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pBAD-HaloGFP-Na2.4

Plasmid

#228470

Purpose

Bacterial expression of green fluorescent sodium ion indicator HaloGFP-Na2.4

Depositor

Robert Campbell

Article

Takeuchi et al RSC Chem Biol. 2024 Dec 3. doi: 10.1039/

Insert

HaloGFP-Na2.4

Expression

Bacterial

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pscALPS_puro

Plasmid

#128504

Purpose

Encodes puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

Puromycin Resistance

Use

Lentiviral

Expression

Mammalian

Promoter

Cyclophilin A promoter

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-HsSmg7_H

Plasmid

#146476

Purpose

Mammalian Expression of HsSmg7

Depositor

Elisa Izaurralde

Article

Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

Insert

HsSmg7 (SMG7 Human)

Expression

Mammalian

Mutation

one non silent mutation G2560A (V854I) and three …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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p415GPD-3xHA-GW

Plasmid

#63781

Purpose

yeast expression vector p415GPD modified such that it is Gateway converted and introduces a 3xHA tag at the N-terminus of the insert

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Type

Empty backbone

Tags

3xHA

Expression

Yeast

Promoter

GPD

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-GW

Plasmid

#63780

Purpose

yeast expression vector p414GPD modified such that it is Gateway converted and introduces a 3xFLAG tag at the N-terminus of the insert

Depositor

Susan Baserga

Article

McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

Type

Empty backbone

Tags

3xFLAG

Expression

Yeast

Promoter

GPD

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX2TK-Num1-PH

Plasmid

#71369

Purpose

bacterial expression of PH domain for phosphoinositide binding assay

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Num1-PH

Tags

GST

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa2563-2692

Promoter

tac

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
VPS35 shRNA1

Plasmid

#163858

Purpose

shRNA targeting the coding sequences of VPS35

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

VPS35 retromer complex component (VPS35 Human)

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBM19-sd4U

Plasmid

#179692

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE4-DddA-G1397-C (T1413I)

Expression

Bacterial

Mutation

T1413I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-SNAP-MD-SOX2

Plasmid

#115687

Purpose

Constitutive expression of a SOX2 protein tagged with SNAP-tag and a mitotic degron (MD)

Depositor

David Suter

Article

Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

Insert

SNAP-MD-SOX2

Use

Lentiviral

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRS426-GFP-Num1-PH

Plasmid

#71374

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Num1-PH

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa2563-2692

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pBM13h-sd4U

Plasmid

#179690

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE3-DddA-G1397-N wildtype

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMIR-R/Tac1/SG

Plasmid

#16296

Depositor

Pranela Rameshwar

Article

Greco et al Proc Natl Acad Sci U S A. 2007 Sep 25. 1

Insert

Tac1 3'UTR (TAC1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pUAST tdTomato mouse Rab35 DN

Plasmid

#53513

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Tags

tdtomato

Expression

Insect

Mutation

S22N

Promoter

GAL4

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pUAST YFP mouse Rab35 WT

Plasmid

#46014

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pUAST YFP mouse Rab35 DN

Plasmid

#46016

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S22N (dominant negative)

Promoter

GAL4

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pUAST myc mouse Rab35 CA

Plasmid

#53516

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Tags

myc

Expression

Insect

Mutation

Q67L

Promoter

GAL4

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
tdTomato-Cx43-7

Plasmid

#58086

Purpose

Localization: Gap Junctions, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Cx43

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pUAST myc mouse Rab35 DN

Plasmid

#53542

Purpose

expression of myc tagged mouse Rab35 DN mutant

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Tags

myc

Expression

Insect

Mutation

S22N

Promoter

GAL4

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pUAST tdTomato mouse Rab35 CA

Plasmid

#53512

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Tags

tdtomato

Expression

Insect

Mutation

Q67L

Promoter

GAL4

Available Since

May 12, 2014

Availability

Academic Institutions and Nonprofits only
pUAST YFP mouse Rab35 CA

Plasmid

#46015

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q67L (constitutively active)

Promoter

GAL4

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only