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pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA

Plasmid

#184549

Purpose

Retroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tag

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Inserts

Malate dehydogenase 1 (MDH1 Human)
Malic Enzyme 1 (ME1 Human)

Use

Retroviral

Tags

3xFLAG and HA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pUltra-MDH1-ME1

Plasmid

#184465

Purpose

Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Inserts

Malate dehydrogenase 1 (MDH1 Human)
Malic Enzyme 1 (ME1 Human)

Use

Lentiviral

Tags

fused to T2A

Expression

Mammalian

Mutation

deletion of stop codon

Promoter

UbC

Available since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAM299

Plasmid

#224258

Purpose

MCMUbHis

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

MC-Ub-3C-His

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAM301

Plasmid

#226134

Purpose

Expression of MC-Ub-SH3-3C-His6

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

MC-Ub-SH3-3C-His6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM298

Plasmid

#224257

Purpose

MCM WT ubiquitin

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

MCM Ubiquitin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAM305

Plasmid

#226137

Purpose

Expression of His6-mEos3.2-UbK48R

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-mEos3.2-UbK48R

Use

Tags

Expression

Bacterial

Mutation

K48R

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAM302

Plasmid

#226135

Purpose

Expression of MC-Ub-UbK48R-SH3-3C-His6

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

MC-Ub-UbK48R-SH3-3C-His6

Use

Tags

Expression

Bacterial

Mutation

K48R

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAM303

Plasmid

#226136

Purpose

Expression of His6-GG-UbK48R

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-GG-UbK48R

Use

Tags

Expression

Bacterial

Mutation

K48R

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM294

Plasmid

#226130

Purpose

Expression of Ufd1-3C-His6

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Ufd1-3C-His6

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM282

Plasmid

#226121

Purpose

Expression of cdc48

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-3C-Cdc48 (CDC48 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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