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Showing: 10051 - 10100 of 79968 results

  1. pFastBac HTB His-TEV-rDPP9-FLAG

    Plasmid
    #217328
    Purpose
    Purification of rat DPP9 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    DPP9
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217328-plasmid-map-sequence-id-433437

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  2. pFastBac HTB His-TEV-rDPP9(S729A)

    Plasmid
    #217327
    Purpose
    Purification of catalytically-dead rat DPP9 from insect cells
    Depositor
    Yifan Wang
    Insert
    DPP9
    Tags
    His-TEV
    Expression
    Insect
    Mutation
    S729A
    Promoter
    polyhedrin
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217327-plasmid-map-sequence-id-433438

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  3. pET29a-DDX53-Q249A

    Plasmid
    #222216
    Purpose
    Express DDX53 mutant Q249A protein in bacteria
    Depositor
    Yuliang Wu
    Insert
    DDX53_Q249A (DDX53 Human)
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    K272A
    Promoter
    T7
    Available Since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222216-plasmid-map-sequence-id-441427

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  4. pET29a-DDX53-K272A

    Plasmid
    #222215
    Purpose
    Express DDX53 mutant K272A protein in bacteria
    Depositor
    Yuliang Wu
    Insert
    DDX53_K272A (DDX53 Human)
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    K272A
    Promoter
    T7
    Available Since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222215-plasmid-map-sequence-id-441662

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  5. pET29a-DDX53-E377Q

    Plasmid
    #222217
    Purpose
    Express DDX53 mutant E377Q protein in bacteria
    Depositor
    Yuliang Wu
    Insert
    DDX53_E377Q (DDX53 Human)
    Tags
    His Tag
    Expression
    Bacterial
    Mutation
    E377Q
    Promoter
    T7
    Available Since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    222217-plasmid-map-sequence-id-441653

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  6. LT3GEPIR-NEK8shRNA1

    Plasmid
    #220552
    Purpose
    To inducibly knockdown NEK8 expression
    Depositor
    Sean Lee
    Insert
    NEK8 shRNA1 (NEK8 Human)
    Use
    Lentiviral
    Promoter
    TRE3G (TetOP) promoter
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220552-plasmid-map-sequence-id-437958

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  7. LT3GEPIR-NEK8shRNA2

    Plasmid
    #220553
    Purpose
    To inducibly knockdown NEK8 expression
    Depositor
    Sean Lee
    Insert
    NEK8 shRNA2 (NEK8 Human)
    Use
    Lentiviral
    Promoter
    TRE3G (TetOP) promoter
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220553-plasmid-map-sequence-id-437955

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  8. LT3GEPIR-NEK9shRNA1

    Plasmid
    #220554
    Purpose
    To inducibly knockdown NEK9 expression
    Depositor
    Sean Lee
    Insert
    NEK9 shRNA1 (NEK9 Human)
    Use
    Lentiviral
    Promoter
    TRE3G (TetOP) promoter
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220554-plasmid-map-sequence-id-437953

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  9. pOCC102-MBP-eIF4G-SkA

    Plasmid
    #219977
    Purpose
    eIF4G-SkA protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-SkA (TIF4631 Budding Yeast)
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    eIF4A binding domain of S. kudriavzevii
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219977-plasmid-map-sequence-id-436800

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  10. pOCC102-MBP-eIF4G-RAR

    Plasmid
    #219973
    Purpose
    eIF4G-RAR protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G-RAR (TIF4631 Budding Yeast)
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    Truncation: amino acids 490 to 942
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219973-plasmid-map-sequence-id-436750

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  11. His-GST-MPK6

    Plasmid
    #215170
    Purpose
    Encodes the glutathionine S-transferase with N-terminal His tag and fused to the N-terminus of a mitogen-activated protein kinase 6 from Arabidopsis thaliana
    Depositor
    Travis Beddoe, Bhuvana Shanbhag
    Insert
    His-GST-MPK6
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    T7 lac
    Available Since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    215170-plasmid-map-sequence-id-430166

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  12. mPLD3-sgRNA-Cas9-mcherry

    Plasmid
    #199700
    Purpose
    encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199700-plasmid-map-sequence-id-390617

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  13. hPLD4-sgRNA-Cas9_mcherry

    Plasmid
    #199343
    Purpose
    encodes sgRNA for human PLD4 KO, (target Exon 5) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-mCherry
    Expression
    Mammalian
    Mutation
    sgRNA: accagtagtatgaagccacg
    Promoter
    human U6
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    199343-plasmid-map-sequence-id-390620

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  14. pLSU1/t35s[TMV]:MCS+:NosT

