We narrowed to 22,696 results for: Sis;

1. Lenti-EFS-PEmax-P2A-Puro

   Plasmid

   #228890

   Purpose

   Lentiviral delivery and expression of PEmax protein with puromycin selection cassette.

   Depositor

   Francisco J Sánchez-Rivera

   Article

   Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

   Inserts

   PEmax
   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTALdNC-AAVS1_T1

   Plasmid

   #80495

   Purpose

   AAVS1 TALEN expression vector (Left)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   AAVS1 TALEN (Left)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   Promoter

   none

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTALdNC-AAVS1_T2

   Plasmid

   #80496

   Purpose

   AAVS1 TALEN expression vector (Right)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   AAVS1 TALEN (Right)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   Promoter

   none

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVS1-P-CAG-DEST

   Plasmid

   #80490

   Purpose

   Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAVS1-P-MCS

   Plasmid

   #80488

   Purpose

   donor vector for AAVS1 targeting (puromycin selection) with multiple cloning site

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-GINKO1

   Plasmid

   #113111

   Purpose

   Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   GINKO1
   

   Use

   Tags

   6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRCT

   Plasmid

   #60621

   Purpose

   Plasmid encoding iCas9, tracrRNA and crRNAs

   Depositor

   Huimin Zhao

   Article

   Bao et al ACS Synth Biol. 2014 Sep 19.

   Inserts

   iCas9
   tracrRNA
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Yeast

   Mutation

   changed Aspartate 147 to Tyrosine, Proline 411 to…

   Promoter

   RPR1p and TEF1p

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC1-SypHer3s

   Plasmid

   #108118

   Purpose

   Ratiometric pH probe with enhanced brightness for mammalian cells expression.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

   Insert

   SypHer3s
   

   Use

   Tags

   ratiometric YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAVS1-Nst-MCS

   Plasmid

   #80487

   Purpose

   donor vector for AAVS1 targeting (neomycin selection) with multiple cloning site

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Type

   Empty backbone

   Use

   Donor vector (human)

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX6P-1-eIF4E K119A

    Plasmid

    #199440

    Purpose

    Bacterial expression plasmids for production of recombinant GST-eIF4E K119A

    Depositor

    Shin-ichi Hoshino

    Article

    Ogami et al STAR Protoc. 2023 May 26;4(2):102340. do

    Insert

    1977 (EIF4E Human)
    

    Use

    Tags

    GST-fusion protein

    Expression

    Bacterial

    Mutation

    changed lysine 119 to alanine

    Promoter

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYESir2HY-puro

    Plasmid

    #1770

    Depositor

    Bob Weinberg

    Article

    Vaziri et al Cell 2001 Oct 19;107(2):149-59.

    Insert

    Sir2 (SIRT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    H363Y, residue 363 changed from histidine to tyro…

    Promoter

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMMB67EH-Spy

    Plasmid

    #90102

    Purpose

    IPTG-inducible CFP/YFP FRET-based biosensor for cyclic-di-GMP measurement using YcgR binding site.

    Depositor

    Samuel Miller

    Article

    Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

    Insert

    YcgR (ycgR )
    

    Use

    Broad-host range

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYFP-NEAT1pr_v2-RT

    Plasmid

    #97088

    Purpose

    Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v2 vector, but also works with px330-NEAT1pr_v1.

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    NEAT1pr_v2-RT (NEAT1 Human)
    

    Use

    CRISPR

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYFP-NEAT1m_v1-RT

    Plasmid

    #97089

    Purpose

    Encodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    NEAT1m_v1-RT (NEAT1 Human)
    

    Use

    CRISPR

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYFP-NEAT1_IS_RT

    Plasmid

    #97091

    Purpose

    Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    NEAT1_IS_RT (NEAT1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX-EGFP-g1

    Plasmid

    #107273

    Purpose

    eGFP sgRNA-1 and Cas9 expression vector (aka. pX-ps1)

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    GFP sgRNA-1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK-flag-USP19 1-1290

    Plasmid

    #78579

    Purpose

    Expression flag-USP19 1-1290 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 1-1290 (USP19 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    mutate 1291 residue to stop condon

    Promoter

    CMV

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pUC57-GFP-SFPQ-RT

    Plasmid

    #97090

    Purpose

    Encodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    EGFP-SFPQ-RT (SFPQ Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCT

    Plasmid

    #60620

    Purpose

    Plasmid encoding iCas9 and tracrRNA on pRS415 backbone

    Depositor

    Huimin Zhao

    Article

    Bao et al ACS Synth Biol. 2014 Sep 19.

    Inserts

    iCas9
    tracrRNA
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Yeast

    Mutation

    changed Aspartate 147 to Tyrosine, Proline 411 to…

    Promoter

    RPR1p and TEF1p

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK-flag-USP19 1-493

    Plasmid

    #78581

    Purpose

    Expression flag-USP19 1-493 in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 1-493 (USP19 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    mutated 494 residue to stop codon

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only