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pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

Plasmid

#159630

Purpose

AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

Depositor

Andrew Allen, Ross Bathgate

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

Ast (AstA Fly)

Use

AAV

Tags

HA, IRES, mCherry, and neurophysin-1

Expression

Mammalian

Promoter

DCA (same as CAG promoter)

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAc-mCh-Tub

Plasmid

#24288

Depositor

Ron Vale

Article

Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

Insert

Alpha-Tubulin (alphaTub84B Fly)

Tags

mCherry

Expression

Insect

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only
KHBD00370

Plasmid

#34485

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8409 (Su(var)205 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pCFD3-Act5c

Plasmid

#130278

Purpose

Drosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

pCFD3-Act5c (Act5C Fly)

Use

CRISPR

Expression

Insect

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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zw3 (shaggy) pbOVI

Plasmid

#38749

Depositor

Norbert Perrimon

Article

Siegfried et al Nature. 1990 Jun 28;345(6278):825-9.

Insert

zw3 (sgg Fly)

Expression

Bacterial

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pLX307/PTPN11 T73I

Plasmid

#140939

Purpose

expresses PTPN11 T73I

Depositor

Kimberly Stegmaier

Article

Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

Insert

PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)

Tags

V5

Expression

Mammalian

Mutation

T73I

Promoter

EF1a

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLX307/PTPN11 E76G

Plasmid

#140862

Purpose

expresses PTPN11 E76G

Depositor

Kimberly Stegmaier

Article

Cremer et al Cancer Discov. 2020 Feb;10(2):214-231. d

Insert

PTPN11 protein tyrosine phosphatase non-receptor type 11 [ Homo sapiens (human) ] (PTPN11 Human)

Expression

Mammalian

Mutation

E76G

Promoter

EF1a

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00334

Plasmid

#34457

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11491 (br Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pMXs-Puro-DUS2L-PSKH1

Plasmid

#69472

Purpose

Expresses DUS2L-PSKH1 fusion gene

Depositor

Axel Hillmer

Article

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

DUS2L-PSKH1 (PSKH1 Human)

Use

Retroviral

Tags

HA TAG

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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KHBD00534

Plasmid

#39632

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6103 (CrebB Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00612

Plasmid

#39688

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11648 (Abd-B Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00274

Plasmid

#34402

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1856 (ttk Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pUb FLAG-Mts D85N

Plasmid

#208403

Purpose

Expresses FLAG-tagged Drosophila mts protein with D85N mutation

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

microtubule star (mts Fly)

Tags

3xFLAG

Expression

Insect

Mutation

D85N

Promoter

Ubi-p63e

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Mts H59Q

Plasmid

#208402

Purpose

Expresses FLAG-tagged Drosophila mts protein with H59Q mutation

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

microtubule star (mts Fly)

Tags

3xFLAG

Expression

Insect

Mutation

H59Q

Promoter

Ubi-p63e

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-Mts H241A

Plasmid

#208404

Purpose

Expresses FLAG-tagged Drosophila mts protein with H241A mutation

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

microtubule star (mts Fly)

Tags

3xFLAG

Expression

Insect

Mutation

H241A

Promoter

Ubi-p63e

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pnCS_DSep2_Peanut-TEV-Strep

Plasmid

#174495

Purpose

bacterial co-expression of Drosophila Sep2 and of Drosophila Peanut

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

Sep2 (Sep2 Fly)
peanut (pnut Fly)

Tags

TEV-Strep

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry

Plasmid

#159631

Purpose

expresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoter

Depositor

Andrew Allen, Ross Bathgate

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

Ast (AstA Fly)

Use

Lentiviral

Tags

HA, IRES, mCherry, and neurophysin-1

Expression

Mammalian

Promoter

CMV promoter

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00450

Plasmid

#39563

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6103 (CrebB Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00026

Plasmid

#34188

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8333 (E(spl)mgamma-HLH Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00053

Plasmid

#34215

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6103 (CrebB Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only