We narrowed to 6,547 results for: proc

1. mitoTALECD for_atp1_1333CN

   Plasmid

   #186201

   Purpose

   For genome editing (base editing) of Arabidopsis mitochondria, targeted C of RNA editing site of ATP1 by OTP87

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11

   Inserts

   mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
   mitochondrial targeted TALE right hand with 1333B of Cytidine Deaminase (mitoTALECD)
   

   Use

   Synthetic Biology; Ti plasmid for plant transform…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET11-PC(75-228)-H6

   Plasmid

   #183779

   Purpose

   Express PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.

   Depositor

   Peter Harte

   Article

   Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

   Insert

   Polycomb (Pc Fly)
   

   Use

   Tags

   6 His Tag at the C-terminus

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET11-PC(75-390)-H6

   Plasmid

   #183781

   Purpose

   Express PC(75-390)-H6 in E. coli. PC(75-390)-H6 was used for GST-CBP pull-down assays

   Depositor

   Peter Harte

   Article

   Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

   Insert

   Polycomb (w Fly)
   

   Use

   Tags

   6 His tag at the C-terminus

   Expression

   Bacterial

   Mutation

   6 His tag at the C-terminus

   Promoter

   T7

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-PC(1-86)-H6

   Plasmid

   #183780

   Purpose

   Express PC(1-86)-H6 in E. coli. PC(1-86)-H6 was used for GST-CBP pull-down assays

   Depositor

   Peter Harte

   Article

   Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

   Insert

   Polycomb (Pc Fly)
   

   Use

   Tags

   6 His Tag at the C-terminus

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAc5.1B-lambdaN-HA-DmAGO1-K690A_M

   Plasmid

   #146957

   Purpose

   Insect Expression of DmAGO1-K690A

   Depositor

   Elisa Izaurralde

   Article

   Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

   Insert

   DmAGO1-K690A (AGO1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   variation with 2 non silent mutations V315A and H…

   Promoter

   Available since

   Feb. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28_6xHis-PARP1delZn1-L713F

   Plasmid

   #173937

   Purpose

   Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn1-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 1-96; L713F, V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28_6xHis-PARP1delZn3-L713F

   Plasmid

   #173934

   Purpose

   Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn3-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 214-373; L713F, V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET41sIII_GST-ALC1-K77R

   Plasmid

   #174813

   Purpose

   Bacterial expression of inactive ALC1 (K77R disrupts ATP binding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   ALC1-K77R (CHD1L Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   K77R

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET41sIII_GST-ALC1-E175Q

   Plasmid

   #174814

   Purpose

   Bacterial expression of inactive ALC1 (E175Q disrupts ATP binding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   ALC1-E175Q (CHD1L Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   E175Q

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOTTC1535 pscAAV mU6 shRNA(scram) CMV-IE Nuc-EYFP

    Plasmid

    #135563

    Purpose

    An AAV vector expressing scrambled shRNA and a nuclear EYFP reporter

    Depositor

    Christopher Richie

    Article

    Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

    Inserts

    shRNA (scrambled)
    Nuc-EYFP
    

    Use

    AAV and RNAi

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE and mU6

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. deltaLoop-MIDAS

    Plasmid

    #171605

    Purpose

    Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.

    Depositor

    Tarun Kapoor

    Article

    Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

    Insert

    MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
    

    Use

    Tags

    His6 tag and SNAP tag

    Expression

    Bacterial

    Mutation

    Replaced amino acids 4458-4496 with ASGSGS linker

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pInducer10-UBC9-sh3

    Plasmid

    #168987

    Purpose

    Expresses RFP cDNA and UBC9 shRNA 3 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Ubiquitin Conjugating Enzyme 9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHis-Hgd

    Plasmid

    #163091

    Purpose

    Expressing mouse Hgd in bacterial cells

    Depositor

    Joseph Takahashi

    Article

    Yang et al Proc Natl Acad Sci U S A. 2019 Oct 29;11

    Insert

    Hgd (Hgd Mouse)
    

    Use

    Tags

    his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. QuasAr-P2A-hKvb1

    Plasmid

    #154083

    Purpose

    Independent expression of QuasAr and human Kvbeta 1 in pFCK vector

    Depositor

    Michael Hoppa

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 Nov 24;11

    Insert

    QuasAr-P2A-human Kvb1 (KCNAB1 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    myc-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3/flag-pBirA(1-K283)-FRB

    Plasmid

    #154451

    Purpose

    expresses flag-pBirA(1-K283)-FRB/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    flag-pBirA(1-K283)-FRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-XYLT2-sgRNA

    Plasmid

    #154862

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting XYLT2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    XYLT2 sgRNA (XYLT2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-myc-H2-Ld-VN

    Plasmid

    #135520

    Purpose

    Mammalian expression of VN-fused and myc-tagged H2-Ld

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Ld
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. hsa-cdk2 3'UTR sicheck2 mut

    Plasmid

    #121124

    Purpose

    luciferase assay

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    hsa-cdk2 3'UTR mutated 199 binding (CDK2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    miR-199 seed binding site mutated

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tol2-lyzC-mcherry-2a-CDK2-D145N

    Plasmid

    #121132

    Purpose

    neutrophil specific expression

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    dre-D145N CDK2 DN (cdk2 Zebrafish)
    

    Use

    Tags

    mcherry-2a

    Expression

    Bacterial

    Mutation

    D145N point mutation DN

    Promoter

    neutrophil specific lyzC

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. dre-cdk2 3'UTR sicheck2 199 mut

    Plasmid

    #121122

    Purpose

    luciferase assay

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    dre-cdk2 3'UTR mutated 199 binding (cdk2 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    miR-199 seed binding site mutated

    Promoter

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge