We narrowed to 1,598 results for: pfas

1. pFast-dCry-V5His

   Plasmid

   #136240

   Purpose

   Protein expression of D. melanogaster (Dm) cytochrome with C-terminal V5 and His tags

   Depositor

   Aziz Sancar

   Article

   Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

   Insert

   DmCry
   

   Use

   Tags

   His and V5

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac-GST-Mps1-KD

   Plasmid

   #114066

   Purpose

   protein production

   Depositor

   Geert Kops

   Article

   Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

   Insert

   Mps1-KD (TTK Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Mps1 KD (D664A)

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac-GST-Mps1-WT

   Plasmid

   #114065

   Purpose

   protein production

   Depositor

   Geert Kops

   Article

   Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

   Insert

   Mps1-WT (TTK Human)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   none

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-hphMX6-3nmt1

   Plasmid

   #105162

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

   Insert

   hphMX6-3nmt1
   

   Use

   Tags

   3nmt1

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   3nmt1

   Available since

   Feb. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Plekhm1 - C terminal pFastHFTb

   Plasmid

   #73829

   Purpose

   Express human Plekhm1 C-part in insect cells

   Depositor

   Paul Odgren

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Insect

   Mutation

   cloned C-terminal part of human Plekhm1 (aa 493- …

   Promoter

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

6. Plekhm1-N terminal pFastHFTb

   Plasmid

   #73828

   Purpose

   Express human Plekhm1 N-part in insect cells

   Depositor

   Paul Odgren

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Insect

   Mutation

   cloned N- terminal part of human Plekhm1 (aa 1- 4…

   Promoter

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pFastBac-Gm129-Flag

   Plasmid

   #53766

   Purpose

   baculovirus expression of Gm129

   Depositor

   Aziz Sancar

   Article

   Annayev et al J Biol Chem. 2014 Feb 21;289(8):5013-24.

   Insert

   Gm129 (Ciart Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pFA6a-link-ymNeongreen-URA3

   Plasmid

   #168059

   Purpose

   Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeonGreen
   

   Use

   Tags

   ymNeonGreen

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFA6a-link-yomTagBFP2-Kan

   Plasmid

   #44899

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized mTagBFP2 with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yomTagBFP2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-kanMX-tetO-Pcyc1

    Plasmid

    #41023

    Depositor

    Michael Nick Boddy

    Article

    Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

    Insert

    tetO7-Pcyc1
    

    Use

    Promoter replacement

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA6a-6xGLY-Myc-kanMX6

    Plasmid

    #20769

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFA6a-6xGLY-FLAG-HIS3MX6

    Plasmid

    #20750

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFA6a-link-yomRuby2-Kan

    Plasmid

    #44953

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-link-yoEGFP-Kan

    Plasmid

    #44900

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized EGFP with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBac Dual α1B/β3 Tubulin

    Plasmid

    #170552

    Purpose

    Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB3 (TUBB3 Human)
    

    Use

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-link-yoTagRFP-T-Kan

    Plasmid

    #44906

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoTagRFP-T
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-TID-HA3-HIS3MX6

    Plasmid

    #227981

    Purpose

    To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-kanMX6-P3nmt1-GFP

    Plasmid

    #39289

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    -1163 to +6 of nmt1 locus

    Promoter

    Available since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-6xGLY-3xFLAG-hphMX4

    Plasmid

    #20755

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFA6a-GFP(S65T)-His3MX6

    Plasmid

    #41598

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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