We narrowed to 87,502 results for: lias

1. pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9

   Plasmid

   #84242

   Purpose

   Expresses GA-inducible KRAB-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sp dCas9
   GID1-KRAB
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and PGK

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Human FLAG-WDR20

   Plasmid

   #217618

   Purpose

   Mammalian expression of FLAG tagged WDR20

   Depositor

   Ethan Lee

   Article

   Ng et al Nat Commun. 2023 Oct 5;14(1):6173. doi:

   Insert

   WDR20 (WDR20 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-KLF3

   Plasmid

   #49100

   Purpose

   Expresses KLF3 in mammalian cells

   Depositor

   Merlin Crossley

   Article

   Bell-Anderson et al Diabetes. 2013 Aug;62(8):2728-37. doi: 1

   Insert

   Krüppel-Like Factor 3(KLF3)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pLVX-Puro-TDP-43-D169G

   Plasmid

   #133757

   Purpose

   Lentiviral expression of human TDP-43 D169G disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed aspartate 169 to glycine.

   Promoter

   CMV

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET48 AtGUN1-SMR1

   Plasmid

   #136361

   Purpose

   Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 709 to 918)

   Depositor

   Tatsuru Masuda

   Article

   Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

   Insert

   cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 709 to 918 (GUN1 Mustard Weed)
   

   Use

   Tags

   TRX-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET48 AtGUN1-SMR2

   Plasmid

   #136362

   Purpose

   Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 781 to 918)

   Depositor

   Tatsuru Masuda

   Article

   Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

   Insert

   cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 781 to 918 (GUN1 Mustard Weed)
   

   Use

   Tags

   TRX-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET48 AtGUN1-PPR1

   Plasmid

   #136359

   Purpose

   Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)

   Depositor

   Tatsuru Masuda

   Article

   Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

   Insert

   cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708 (GUN1 Mustard Weed)
   

   Use

   Tags

   TRX-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET48 AtGUN1-PPR2

   Plasmid

   #136360

   Purpose

   Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 619)

   Depositor

   Tatsuru Masuda

   Article

   Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

   Insert

   cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 619 (GUN1 Mustard Weed)
   

   Use

   Tags

   TRX-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcsII-pb6-flag

   Plasmid

   #86770

   Purpose

   C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   proteasome beta subunit 6 (PSMB6 Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   unknown

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAL130

    Plasmid

    #22475

    Purpose

    Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF3NT (PIF3 Mouse, Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBp-FGFR2b-K660E

    Plasmid

    #45705

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIb (FGFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K660E

    Promoter

    Available since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

12. LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid

    #47455

    Purpose

    LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    NLS(alpha-imp)-CRY2PHR-NLS-VP64
    

    Use

    AAV

    Tags

    2A_GFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn human synapsin (mammalian neuronal expressio…

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1+-PAFAH1B1_R22-

    Plasmid

    #92431

    Purpose

    mammalian expression of PAFAH1B1 R22 mutant. The protein is truncated and non functional (negative control).

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

    Insert

    PAFAH1B1 (PAFAH1B1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R22 (stop codon in position 22)

    Promoter

    CMV

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBp-FGFR3c-S249C

    Plasmid

    #45713

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR3 IIIc (FGFR3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    S249C

    Promoter

    Available since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pCMV-BEACON1

    Plasmid

    #171697

    Purpose

    Expresses BEACON1 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Cell Rep. 2020 Jun 2;31(9):107723. doi:

    Insert

    BEACON1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_W104A/Y132D

    Promoter

    CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTE4254

    Plasmid

    #89050

    Purpose

    Expresses St chimeric gRNA and human codon-optimized StCas9

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    St chimeric gRNA
    hStCas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA4TO-3XFLAG Halo K164A/H165A nsp1

    Plasmid

    #175421

    Purpose

    Expresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJZC25

    Plasmid

    #62328

    Purpose

    sgRNA + 1x MS2 with MCP-VP64 effector for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 1x MS2 binding module
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBp-FGFR2b-K660N

    Plasmid

    #45707

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIb (FGFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K660N

    Promoter

    Available since

    June 24, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pAAV-CAG-dLight1.2

    Plasmid

    #111069

    Purpose

    To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Has Service

    AAV1

    Insert

    dLight1.2
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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