Showing: 21 - 40 of 1588 results
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Plasmid#41103DepositorInsert8xmiR307_21nt-seedonly
UseLuciferaseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Needle
Plasmid#84868PurposeBaculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Needle
UseBaculovirusTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ (M)-YFP-Eed
Plasmid#82512PurposeLentivirus vector. Expresses YFP-Eed fusion proteins in mammalian cells.DepositorInsertembryonic ectoderm development
UseLentiviralTagsHaloTagExpressionMammalianMutationinsert contains AA39-441 of EeDPromotercmvAvailabilityAcademic Institutions and Nonprofits only -
pAT9650-BEAR-GFP-preedited
Plasmid#162992PurposeBEAR control plasmid with split EGFP and intact 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAT9751-BEAR-mCherry-preedited
Plasmid#162993PurposeBEAR control plasmid with split mCherry and intact 5' splice siteDepositorInsertmCherry split with an intron between amino acids 119-120
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pAT9753-BEAR-mScarlet-preedited
Plasmid#162994PurposeBEAR control plasmid with split mScarlet and intact 5' splice siteDepositorInsertmScarlet split with an intron between amino acids 110-111
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Incoherent feedforward (IFF) plasmid
Plasmid#169748PurposeStandalone IFF circuit plasmid that has the same implementation of the IFF subcircuit in Equalizer-L.DepositorInsertmiR(FF4) target-eGFP-miR(FF4) target-miR(FF4)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2AvailabilityAcademic Institutions and Nonprofits only -
GFP-CLCb (EED/QQN)
Plasmid#47422DepositorInsertCLCb EED/QQN (CLTB Human)
UseTagsEGFPExpressionMammalianMutationEED changed to QQN, in aa20-41 regionPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 1x perfect to seed siRNA
Plasmid#40766PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsertCXCR4 small RNA perfect to seed target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 1x perfect to seed plus 13-16 siRNA
Plasmid#40767PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsertCXCR4 small RNA perfect to seed plus 13-16 target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailabilityAcademic Institutions and Nonprofits only -
A365Y-GFP Donor for iWTr EED
Plasmid#131324PurposeDonor to generate i-WT-r systemDepositorInserti-WT-r.Donor
UseCRISPR and Mouse TargetingTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Y365A-GFP Donor for iMTr EED
Plasmid#131325PurposeDonor to generate i-MT-r systemDepositorInserti-MT-r.Donor
UseCRISPR and Mouse TargetingTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
CMV-FFluc-GPD1L-3'UTR Seed Mut
Plasmid#32490DepositorInsertGPD1L 3'UTR (Gpd1l Mouse)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
CJ40 (let-7 seed mutant perfect site)
Plasmid#17773DepositorInsertlet-7 seed mutant perfect site (let-7 Fly)
UseLuciferase; Drosophila metallothionein gene promo…Tagsrenilla luciferaseExpressionInsectMutationseed mutantPromoterAvailabilityAcademic Institutions and Nonprofits only -
CJ38 (miR-1006 seed mutant perfect site)
Plasmid#17771DepositorInsertmiR-1006 seed mutant perfect site
UseLuciferase; Drosophila metallothionein gene promo…Tagsrenilla luciferaseExpressionInsectMutationseed mutantPromoterAvailabilityAcademic Institutions and Nonprofits only -
CJ39 (miR-1003 seed mutant perfect site)
Plasmid#17772DepositorInsertmiR-1003 seed mutant perfect site
UseLuciferase; Drosophila metallothionein gene promo…Tagsrenilla luciferaseExpressionInsectMutationseed mutantPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEx1-pEF-H2B-mCherry-T2A-rTetR-EED
Plasmid#78101PurposeExpresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.DepositorInsertH2B-mCherry-T2A-rTetR-EED (EED Synthetic, Human)
UseSynthetic BiologyTagsrTetRExpressionMammalianMutationPromoterpEF alphaAvailabilityAcademic Institutions and Nonprofits only -
pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES
Plasmid#200402PurposeEntry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detectionDepositorInsertClosed-loop DART VADAR sensor entry cassette
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES
Plasmid#200403PurposeEntry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detectionDepositorInsertClosed-loop DART VADAR sensor entry cassette
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Showing: 21 - 40 of 1588 results