Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 22,077 results for: his

Showing: 6091 - 6120 of 22077 results

  1. pEW107

    Plasmid
    #232796
    Purpose
    COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) in pBIG2ab
    Depositor
    Cynthia Wolberger
    Article
    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1
    Insert
    COMPASS Fragment 2 (His-FLAG-ySet1, Cps30, Twinstrep-Cps40) (SET1, SPP1, SWD3 Budding Yeast)
    Use
    Tags
    His6-3xFLAG, Twinstrep
    Expression
    Insect
    Mutation
    ySet1(762-1080)
    Promoter
    polyhedrin promoter
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    232796-plasmid-map-sequence-id-465733

    Enlarge

  3. eSrtA-MT4

    Plasmid
    #200306
    Purpose
    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    eSrtA-LPETG-MT4 (MT4 Human)
    Use
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    200306-plasmid-map-sequence-id-394863

    Enlarge

  4. eSrtA-MT3

    Plasmid
    #200305
    Purpose
    Bacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT3. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtA
    Depositor
    Artur Krężel
    Article
    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.
    Insert
    eSrtA-LPETG-MT3 (MT3 Human)
    Use
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    200305-plasmid-map-sequence-id-394866

    Enlarge

  12. pBEL2265

    Plasmid
    #175962
    Purpose
    For expresson of LetA-[LetB PLL3->6] operon from E. coli. N-terminal his tag on LetA, araBAD promoter
    Depositor
    Damian Ekiert, Gira Bhabha
    Article
    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d
    Inserts
    LetA
    LetB
    Use
    Tags
    8xHis 2xQH and TEV cleavage site on LetA
    Expression
    Bacterial
    Mutation
    Δ347-361, replace with 702-716
    Promoter
    araBAD
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    175962-plasmid-map-sequence-id-345244

    Enlarge

  28. pBad-CA TAG186

    Plasmid
    #105837
    Purpose
    Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 186 and C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 186
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105837-plasmid-map-sequence-id-205518

    Enlarge

  29. pBad-CAts TAG97

    Plasmid
    #105668
    Purpose
    expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 97 and C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 97
    Promoter
    pBad/arabinose
    Available since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105668-plasmid-map-sequence-id-205515

    Enlarge

Showing: 6091 - 6120 of 22077 results