We narrowed to 178 results for: SSB

1. 6974 pPROEX-HTb-SSB1

   Plasmid

   #1232

   Depositor

   Susan Lindquist

   Article

   Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

   Insert

   ssb1 (SSB1 Budding Yeast)
   

   Tags

   his tag (tev cleavable)

   Expression

   Bacterial

   Available Since

   Aug. 9, 2005

   Availability

   Academic Institutions and Nonprofits only

2. KHBD00488

   Plasmid

   #39593

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG8396 (Ssb-c31a Fly)
   

   Use

   Gateway donr vector

   Promoter

   none

   Available Since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

3. SPSB4

   Plasmid

   #38896

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SPSB4 (SPSB4 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. SPSB1

   Plasmid

   #38981

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SPSB1 (SPSB1 Human)
   

   Tags

   6xHis-Tev

   Expression

   Bacterial

   Mutation

   Residues 24-233 of SPSB1 are present in this plas…

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKD077

   Plasmid

   #136473

   Purpose

   SSB-mTur2 IDL fusion expression plasmid

   Depositor

   James Keck

   Article

   Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

   Insert

   SSB-mTur2 (ssb E. coli)
   

   Tags

   mTurquoise2

   Expression

   Bacterial

   Mutation

   mTurquoise2 inserted between Phe148 and Ser149

   Promoter

   T7 promoter

   Available Since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKD068

   Plasmid

   #136471

   Purpose

   SSB-GFP IDL fusion expression plasmid

   Depositor

   James Keck

   Article

   Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

   Insert

   SSB-GFP (ssb E. coli)
   

   Tags

   superfolder GFP

   Expression

   Bacterial

   Mutation

   superfolder GFP inserted between Phe148 and Ser149

   Promoter

   T7 promoter

   Available Since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKD072

   Plasmid

   #136472

   Purpose

   SSB-C-term-GFP fusion expression plasmid

   Depositor

   James Keck

   Article

   Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

   Insert

   SSB-C-term-GFP (ssb E. coli)
   

   Tags

   superfolder GFP

   Expression

   Bacterial

   Mutation

   superfolder GFP attached to C-terminus at Phe 178

   Promoter

   T7 promoter

   Available Since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. ALARM NMR

   Plasmid

   #90209

   Purpose

   Expresses the human La antigen for use in ALARM NMR

   Depositor

   Michael Walters

   Article

   Dahlin et al Nat Commun. 2017 Nov 15;8(1):1527. doi:

   Insert

   La antigen (SSB Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   contains amino acids 100-324

   Promoter

   T7 promoter

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-PE7-mutant (Q20A_Y23A_Y24F_F35A)

   Plasmid

   #224123

   Purpose

   Mammalian expression of SpCas9 PE7 mutant prime editor (Q20A_Y23A_Y24F_F35A)

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-mutant (Q20A_Y23A_Y24F_F35A)
   

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Q20A_Y23A_Y24F_F35A in La(SSB)1-194

   Promoter

   CMV

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCF.111

    Plasmid

    #227187

    Purpose

    pORTMAGE-Ec1 + supplement E. coli SSB

    Depositor

    Seth Shipman

    Article

    Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

    Insert

    CspRecT, mutL_E32K, E. coli SSB
    

    Expression

    Bacterial

    Mutation

    mutL E32K

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-PE7

    Plasmid

    #214812

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-PE7 for IVT

    Plasmid

    #214813

    Purpose

    Template for in vitro transcription of PE7

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Promoter

    T7 (inactivated)

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. T7_CC_PE7_IVT_Template

    Plasmid

    #223022

    Purpose

    Template for in vitro transcription of PE7. For HSPC-related experiments.

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Template for in vitro transcription

    Tags

    SV40 bpNLS and c-Myc NLS

    Promoter

    T7

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. SPSB2

    Plasmid

    #38983

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SPSB2 (SPSB2 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pCMV-PE7-P2A-GFP

    Plasmid

    #222996

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-P2A-EGFP
    

    Tags

    P2A-EGFP, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PE7-P2A-BSD

    Plasmid

    #224122

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-P2A-BSD
    

    Tags

    P2A-BSD, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. PE7_AAVS1_knockin_HDR_donor

    Plasmid

    #223001

    Purpose

    HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-PE7-P2A-hMLH1dn

    Plasmid

    #222997

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-P2A-hMLH1dn
    

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Deletion of residues 754-756 in MLH1 as described…

    Promoter

    CMV

    Available Since

    Aug. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX335-NEAT1_IS_v1

    Plasmid

    #97085

    Purpose

    Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    sgRNA NEAT1_IS_v1
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX335-NEAT1_IS_v2

    Plasmid

    #97086

    Purpose

    Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    sgRNA NEAT1_IS_v2
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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