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Plasmid#87648PurposeFor creating drosophila transgenics expressing UAS-Syt::Halo7DepositorInsertSyt::Halo7
UseTagsExpressionInsectMutationS265G in Syt (please see depositor's commen…PromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
ULK1-Halo HRD
Plasmid#207548PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the C-terminus of the endogenous ULK1 locus in human cellsDepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human ULK1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFN18A-HaloTag-SpyCatcher
Plasmid#206041PurposeHaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTagDepositorTypeEmpty backboneUseTagsHaloTag and SpyCatcher-8HisExpressionBacterialMutationPromoterT7Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lifeact_HaloTag9
Plasmid#175545PurposeExpression of HaloTag9 at the actin filaments of mammalian cellsDepositorInsertLifeact-HaloTag9
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEMS-Halo7Tag-hFis
Plasmid#111136PurposehFis with selflabeling tag, e.g. for fluorescence microscopyDepositorInserthuman fission factor1 (FIS1 Human)
UseTagsHalo7-tagExpressionMammalianMutation*PromoterCMVAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-HaloTag-PuroR
Plasmid#80960PurposeCRISPaint gene taggingDepositorInsertHaloTag
UseGene taggingTagsExpressionMutationPromoternoneAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHTC-HaloTag_RAB7A:1-207
Plasmid#211096PurposeMammalian expression of full-length human RAB7A. C-terminal HaloTag.DepositorInsertRAB7A:M1-C207 (RAB7A Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
RNF168-Halo HRD
Plasmid#207080PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.DepositorInsertHaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
UseTagsExpressionMammalianMutationPromoterNoneAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-HaloTag
Plasmid#188907PurposeExpresses synaptic HaloTagDepositorInsertSYP (Syp Mouse)
UseLentiviralTagsHaloTagExpressionMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-yoHaloTag-TLG1
Plasmid#105277PurposePlasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.DepositorInsertTlg1 (TLG1 Budding Yeast)
UseTagsyoHaloTagExpressionYeastMutationHaloTag has yeast optimized codonsPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEMS-Tom20-Halo7Tag
Plasmid#111135PurposeTom20 with selflabeling tag, e.g. for fluorescence microscopyDepositorInsertReceptor of tranlocase of outer mitochondrial membrane Tom20 (TOMM20 Human)
UseTagsHalo7 TagExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHTN-HaloTag_BCL11A:1-773
Plasmid#211014PurposeMammalian expression of full-length human BCL11A. N-terminal HaloTag.DepositorInsertBCL11A:M1-F773 (BCL11A Human)
UseTagsHaloTagExpressionMammalianMutationisoform 2PromoterCMVAvailable sinceFeb. 21, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Halo-WIPI2 HRD
Plasmid#207544PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous WIPI2 locus in human cellsDepositorInsertHaloTag flanked by human WIPI2 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHTN-HaloTag_DDB1:1-1140
Plasmid#211006PurposeMammalian expression of full-length human DDB1. N-terminal HaloTag.DepositorInsertDDB1:M1-H1140 (DDB1 Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Halo-TNFa-RUSH
Plasmid#166901PurposeRUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-HaloDepositorInsertHaloTag
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha-HaloTag
Plasmid#179715PurposeLentiviral expression of mouse Syt7 with C terminal HaloTagDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
TS246_pNKI-2-13_HALO-DPN7
Plasmid#200720Purposeproducing Methyl Tracer HALO protein in bacteriaDepositorInsertHALO-DPN7
UseTags6xHisExpressionBacterialMutationcodon optimizedPromoterAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHTC-HaloTag_DDB1:1-1140
Plasmid#211007PurposeMammalian expression of full-length human DDB1. C-terminal HaloTag.DepositorInsertDDB1:M1-H1140 (DDB1 Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb
Plasmid#175534PurposeExpression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag7-myc_PDGFRtmb
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-TfR-RUSH
Plasmid#166905PurposeRUSH cargo: Str-KDEL_IRES_Transferrin Receptor (TfR)-SBP-HaloDepositorInsertHaloTag
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only