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p5064 pcDNA3.1/V5-His-TOPO hBrd4 (1-461)

Plasmid

#14443

Depositor

Peter Howley

Article

You et al Cell. 2004 Apr 30. 117(3):349-60.

Insert

Brd4 (1-461) (BRD4 Human)

Expression

Mammalian

Mutation

amino acids 1-461

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pHL417

Plasmid

#62822

Purpose

Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::micC::Asp terminator + pLtetO-1::RBS (st7) hfq::T1 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

gfp
micC leader
hfq leader

Use

Synthetic Biology

Tags

ompC leader and st7 RBS

Expression

Bacterial

Promoter

pLlacO-1 and pLtetO-1

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pYTRW10K_0x5

Plasmid

#177278

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

Plasmid

#223163

Purpose

2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Type

Empty backbone

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pJM166B

Plasmid

#218123

Purpose

For hybrid circuit activation. Expresses E. coli RNAP omega subunit used to FRB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

FRB (MTOR Human)

Tags

rpoZ

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF-C-del331-370-YYMYFWFtoAAAAAAA_AH

Plasmid

#148910

Purpose

Insect Expression of DmGIGYF-del331-370-YYMFWFtoAAAAAA

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF-del331-370-YYMFWFtoAAAAAA (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmGIGYF-del331-370-YFWFtoAAAA_AH

Plasmid

#148912

Purpose

Insect Expression of DmGIGYF-del331-370-YFWFtoAAAA

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF-del331-370-YFWFtoAAAA (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmGIGYF-del331-370-YFWFtoAAAA-dsRNAres_AH

Plasmid

#148927

Purpose

Insect Expression of DmGIGYF1-del331-370-YFWFtoAAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF1-del331-370-YFWFtoAAAA-dsRNAres (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvM-DmBam_1-140_AG

Plasmid

#148832

Purpose

Bacterial Expression of DmBam_1-140

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_1-140 (bam Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHelz_AH

Plasmid

#148881

Purpose

Insect Expression of DmHelz

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

DmHelz (CG9425 Fly)

Expression

Insect

Mutation

two silent an two non silent G1374S, R2076G mutat…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHelz_AH

Plasmid

#148885

Purpose

Insect Expression of DmHelz

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

DmHelz (CG9425 Fly)

Expression

Insect

Mutation

two silent an two non silent G1374S, R2076G mutat…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHelz_1213-2103_AH

Plasmid

#148887

Purpose

Insect Expression of DmHelz_1213-2103

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

DmHelz_1213-2103 (CG9425 Fly)

Expression

Insect

Mutation

two silent an two non silent G1374S, R2076G mutat…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHelz-E958Q_AH

Plasmid

#148888

Purpose

Insect Expression of DmHelzE958Q

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

DmHelzE958Q (CG9425 Fly)

Expression

Insect

Mutation

two silent an two non silent G1374S, R2076G mutat…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBam_1-140_AG

Plasmid

#148815

Purpose

Insect Expression of DmBam_1-140

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_1-140 (bam Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBam_1-140_AG

Plasmid

#148820

Purpose

Bacterial Expression of DmBam_1-140

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_1-140 (bam Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-GGSG-DmBam_13-36_AG

Plasmid

#148827

Purpose

Insect Expression of DmBam_13-36

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_13-36 (bam Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-DmCaf40_25-291-V186E_AF

Plasmid

#148735

Purpose

Bacterial Expression of DmCaf40_25-291-V186E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmCaf40_25-291-V186E (Rcd-1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot6_AF

Plasmid

#148738

Purpose

Bacterial Expression of HsNot6

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot6 (CNOT6 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmNot1_1468-1719_AF

Plasmid

#148740

Purpose

Bacterial Expression of DmNot1_1468-1719

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmNot1_1468-1719 (Not1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-DmRoquin_702-819-del790-812-GB1His6_AE

Plasmid

#148623

Purpose

Bacterial Expression of DmRoquin_702-819-del790-812

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

DmRoquin_702-819-del790-812 (roq Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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