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We narrowed to 9,372 results for: bli

Showing: 8081 - 8120 of 9372 results
  1. NanoBRET Assay Vector, NanoLuc-NRAS(Q61R)

    Plasmid
    #236863
    Purpose
    Express NanoLuc(R)-NRAS Q61R in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Insert
    NRAS (NRAS Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    Q61R
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  2. NanoBRET Assay Vector, NanoLuc-NRAS(Q61K)

    Plasmid
    #236854
    Purpose
    Express NanoLuc(R)-NRAS(Q61K) in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Insert
    NRAS (NRAS Human)
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    Q61K
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. NanoBRET Assay Vector, HaloTag-KRAS(G13D)

    Plasmid
    #236856
    Purpose
    Express HaloTag(R)-KRAS(G13D) in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    G13D
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. SmBiT-KRAS4B(G12D) BiBiT-Ready Vector

    Plasmid
    #236798
    Purpose
    Express SmBiT-KRAS4B(G12D) Fusion Protein in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Insert
    KRAS4B(G12D) (KRAS Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    G12D
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid
    #239448
    Purpose
    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.
    Depositor
    Inserts
    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    BFP: T65S, S72S, V145F, K206K, H231L
    Promoter
    EF1alpha, U6, and mU6
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. rTetR-GFP-p65

    Plasmid
    #232633
    Purpose
    p65 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition
    Depositor
    Insert
    rTetR-GFP-p65 (RELA Human, Synthetic)
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pdCas9-GFP-p65

    Plasmid
    #232630
    Purpose
    p65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacer
    Depositor
    Insert
    dCas9-GFP-p65 (RELA Human, Synthetic)
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. Kif3A-EGFP-SNAP

    Plasmid
    #229852
    Purpose
    Kif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experiments
    Depositor
    Insert
    Kif3A (Kif3a Mouse)
    Tags
    6xHis, EGFP, and SNAP-tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pLANT-2b

    Plasmid
    #231487
    Purpose
    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.
    Depositor
    Inserts
    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    Tags
    His6 tag and His6-SUMO^NIT
    Expression
    Bacterial
    Mutation
    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…
    Promoter
    T7 promoter
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. E547* FL USP7

    Plasmid
    #229001
    Purpose
    E547-mutant of human USP7
    Depositor
    Insert
    USP7 1-546 (USP7 Human)
    Tags
    6xHis cleavable with thrombin
    Expression
    Bacterial
    Mutation
    E547 mutated to stop codon
    Promoter
    T7
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pCAGGS-Twin-strep-TEV-DGKδ2

    Plasmid
    #223720
    Purpose
    Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Insert
    DGKD (DGKD Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted start codon
    Promoter
    CAG and chicken β-actin promoter
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. pCAGGS-PHOSPHO1-TEV-Twin-Strep

    Plasmid
    #222015
    Purpose
    Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Insert
    PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    stop codon (TGA) is deleted
    Promoter
    chicken β-actin promoter
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. pDGB3_alpha2_BeYDV geminino 2.0 (no ATG)-eGFP (GB5178)

    Plasmid
    #225443
    Purpose
    Geminino 2.0-no ATG on CDS. BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1.
    Depositor
    Insert
    BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

    Plasmid
    #219823
    Purpose
    EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)
    Depositor
    Insert
    RfxCas13d
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a core promoter
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
  15. pWR23

    Plasmid
    #202770
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coKanMX
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Expression
    Yeast
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  16. pWR10

    Plasmid
    #202710
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coShBle
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Expression
    Yeast
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  17. pWR9

    Plasmid
    #202645
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Expression
    Yeast
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  18. SUMO CRYGS 16-177 Q16C

    Plasmid
    #217673
    Purpose
    N-terminally truncated human gamma S crystallin residues 16-177 with Q16C mutation for native chemical ligation
    Depositor
    Insert
    CRYGS (CRYGS Human)
    Tags
    6XHis and SUMO
    Expression
    Bacterial
    Mutation
    Glutamine 16 changed to Cysteine, and amino acids…
    Promoter
    T7
    Available Since
    May 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  19. pMamCol1

    Plasmid
    #215184
    Purpose
    Allows for stable expression of recombinant (tagged/untagged) proteins in AAVS1 locus of Human cells and transient Protein Expression in E. coli
    Insert
    tsPurple
    Use
    AAV, CRISPR, and Synthetic Biology ; Recombinant …
    Tags
    SNAC-StrepII tag
    Expression
    Bacterial and Mammalian
    Promoter
    TetON 3G Bidirectional for Mammalian and Trc with…
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  20. pUC-T7-hTP53-P0~670-FLuc

    Plasmid
    #208640
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.
    Depositor
    Insert
    TP53, UTR and partial CDS (TP53 Human)
    Use
    Luciferase; In vitor transcription template
    Tags
    Firefly Luciferase (F-Luc) and T7 promoter
    Mutation
    UTR and partial CDS in-frame with F-Luc
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  21. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

    Plasmid
    #208645
    Purpose
    Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.
    Depositor
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
    Use
    CRISPR; Ssdna donnor preparation for knock-in of…
    Tags
    Twin Strep
    Mutation
    Two SNP (rs749555789, rs760194863) were introduce…
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  22. pWR54

    Plasmid
    #203330
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Expression
    Yeast
    Available Since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
  23. pWR24

    Plasmid
    #202771
    Purpose
    episomal bioluminescent reporter plasmid for S. stipitis
    Depositor
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coNAT
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    Expression
    Yeast
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
  24. pWR16

    Plasmid
    #202711
    Purpose
    integrating bioluminescent reporter plasmid for S. stipitis
    Depositor
    Inserts
    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    URA3 and flanking sequence
    Expression
    Yeast
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
  25. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

    Plasmid
    #200389
    Purpose
    3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression
    Insert
    PX458_3XHA_dFnCas9_KRAB_eGFP
    Use
    CRISPR
    Tags
    3xHA-NLS and SV40NLS-KRAB-T2A-EGFP
    Expression
    Mammalian
    Promoter
    CBH
    Available Since
    Sept. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
  26. pLV-SFFV-Llgl1-WPRE-UbC-Emerald

    Plasmid
    #203805
    Purpose
    Lentiviral vector plasmid expressing mouse Llgl1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Inserts
    Llgl1 (Llgl1 Mouse)
    Emerald
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    July 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  27. pGEX-6p-1_GST-DmKHC[1-421]-1xGFP

    Plasmid
    #196973
    Purpose
    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and cleavable N-terminal GST tag
    Depositor
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Tags
    GFP and GST
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  28. pGEX-6p-1_GST-SNAP-DmKHC[1-421]

    Plasmid
    #196976
    Purpose
    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal SNAP tag and cleavable N-terminal GST
    Depositor
    Insert
    Kinesin Heavy Chain (Khc Fly)
    Tags
    GST and SNAP
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    July 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  29. pT5T_Rev7_1-211_SA112_KVK_Rev3_1873-1898

    Plasmid
    #105635
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1873-1898) from bicistronic plasmid
    Depositor
    Inserts
    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  30. pT5T_Rev7_1-211_SA112_KVK_Rev3_1989-2014

    Plasmid
    #105637
    Purpose
    Expresses mouse monomeric Rev7 and fragment of Rev3 protein (amino acids 1989-2014) from bicistronic plasmid
    Depositor
    Inserts
    Rev7 (Mad2l2 Mouse)
    Rev3l (REV3L Human)
    Expression
    Bacterial
    Mutation
    Mutations: F11S, G12A, V132K, C133V, A135K
    Promoter
    T7
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 8081 - 8120 of 9372 results