We narrowed to 4,353 results for: erf

1. pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB

   Plasmid

   #154473

   Purpose

   Expresses dCas9 fused to mCherry and the KRAB domain of ZIM3

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ZIM3 (ZIM3 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Includes ZIM3 aa 1-100

   Promoter

   SFFV

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MOM603

   Plasmid

   #133242

   Purpose

   expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

   Depositor

   Shinsuke Niwa

   Article

   Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

   Insert

   kif1a (KIF1A Human)
   

   Use

   Tags

   StrepII tag and mScarlet

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9-GFP

   Plasmid

   #181906

   Purpose

   Fluorescently tagged dead Cas9

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

   Plasmid

   #188778

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-UCOE-SFFV-Zim3-dCas9-P2A-BFP

   Plasmid

   #188767

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-dCas9-KRAB-PGK-HygR

   Plasmid

   #83890

   Purpose

   Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   humanized dead Cas9 KRAB
   aminoglycoside phosphotransferase from E. coli
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Human Ubiquitin C Promoter and mouse phosphoglyce…

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry

   Plasmid

   #188779

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A

   Plasmid

   #207953

   Purpose

   nuclease inactive control for TRF1FokI WT

   Depositor

   Roger Greenberg

   Article

   Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

   Insert

   TRF1 (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag, DD, ER, mCherry and FokI (D450A)

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEND-351_Cacnes_dCas9_CRISPRi

   Plasmid

   #225617

   Purpose

   Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Promoter

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTMB301_ZFcharm_Kv1_Prnp_SPM

    Plasmid

    #220840

    Purpose

    AAV genome expressing ZFcharm Kv1 targeting Prnp; single perfect match self-silencing binding site

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm Kv1
    

    Use

    AAV

    Tags

    HAtag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. plenti-CAG-IRF4-FLAG-IRES-GFP

    Plasmid

    #107389

    Purpose

    Lentiviral expression of IRF4-FLAG

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IRF4 (IRF4 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIA33

    Plasmid

    #120803

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp

    Depositor

    David S. Weiss, Craig Ellermeier

    Article

    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

    Inserts

    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Base-pairing region targets red fluorescent prote…

    Promoter

    Pgdh and Pxyl

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMH0001

    Plasmid

    #85969

    Purpose

    UCOE-SFFV-dCas9-BFP-KRAB

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    BFP-KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    SFFV

    Available Since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-dL5**-mCER-TRF1

    Plasmid

    #168176

    Purpose

    Expresses FAP (dL5**) fused to mCerulean and the telomere binding protein TRF1

    Depositor

    Patricia Opresko

    Article

    Fouquerel et al Mol Cell. 2019 Jul 11;75(1):117-130.e6.

    Insert

    TRF1 (TERF1 Human)
    

    Use

    Lentiviral

    Tags

    FAP (dL5**) and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-UCOE-EF1a-Zim3-dCas9-BFP

    Plasmid

    #188775

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9-mTagBFP2
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-UbC

    Plasmid

    #62806

    Purpose

    To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

    Depositor

    Pantelis Tsoulfas, Jae Lee

    Article

    Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

    Type

    Empty backbone

    Use

    AAV; Eucaryotic

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-UCOE-SFFV-Zim3-dCas9-BFP

    Plasmid

    #188776

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9-mTagBFP2
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLL7 lenti-dcas9-3xflag-blast

    Plasmid

    #112133

    Purpose

    lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    dCas9(D10A, N863A)-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A mutants in Cas9

    Promoter

    EF1a

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMH0006

    Plasmid

    #135448

    Purpose

    Human expression vector containing Ubiquitous Chromatin Opening Element (UCOE) upstream of EF1alpha promoter, dCas9 that is fused to NLS, tagBFP and a KRAB domain.

    Depositor

    Jonathan Weissman, Martin Kampmann

    Article

    Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

    Insert

    dCas9-BFP-KRAB
    

    Use

    Lentiviral

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    July 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. plenti-UBC- IRF4-3xHA-pGK-PUR

    Plasmid

    #107386

    Purpose

    Lentiviral expression of IRF4-3xHA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IRF4 (IRF4 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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