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We narrowed to 5,170 results for: mos

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Showing: 4041 - 4060 of 5170 results

  1. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid
    #217808
    Purpose
    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217808-plasmid-map-sequence-id-489173

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  2. pLenti GW V5 Eco/Dam hum LaminB1

    Plasmid
    #182671
    Purpose
    Eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin B1. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin B1.
    Depositor
    Bas van Steensel
    Article
    Guelen et al Nature. 2008 Jun 12;453(7197):948-51. do
    Insert
    Lamin B1 (LMNB1 Human)
    Use
    Lentiviral
    Tags
    E. Coli Dam and V5
    Expression
    Mammalian
    Promoter
    HSP
    Available Since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182671-plasmid-map-sequence-id-450317

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  9. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29 (LM2993)

    Plasmid
    #208959
    Purpose
    A variant CE1 construct with ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); ePhi29(-exo)(D169A;M8R/V51A/M97T/…
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208959-plasmid-map-sequence-id-415785

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  10. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid
    #162877
    Purpose
    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM
    Depositor
    Frederic Meunier
    Article
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Insert
    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Insert consists of AA1-170
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162877-plasmid-map-sequence-id-321985

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  12. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid
    #217800
    Purpose
    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217800-plasmid-map-sequence-id-432875

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  19. pmEos2-N1 GBP (GBP-mEos2)

    Plasmid
    #162876
    Purpose
    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy
    Depositor
    Frederic Meunier
    Article
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Insert
    GFP Binding Protein tagged with mEos2
    Tags
    mEos2
    Expression
    Mammalian
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162876-plasmid-map-sequence-id-321989

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Showing: 4041 - 4060 of 5170 results