We narrowed to 7,491 results for: RAP

Showing: 6121 - 6150 of 7491 results

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pTT021

Plasmid

#226782

Purpose

Expression of wzxE and other genes from the E. coli enterobacterial common antigen biosynthesis gene cluster

Depositor

James Naismith, Chris Whitfield

Article

Le Bas et al Open Biol. 2025 Jan;15(1):240310. doi: 1

Insert

wzxE, wecF, wzyE, wecG

Expression

Bacterial

Promoter

BAD

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pC130: pAAV.CMV-CasRx-VEGFA sgRNA

Plasmid

#203443

Purpose

Plasmid expressing active RfxCas13d with VEGFA mRNA targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA
RfxCas13d

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pM148: pAAV.CMV-Cas13e-NT sgRNA

Plasmid

#203446

Purpose

Plasmid expressing active RfxCas13d with non-targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-non targeting (NT) sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pM149: pAAV.CMV-Cas13e-VEGFA sgRNA

Plasmid

#203447

Purpose

Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pM150: pAAV.CMV-Cas13e-VEGFA sgRNA array

Plasmid

#203448

Purpose

Plasmid expressing active Cas13e with an array of three VEGFA mRNA targeting gRNAs

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA array
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pM155: pAAV.CMV-Cas13e (GenScript CO)-NT sgRNA

Plasmid

#203451

Purpose

Plasmid expressing active and codon optimised Cas13e with non-targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-non targeting (NT) sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Mutation

Codon optimised using GenScript tool

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pM156: pAAV.CMV-Cas13e (GenScript CO)-VEGFA sgRNA

Plasmid

#203452

Purpose

Plasmid expressing active and codon optimised Cas13e with VEGFA gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1301-AAV-EFSNC-dCjCas9-NIPP1(143-224)

Plasmid

#223146

Purpose

Expression of truncated NIPP1 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

NIPP1 (PPP1R8 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 143-224

Promoter

EF1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1300-AAV-EFSNC-dCjCas9-MBD2(176-231)

Plasmid

#223145

Purpose

Expression of truncated MBD2 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MBD2 (MBD2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 176-231

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Tnnt2-Cre-U6-Lmna-sgRNA

Plasmid

#206178

Purpose

Expresses Cre recombinase specifically in cardiomyocytes and uses U6 promoter to express sgRNAs targeting murine Lmna exon 10

Depositor

Yuxuan Guo

Article

Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

Insert

Cre

Use

AAV

Promoter

cTnT

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1311-AAV-EFSNC-dSaCas9-NIPP1(143-224)

Plasmid

#223156

Purpose

Expression of truncated NIPP1 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

NIPP1 (PPP1R8 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 143-224

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1310-AAV-EFSNC-dSaCas9-MBD2(176-231)

Plasmid

#223155

Purpose

Expression of truncated MBD2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MBD2 (MBD2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 176-231

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-MCHpr-DREADD (Gq)-mCherry

Plasmid

#221036

Purpose

Expresses Gq coupled DREADD hM3D under the rat pro-melanin-concentrating hormone promoter

Depositor

Yanhua Huang

Article

Wang et al Mol Psychiatry. 2021 Jul;26(7):3152-3168

Insert

DREADD Gq-mCherry

Use

AAV; Rat targeting

Expression

Mammalian

Promoter

Rat MCH

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_DAZL_cterm2

Plasmid

#222919

Purpose

Cas9/sgRNA plasmid for targeting DAZL

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, DAZL sgRNA 2 (DAZL Human, Synthetic)

Use

CRISPR

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_DAZL_cterm3

Plasmid

#222920

Purpose

Cas9/sgRNA plasmid for targeting DAZL

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, DAZL sgRNA 3 (DAZL Human, Synthetic)

Use

CRISPR

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_TFAP2C_cterm

Plasmid

#222916

Purpose

Cas9/sgRNA plasmid for targeting TFAP2C

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, TFAP2C sgRNA (TFAP2C Human, Synthetic)

Use

CRISPR

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_FIGLA_cterm4

Plasmid

#222909

Purpose

Cas9/sgRNA plasmid for targeting FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, FIGLA sgRNA 4 (FIGLA Human, Synthetic)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_FIGLA_cterm3

Plasmid

#222908

Purpose

Cas9/sgRNA plasmid for targeting FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, FIGLA sgRNA 3 (FIGLA Human, Synthetic)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_FIGLA_cterm2

Plasmid

#222907

Purpose

Cas9/sgRNA plasmid for targeting FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, FIGLA sgRNA 2 (FIGLA Human, Synthetic)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_FIGLA_cterm1

Plasmid

#222906

Purpose

Cas9/sgRNA plasmid for targeting FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, FIGLA sgRNA 1 (FIGLA Human, Synthetic)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NANOS3_cterm

Plasmid

#222904

Purpose

Cas9/sgRNA plasmid for targeting NANOS3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NANOS3 sgRNA (NANOS3 Human, Synthetic)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-FIGLA

Plasmid

#222929

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

FIGLA (FIGLA Human)

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DLX5

Plasmid

#222927

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DLX5

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

DLX5 (DLX5 Human)

Expression

Mammalian

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-LHX8

Plasmid

#222921

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of LHX8

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

LHX8 (LHX8 Human)

Expression

Mammalian

Available Since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-CCND1shRNA3

Plasmid

#220579

Purpose

To inducibly knockdown CCND1 expression

Depositor

Sean Lee

Article

Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

Insert

CCND1 shRNA3 (CCND1 Human)

Use

Lentiviral

Promoter

TRE3G (TetOP) promoter

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH

Plasmid

#131684

Purpose

An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1

Depositor

Christopher Richie

Article

Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

Inserts

EGFP-KASH
SpCas9 sgRNA vs mouse GRID1

Use

AAV

Tags

KASH

Promoter

EF1a and mU6-LSL (Cre dependent)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA320

Plasmid

#215952

Purpose

Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

rev{U6_v1; DR_v0 [EnAs]; sgCD47_v1 [EnAs]; DR_v1 [EnAs]; sgCD47_v2 [EnAs]}

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA315

Plasmid

#215951

Purpose

Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control lethal

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

rev{U6_v1; DR_v0 [EnAs]; sgPLK1_v1 [EnAs]; DR_v1 [EnAs]; sgPLK1_v2 [EnAs]}

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA074

Plasmid

#215933

Purpose

Fragmid fragment: (guide cassette) Tet-regulatable guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

H1-2xTetO_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA435

Plasmid

#215962

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; DR_v0 [EnAs]; sgCD274_v1; DR_v1 [EnAs]; sgADGRE5_v3; DR_v2; sgCD4_v1; DR_v3; sgDPP4_v1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge