CBh

Availability

Academic Institutions and Nonprofits only

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pT7CFE1_polio_3ABC_C147R

Plasmid

#212985

Purpose

Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts

Depositor

Karla Kirkegaard

Insert

Poliovirus Type I Mahoney 3ABC C147R

Use

Mammalian cell free protein expression

Tags

HA

Expression

Mutation

cysteine 147 in 3C changed to arginine

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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