    Plasmid
    #212160
    Purpose
    This binary vector has an empty backbone with extended multiple cloning site (MSC) where you can inserta a gene into a cassette with a strong promoter (truncated 35sCAMV) and UTR (Tomato Mosaic Virus)
    Depositor
    Wayne Curtis
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212160-plasmid-map-sequence-id-422551

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  15. pLSU1/t35S[TMV]:mNeonGreen:NosT

    Plasmid
    #212162
    Purpose
    This binary vector expresses mNeonGreen with a truncated 35sCAMV promoter with strong UTR (Tomato Mosaic Virus)
    Depositor
    Wayne Curtis
    Insert
    Tomato codon optimized version of mNeonGreen
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato
    Promoter
    truncated 35sCaMV
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212162-plasmid-map-sequence-id-422667

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  16. PTNA-c200

    Plasmid
    #205114
    Purpose
    PTN protein expression
    Depositor
    Paul Brennan
    Insert
    PTN (PTN Human)
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205114-plasmid-map-sequence-id-411200

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  17. pCAGGS-iRFP713(C15S/V256C)-P2A-mCherry

    Plasmid
    #198069
    Purpose
    Mammalian expression of iRFP713(C15S/V256C) and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    iRFP713(C15S/V256C)
    Tags
    P2A-mCherry
    Expression
    Mammalian
    Mutation
    C15S/V256C mutation
    Promoter
    CAG
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198069-plasmid-map-sequence-id-389877

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  18. pCSIIbleo-miRFP670nano(R57C/C86S)-P2A-EGFP

    Plasmid
    #197370
    Purpose
    The lentiviral vector for miRFP670nano(R57C/C86S) and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    miRFP670nano(R57C/C86S)
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    R57C/C86S mutation
    Promoter
    CAG
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197370-plasmid-map-sequence-id-389688

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  19. pCSIIbleo-6504-P2A-EGFP

    Plasmid
    #197368
    Purpose
    The lentiviral vector for NIR-GECOHI and EGFP
    Depositor
    Michiyuki Matsuda
    Insert
    NIR-GECO2(C18S/I426C)
    Use
    Lentiviral
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    C18S/I426C mutation
    Promoter
    CAG
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197368-plasmid-map-sequence-id-389693

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  23. pQE80l B-CC-B

    Plasmid
    #196479
    Purpose
    Protein expression of SpyCatcher-ELP-CarHCC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCC
    Depositor
    Fei Sun
    Insert
    B-CC-B
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    196479-plasmid-map-sequence-id-387244

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  24. sfCherry11-Ex1-17-PTPN22

    Plasmid
    #195387
    Purpose
    Mammalian expression of a human PTPN22 mutant (exon 1-17) with sfCherry(11) tag
    Depositor
    Patrick Concannon
    Insert
    PTPN22 (PTPN22 Human)
    Tags
    sfCherry(11)
    Expression
    Mammalian
    Mutation
    PTPN22 mutant containing exon 1 to 17
    Available Since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195387-plasmid-map-sequence-id-387974

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  26. pGEX-6P-1-PDE-short-UBASH3A

    Plasmid
    #195403
    Purpose
    Bacterial expression of a putative PDE domain of human short-form UBASH3A with GST tag
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    PDE domain of UBASH3A
    Available Since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    195403-plasmid-map-sequence-id-386313

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  27. ipUSEPR-sg-Hs-CD4

    Plasmid
    #188691
    Purpose
    sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgCD4 (CD4 Human)
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available Since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188691-plasmid-map-sequence-id-375118

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  28. ipUSEPR-sg-Hs-PHGDH-int2

    Plasmid
    #188682
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPHGDH intron control (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available Since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188682-plasmid-map-sequence-id-375035

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  29. ipUSEPR-sg-Hs-PHGDH-int1

    Plasmid
    #188681
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPHGDH intron control (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available Since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188681-plasmid-map-sequence-id-375018

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  30. ipUSEPR-sg-Hs-PHGDH-int3

    Plasmid
    #188683
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPHGDH intron control (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available Since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188683-plasmid-map-sequence-id-375016

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  31. pSB4A3-PBAD-phzSM

    Plasmid
    #165620
    Purpose
    Arabinose-inducible expression of phzSM for conversion of phenazine-1-carboxylic acid to pyocyanin
    Depositor
    Martin Buck
    Insert
    phzSM (phzS, phzM Pseudomonas aeruginosa)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    Oct. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    165620-plasmid-map-sequence-id-331407

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  33. pENN.AAV.SV40-si-CS

    Plasmid
    #187861
    Purpose
    a human siRNA-expressing plasmid targeting CS
    Depositor
    Yan Zeng
    Insert
    si-CS (CS Human)
    Use
    AAV
    Available Since
    Aug. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    187861-plasmid-map-sequence-id-370178

