We narrowed to 26,528 results for: CAL;

1. M23: pHR-SFFVp-FUSN::mCherry::SspB

   Plasmid

   #122148

   Purpose

   N terminal domain of FUS (1-214) tagged with SspB and mCherry.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FUS (FUS Human)
   

   Use

   Lentiviral

   Tags

   mCherry-sspB

   Expression

   Mammalian

   Mutation

   Contains only amino acids 1-214

   Promoter

   SFFV

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB

   Plasmid

   #122668

   Purpose

   C terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   HNRNPA1 (HNRNPA1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-SspB

   Expression

   Mammalian

   Mutation

   Contains only amino acids 186-230

   Promoter

   SFFV

   Available since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGP-CMV-jGCaMP7s

   Plasmid

   #104463

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7s
   

   Use

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGP-CMV-jGCaMP7b

   Plasmid

   #104484

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7b
   

   Use

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-EF1a-FLEx-ASAP4e-Kv-WPRE

   Plasmid

   #201033

   Purpose

   Expresses somatically enriched ASAP4e from the EF-1alpha promoter in a cre-dependent manner

   Depositor

   Michael Lin

   Article

   Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

   Insert

   ASAP4b-Kv
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-EF1a-FLEx-ASAP4b-Kv-WPRE

   Plasmid

   #201032

   Purpose

   Expresses somatically enriched ASAP4b from the EF-1alpha promoter in a cre-dependent manner

   Depositor

   Michael Lin

   Article

   Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

   Insert

   ASAP4e-Kv
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. CRY2high-mCherry-Raf1

   Plasmid

   #104064

   Purpose

   Expresses fusion of CRY2high mutant (CRY2PHR E490R) with mCherry and Raf1

   Depositor

   Bianxiao Cui

   Article

   Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

   Insert

   CRY2high-mCherry-Raf1 (CRY2, RAF1 Human, Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   CRY2PHR E490R

   Promoter

   CMV

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-SspB-HaloTag-ITSN1-DHPH

   Plasmid

   #176128

   Purpose

   Heterodimerization with iLID, Cdc42 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   ITSN1 (ITSN1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-CIB1-mCerulean-MP

   Plasmid

   #58366

   Purpose

   For use in light-induced protein inactivation through the interaction with CRY2

   Depositor

   Won Do Heo

   Article

   Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

   Inserts

   CIB1[1-147] (CIB1 Mustard Weed)
   Camk2a[315-478] (Camk2a Rat)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNGVL4a-hCRTmE6mE7mL2

    Plasmid

    #104153

    Purpose

    Expresses human calreticulin fused to MmuPV1 E6, MmuPV1 E7 and MmuPV1 L2 residues 11-210 in mammalian cells

    Depositor

    Richard Roden

    Article

    Jiang et al J Virol. 2017 Jul 12;91(15). pii: e00699

    Insert

    hCRTmE6mE7mL2 11-210 (CALR, E6, E7, L2 Human, MmuPV1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    residues 11 to 200 of the late protein L2

    Promoter

    CMV

    Available since

    Dec. 18, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. p28-2iDMD-puro

    Plasmid

    #88895

    Purpose

    28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.

    Depositor

    Michele Calos

    Article

    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

    Inserts

    Dystrophin (DMD Human)
    luciferase
    puromycin resistance enzyme
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAcBac1-Mb-sfGFP 150TAG

    Plasmid

    #197570

    Purpose

    Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP 150TAG
    Pyl-tRNA (4 copies)
    

    Use

    Tags

    V5-His6

    Expression

    Mammalian

    Mutation

    N150TAG

    Promoter

    CMV and U6/H1

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. M31: pHR-SFFVp- TDP-43C::mCherry::SspB

    Plasmid

    #122669

    Purpose

    C terminal domain of TDP43 (218-414) tagged with SspB and mCherry.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    TDP43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry-SspB

    Expression

    Mammalian

    Mutation

    Contains only amino acids 218-414

    Promoter

    SFFV

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-BACE1-mBFP

    Plasmid

    #196697

    Purpose

    Expresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Beta-secretase 1 (BACE1 Human)
    

    Use

    Tags

    HA and mBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDule-3-nitroTyrosine (A7)

    Plasmid

    #174078

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32H H70T D158H I159A L162R

    Promoter

    GlnRS (constitutive)

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSAD-5PSD95-EGFP-SynPhRFP

    Plasmid

    #217979

    Purpose

    G-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFP

    Depositor

    Edward Callaway

    Article

    Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

    Inserts

    5PSD95-EGFP (Dlg4 Rat)
    SynPhRFP (Syp Mouse)
    

    Use

    G-deleted rabies

    Tags

    EGFP and RFP

    Expression

    Mutation

    Promoter

    None

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICE-HA-Ku70-siR-Mut6E

    Plasmid

    #82330

    Purpose

    Plasmid for constitutive or doxy-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10

    Insert

    Ku70 (XRCC6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Mut6E corresponding to K282E K287E T300E K331E K3…

    Promoter

    CMV-tet

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-CIB1-mRFP1-MP

    Plasmid

    #58367

    Purpose

    For use in light-induced protein inactivation through the interaction with CRY2

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Inserts

    CIB1[1-147] (CIB1 Mustard Weed)
    Camk2a[315-478] (Camk2a Rat)
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-NES-YFP-CCTc

    Plasmid

    #184328

    Purpose

    Mammalian expression of the cytosolic localized YFP-CCTd(peptide of Cav1.2)

    Depositor

    Xiaodong Liu

    Article

    Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

    Insert

    CCTc (CACNA1C Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLL7.0: hITSN1(1159-1509)-tgRFPt-SSPB WT

    Plasmid

    #60419

    Purpose

    Mammalian Expression of ITSN DH/PH-tgRFPt-Nano

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    hITSN1-tgRFPt-SSPB (ITSN1 Human, Synthetic)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    tgRFPt

    Expression

    Mammalian

    Mutation

    hITSN 1159-1509 // SSPB Y11K and A15E

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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