Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 13,664 results for: 109

Showing: 4001 - 4020 of 13664 results

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pET-PLS-KP6-C93A-C96A_rpoA-C

Plasmid

#190970

Purpose

Test the C-to-U editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:C93A/C96A

Promoter

T7

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP6-A66S_rpoA-C

Plasmid

#190988

Purpose

Test the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Mammalian

Mutation

DYW-KP:A66S

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP6-C93A-C96A_rpoA-U

Plasmid

#190991

Purpose

Test the U-to-C editing activity of KP6:C93A/C96A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Mammalian

Mutation

DYW-KP:C93A/C96A

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP2-C93A-C96A_rpoA-U

Plasmid

#190958

Purpose

Test the U-to-C editing activity of KP2:C93A/C96A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Bacterial

Mutation

DYW-KP:C93A/C96A

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PM18033-PLS-KP2-E67A_rpoA-U

Plasmid

#190979

Purpose

Test the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Mammalian

Mutation

DYW-KP:E67A

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP3-E67A_rpoA-U

Plasmid

#190983

Purpose

Test the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Mammalian

Mutation

DYW-KP:E67A

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP3-C93A-C96A_rpoA-U

Plasmid

#190982

Purpose

Test the U-to-C editing activity of KP3:C93A/C96A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Mammalian

Mutation

DYW-KP:C93A/C96A

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PLS-KP6-C93A-C96A_rpoA-U

Plasmid

#190971

Purpose

Test the U-to-C editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:C93A/C96A

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP6-C93A-C96A_rpoA-C

Plasmid

#190990

Purpose

Test the C-to-U editing activity of KP6:C93A/C96A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Mammalian

Mutation

DYW-KP:C93A/C96A

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP2-C93A-C96A_rpoA-U

Plasmid

#190978

Purpose

Test the U-to-C editing activity of KP2:C93A/C96A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Mammalian

Mutation

DYW-KP:C93A/C96A

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP3-A66S_rpoA-U

Plasmid

#190981

Purpose

Test the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Mammalian

Mutation

DYW-KP:A66S

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
PM18033-PLS-KP6-E67A_rpoA-U

Plasmid

#190993

Purpose

Test the U-to-C editing activity of KP6:E67A on AtrpoA editing site, in human cells

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Mammalian

Mutation

DYW-KP:E67A

Promoter

CMV

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
QUAS-0-T7-TetO-GFP_T7-LacO-TetR_ColE1

Plasmid

#171663

Purpose

QUAS directly upstream of a T7 promoter with a TetO operator. Expression of TetR in BL21(DE3) E. coli. GFP is expressed according to presence of aTc. Contains the ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

QUAS-T7-TetO-GFP ssrA-T7 Stop- T7-LacO-TetR-T7 Stop

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAcrVIB1-cloDF-kan

Plasmid

#184836

Purpose

Expression of anti-CRISPR protein AcrVIB1 in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

anti-CRISPR protein inhibiting Cas13b nucleases

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-doraWT_rescue_construct

Plasmid

#190608

Purpose

Puromycin-selectable expression of HA-tagged Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Tags

3xHA

Expression

Insect

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc-sgRNA-Cas9.dora_1

Plasmid

#190700

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 1

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc-sgRNA-Cas9.dora_2

Plasmid

#190701

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 2

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc-sgRNA-Cas9.dora_3

Plasmid

#190702

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 3

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
iLID-mRuby3-iTrkA

Plasmid

#136510

Purpose

Mammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkA (aa 450-799)

Depositor

Bianxiao Cui

Article

Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

Insert

iLID mRuby3 iTrkA (Ntrk1 Rat, Avena sativa)

Expression

Mammalian

Promoter

CMV

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCASCADE-xylA

Plasmid

#158611

Purpose

Low phosphate inducible gRNA silencing the xylA promoter

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

xylA guide array

Expression

Bacterial

Promoter

E . coli ugpB gene promoter, low phosphate induct…

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 4001 - 4020 of 13664 results

Results per page:

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