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  34. pAAV.CMV.PI-si-CS

    Plasmid
    #187857
    Purpose
    a human siRNA-expressing plasmid targeting CS
    Depositor
    Yan Zeng
    Insert
    si-CS (CS Human)
    Use
    AAV
    Available Since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    187857-plasmid-map-sequence-id-370021

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  35. pSuper-si-CS

    Plasmid
    #187854
    Purpose
    a human siRNA-expressing plasmid targeting CS
    Depositor
    Yan Zeng
    Insert
    si-CS (CS Human)
    Expression
    Mammalian
    Available Since
    Aug. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    187854-plasmid-map-sequence-id-369881

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  36. KBB258

    Plasmid
    #185072
    Purpose
    Bacterial expression of N-terminus and FXFG repeats of NUP1 nucleoporin as GST-Nup1-N+Rep fusion
    Depositor
    Ken Belanger
    Insert
    NUP1
    Tags
    GST
    Expression
    Yeast
    Mutation
    Nup1 N-term+FXFG repeats
    Available Since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185072-plasmid-map-sequence-id-368132

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  37. pGAE-UFO.664

    Plasmid
    #183471
    Purpose
    Lentiviral vector expressing HIV Envelope UFO.664
    Depositor
    Andrea Cara
    Insert
    HIV envelope
    Use
    Lentiviral
    Promoter
    CMV
    Available Since
    April 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    183471-plasmid-map-sequence-id-360575

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  38. pGAE-UFO.750

    Plasmid
    #183472
    Purpose
    Lentiviral vector expressing HIV Envelope UFO.750
    Depositor
    Andrea Cara
    Insert
    HIV Envelope
    Use
    Lentiviral
    Promoter
    CMV
    Available Since
    April 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    183472-plasmid-map-sequence-id-360574

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  39. pLNKR2

    Plasmid
    #67969
    Purpose
    High copy number plasmid encoding part of the tra Operon traC-artA
    Depositor
    Joshua Leonard
    Insert
    tra Operon (second Quarter)
    Use
    Synthetic Biology
    Available Since
    Feb. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    67969-plasmid-map-sequence-id-121291

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  40. pPD719 DsRed-Express2-ZF173

    Plasmid
    #138811
    Purpose
    Constitutive expression of DsRed-Express2-ZF173 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF173
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    138811-plasmid-map-sequence-id-322832

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  41. pUPD2 NtSPrbcS (GB2213)

    Plasmid
    #160589
    Purpose
    B3 for signal peptide (transit peptide to chloroplast) from Ribulose bisphosphate carboxylase small chain
    Depositor
    Diego Orzaez
    Insert
    NtSPrbcS
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160589-plasmid-map-sequence-id-322077

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  42. AtS/MAR10 insulator sequence (GB3459)

    Plasmid
    #160639
    Purpose
    pEGB3a2 AtS/MAR10
    Depositor
    Diego Orzaez
    Insert
    AtS/MAR10 insulator sequence
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160639-plasmid-map-sequence-id-321955

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  43. pPD718 DsRed-Express2-ZF172

    Plasmid
    #138810
    Purpose
    Constitutive expression of DsRed-Express2-ZF172 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF172
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    138810-plasmid-map-sequence-id-320933

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  44. pPD717 DsRed-Express2-ZF151

    Plasmid
    #138808
    Purpose
    Constitutive expression of DsRed-Express2-ZF151 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF151
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    138808-plasmid-map-sequence-id-320928

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  45. pPD707 DsRed-Express2-ZF14

    Plasmid
    #138794
    Purpose
    Constitutive expression of DsRed-Express2-ZF14 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF14
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138794-plasmid-map-sequence-id-320377

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  46. pPD709 DsRed-Express2-ZF36

    Plasmid
    #138796
    Purpose
    Constitutive expression of DsRed-Express2-ZF36 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF36
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138796-plasmid-map-sequence-id-320735

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  47. pPD710 DsRed-Express2-ZF42

    Plasmid
    #138798
    Purpose
    Constitutive expression of DsRed-Express2-ZF42 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF42
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138798-plasmid-map-sequence-id-320741

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  48. pPD711 DsRed-Express2-ZF54

    Plasmid
    #138800
    Purpose
    Constitutive expression of DsRed-Express2-ZF54 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF54
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138800-plasmid-map-sequence-id-320744

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  49. pPD712 DsRed-Express2-ZF55

    Plasmid
    #138801
    Purpose
    Constitutive expression of DsRed-Express2-ZF55 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF55
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138801-plasmid-map-sequence-id-320747

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  50. pPD713 DsRed-Express2-ZF62

    Plasmid
    #138802
    Purpose
    Constitutive expression of DsRed-Express2-ZF62 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF62
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    138802-plasmid-map-sequence-id-320750

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Showing: 10051 - 10100 of 79968 results