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Luke-2

Plasmid

#228900

Purpose

Luke-2 localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFP

Depositor

John Samuelson

Article

Kanakapura Sundararaj et al mSphere. 2024 Sep 25;9(9):e0046624. doi:

Insert

Carbohydrate binding domain cbm49 protein

Use

Acanthamoeba expression vector

Tags

eGFP

Expression

Mutation

none

Promoter

Own

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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Jonah-1

Plasmid

#228899

Purpose

Jonah-1 localizes to the outer cyst wall of Acanthamoeba tagged with eGFP

Depositor

John Samuelson

Article

Kanakapura Sundararaj et al mSphere. 2024 Sep 25;9(9):e0046624. doi:

Insert

Jonah-1

Use

Acanthamoeba expression vector

Tags

eGFP

Expression

Mutation

none

Promoter

Own promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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Leo-A

Plasmid

#228901

Purpose

Leo-A localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFP

Depositor

John Samuelson

Article

Kanakapura Sundararaj et al mSphere. 2024 Sep 25;9(9):e0046624. doi:

Insert

Leo-A

Use

Acanthamoeba expression vector

Tags

eGFP

Expression

Mutation

none

Promoter

Own

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pSP64-GFP-ePDZ-CAAX

Plasmid

#135603

Purpose

Expression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

Lifeact-GFP-ePDZ-CAAX

Use

In vitro transcription (mrna synthesis)

Tags

CAAX, EGFP, and ePDZ

Expression

Mutation

Promoter

Unknown

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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SpMTA Purple Sea Urchin Metallothionein

Plasmid

#200292

Purpose

Bacterial expression plasmids for production of recombinant Purple Sea Urchin Strongylocentrotus purpuratus Metallothionein

Depositor

Artur Krężel

Article

Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

Insert

Gene of metallothionein with Chiting Binding Domanin-Intein

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pG.Lantern-SM50-GFP

Plasmid

#135593

Purpose

Expression of SM50 Promoter-GFP mRNA for microinjection to visualize SM50 promoter activity in S. purpuratus

Depositor

Mamiko Yajima, Koji Akasaka

Article

Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

Insert

SM50 promoter (SM50 S. purpuratus)

Use

In vitro transcription (mrna synthesis)

Tags

Green Lantern GFP

Expression

Mutation

Promoter

CMV

Available since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pEMB52 / Urchin_LRRK2_1_335

Plasmid

#40551

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2

Use

Baculovirus

Tags

6xHis

Expression

Mutation

WT

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pEMB403 Urchin_LRRK2_1_335

Plasmid

#40561

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2

Use

Baculovirus

Tags

6xHis and GST

Expression

Mutation

WT

Promoter

polH

Available since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
KRP95

Plasmid

#24447

Depositor

Ron Vale

Article

Pierce et al Biochemistry. 1999 Apr 27. 38(17):5412-2

Insert

Kinesin II (KRP95 Strongylocentrotus purpuratus)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pCS2+ABCB1a

Plasmid

#38036

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCB1a

Use

Sea urchin, zebrafish, xenopus

Tags

Expression

Mutation

Promoter

SP6

Available since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only
Kaede-SpVasa

Plasmid

#185582

Purpose

for photoconversion

Depositor

Mamiko Yajima

Article

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

Vasa

Use

In vitro transcription (mrna synthesis)

Tags

Kaede

Expression

Mutation

Promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+8CmCherry-ABCC1

Plasmid

#40587

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCC1

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mutation

Promoter

SP6

Available since

Jan. 16, 2013

Availability

Academic Institutions and Nonprofits only
Dendra-SpVasa

Plasmid

#185583

Purpose

for photoconversion

Depositor

Mamiko Yajima

Article

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

Vssa

Use

In vitro transcription (mrna synthesis)

Tags

Dendra

Expression

Mutation

Promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-SpGalpha-i

Plasmid

#185568

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

Galpha-i

Use

In vitro transcription (mrna synthesis)

Tags

GFP, S-Tag

Expression

Mutation

Promoter

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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SpPLK1-GFP

Plasmid

#185573

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Expression

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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SpPLK1L-GFP

Plasmid

#185574

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

PLK1L

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Expression

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+8CmCherry-ABCC9a

Plasmid

#35208

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCC9a

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+CeGFP-ABCC9a

Plasmid

#35581

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCC9a

Use

Sea urchin, zebrafish, xenopus

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+8NmCherry-ABCC5a

Plasmid

#35207

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCC5a

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
pCS2+8NCerulean-ABCB1a

Plasmid

#34944

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCB1a

Use

Sea urchin, zebrafish, xenopus

Tags

Cerulean

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only