We narrowed to 19,551 results for: Cre

1. pLenti6.3delta4 GPR3-V5

   Plasmid

   #172601

   Purpose

   Lentiviral plasmid expressing c-terminal V5-tagged GPR3

   Depositor

   Robert Screaton

   Article

   Iorio et al Commun Biol. 2021 Jul 23;4(1):907. doi:

   Insert

   G protein-Coupled Receptor 3 (GPR3 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. D79_Ld_366740

   Plasmid

   #135892

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   histidine secretory acid phosphatase, putative
   

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Available Since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA- Pconstitutive-sgRNA(Sth3)_cpaA

   Plasmid

   #133348

   Purpose

   expression of two sgRNA from Streptococcus thermophilus #3 each express from its own constitutive promoter; here first one targets blaA and second one targets cpaA (from Caulobacter crescentus)

   Depositor

   Michael Laub

   Article

   Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

   Insert

   sgRNA_blaA and sgRNA_cpaA
   

   Use

   CRISPR

   Promoter

   constitutive

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEMS2023

   Plasmid

   #49130

   Purpose

   AAV plasmid with hs1218 promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-hs1218-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hs1218

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pEMS2024

   Plasmid

   #49131

   Purpose

   AAV plasmid with hs671 promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-hs671-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hs671

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. DCT.VV.Orf232

   Plasmid

   #12920

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jon Yewdell, Jack Bennink

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf232
   

   Expression

   Mammalian

   Available Since

   Dec. 29, 2006

   Availability

   Academic Institutions and Nonprofits only

7. DCT.VV.Orf138

   Plasmid

   #12826

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jon Yewdell, Jack Bennink

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf138
   

   Expression

   Mammalian

   Available Since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

8. DCT.VV.Orf87

   Plasmid

   #12775

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jon Yewdell, Jack Bennink

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf87
   

   Expression

   Mammalian

   Available Since

   Dec. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

9. PET-B-HypaSpCas9-NLS-6xHis

   Plasmid

   #207386

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HypaSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N692A, M694A, Q695A, H698A, amino acids 1005-1013…

   Promoter

   T7

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEMS1994

    Plasmid

    #49124

    Purpose

    AAV plasmid with S100B (Ple266) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple266-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    S100B

    Available Since

    May 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pUC19-dual-scafffold-2.0 (pBP49)

    Plasmid

    #218931

    Purpose

    The dual-scaffold for making dual-CRISPR screening libraries or dual-CRISPR plasmids.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Insert

    dual scaffolds for CRISPR
    

    Use

    CRISPR

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEMS2159

    Plasmid

    #70120

    Purpose

    Promoterless AAV plasmid with MCS to insert promoter for expression of iCre. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-MCS-iCre-WPRE
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    May 12, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pMax_ScaI_BFP

    Plasmid

    #177824

    Purpose

    Site-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    tagBFP
    

    Expression

    Mammalian

    Mutation

    5' inserted ScaI RE cut site

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLVX-TetOne-Puro-EcSTH-FLAG

    Plasmid

    #218628

    Purpose

    This plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    EcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVX-TetOne-Puro-mitoEcSTH-FLAG

    Plasmid

    #218629

    Purpose

    This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

    Depositor

    Valentin Cracan

    Article

    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

    Insert

    mitoEcSTH
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG and MTS

    Expression

    Mammalian

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EF13E

    Plasmid

    #42942

    Depositor

    Eline Luning Prak

    Article

    Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

    Insert

    EN- Human L1 element (L1RE1 Human)
    

    Tags

    EGFP (opposite strand)

    Expression

    Mammalian

    Mutation

    AgeI-BclI fragment swapped with nt 1927-3708 from…

    Promoter

    CMV MIE, L1 5'UTR

    Available Since

    April 25, 2013

    Availability

    Academic Institutions and Nonprofits only

17. PET-B-Sniper SpCas9-NLS-6xHis

    Plasmid

    #207377

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Sniper SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F539S, M763I, K890N, amino acids 1005-1013 replac…

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPapi

    Plasmid

    #96921

    Purpose

    expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.

    Depositor

    David Root, John Doench

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEMS2022

    Plasmid

    #49129

    Purpose

    AAV plasmid with DBH promoter (PRS2/3:Con) driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-PRS2/3:Con-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    DBH

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. PET-Sniper SpCas9-NLS-6xHis

    Plasmid

    #207374

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Sniper SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F539S, M763I, K890N

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge