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Plasmid#113513PurposeExpression of His-I3 in insect cells (Baculovirus system)DepositorInsertI3 (PPP1R11 Human)
UseTags6*HisExpressionInsectMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
cmv 16 E7 68CVQ
Plasmid#13691DepositorInsertHPV 16 E7 CVQ6870AAA (E7 Human)
UseTagsExpressionMammalianMutationaa 68-70 changed from CVQ to AAAPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFL His-p97
Plasmid#113510PurposeExpression of His-p97 in insect cells (Baculovirus system)DepositorInsertp97 (VCP Human)
UseTags6*HisExpressionInsectMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCC335
Plasmid#110166PurposeminiMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)DepositorInsertERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B
UseMinimos transposonTagsmCherry and mCloverExpressionWormMutationPhospho-acceptor sites mutated: S43A, T55A, T62A.…Promoterlin-31 promoter/enhancerAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37 SHPmut
Plasmid#113503PurposeBacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP boxDepositorInsertp37 (Ubxn2b Mouse)
UseTagsGST-PreScission protease siteExpressionBacterialMutationF215A/E218A/QKL220-222AAAPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37 deltaUBX
Plasmid#113504PurposeBacterial expression of GST-tagged p37 lacking the UBX domainDepositorInsertp37 (Ubxn2b Mouse)
UseTagsGST-PreScission protease siteExpressionBacterialMutationdelta 251-331 (stop codon behind AA250)PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D
Plasmid#175051PurposeOverexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coliDepositorUseTagsExpressionBacterialMutationchanged Thr76 to Asp, phosphomimetic mutationPromoterT7 promoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA
Plasmid#175050PurposeOverexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coliDepositorUseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tet-off TauRD (P301L/V337M)
Plasmid#188572PurposeDox-repressible expression of the Tau repeat domainDepositorInsertTau repeat domain (MAPT Human)
UseLentiviralTagsMycExpressionMammalianMutationP301L and V337MPromoterpTight TREAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-off Full length Tau (0N4R, P301L/V337M)
Plasmid#188573PurposeDox-repressible expression of the 0N4R Tau isoformDepositorInsert0N4R Tau (MAPT Human)
UseLentiviralTagsExpressionMammalianMutationP301L and V337MPromoterpTight TREAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
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FUGW-APPwt-T2A-mCherry
Plasmid#190803PurposeExpress wild-type APP from Ubiquitin C promoter, co-expressing mCherryDepositorInsertAPP (APP Human)
UseLentiviralTagsExpressionMutationPromoterUbi CAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APPIce-T2A-mCherry
Plasmid#190806PurposeExpress Icelandic mutant APP from Ubiquitin C promoter, co-expressing mCherryDepositorInsertAPP (APP Human)
UseLentiviralTagsExpressionMutationIcelandic (A673T)PromoterUbi CAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
APP_Halo_C_allele
Plasmid#178137PurposeDonor vector for endogenous tagging of human APP at the C-terminus with halotagInsertHalotag (APP Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APPSwe-T2A-Hygro
Plasmid#190805PurposeExpress Swedish mutant APP from Ubiquitin C promoter, co-expressing Hygro resistance geneDepositorInsertAPP (APP Human)
UseLentiviralTagsExpressionMutationSwedish (KM670/671NL)PromoterUbi CAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(MC1-40)
Plasmid#127152PurposeExpresses N-terminal cysteine Abeta(1-40)DepositorInsertAbeta(MC1-40) (APP Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pc DNA FRT TO- APP695
Plasmid#114193PurposeCreation of a stable cell line expressing APP mutation (AD in vitro study)DepositorInsertAmyloid-Precursor-Protein (APP Human)
UseTagsExpressionMammalianMutationwild type splice variant 695: 695AA (2085bp, lack…PromoterCMVAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-APP-CTF
Plasmid#181732PurposeGalactose inducible expression of APP-CTFDepositorInsertAPP-CTF (APP Human)
UseTagsExpressionYeastMutationC-terminal fragment (aa 671-770)PromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pc DNA FRT TO- APPSwed/Ind
Plasmid#114194PurposeCreation of a stable cell line expressing APP mutation (AD in vitro study)DepositorInsertAmyloid-Precursor-Protein (APP Human)
UseTagsExpressionMammalianMutationPopular mouse model, overexpress 2 mutations: Swe…PromoterCMVAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Kir2.1Mut-T2A-tdTomato
Plasmid#60644PurposeExpresses Kir2.1Mut-T2A-tdTomatoDepositorInsertKcnj2 (Kcnj2 Mouse)
UseTagsmycExpressionMammalianMutationGYG to AAA (aa144-146)PromoterAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only