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We narrowed to 8,240 results for: FIE

Showing: 8161 - 8200 of 8240 results

  1. YFP(1-158)-beta-5 in pcDNAI/Amp

    Plasmid
    #54470
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-5. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Insert
    YFP(1-158)/beta-5 (GNB5 Human, Aequorea victoria)
    Use
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    YFP (1-158) includes a substitution of Met for Gl…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54470-plasmid-map-sequence-id-114865

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  2. CFP(159-238)-beta-1 in pcDNAI/Amp

    Plasmid
    #55592
    Purpose
    A carboxyl-terminal CFP fragment was fused to Gbeta-1. When co-expressed with an amino-trerminal CFP or YFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    CFP (159-238)-Beta 1 (GNB1 Human, Aequorea victoria)
    Use
    Tags
    CFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    CFP(159-238) includes a substitution of His for A…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55592-plasmid-map-sequence-id-114863

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  3. pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

    Plasmid
    #213030
    Purpose
    Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    213030-plasmid-map-sequence-id-422062

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  6. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid
    #213026
    Purpose
    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213026-plasmid-map-sequence-id-422069

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  9. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

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  10. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213027
    Purpose
    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    D702Y (RT- reverse transcriptase catalytic dead)
    Promoter
    CMV
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213027-plasmid-map-sequence-id-452358

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  11. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213029
    Purpose
    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    Use
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213029-plasmid-map-sequence-id-422050

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  12. pFBOH-LIC_SMU1:1-513

    Plasmid
    #210877
    Purpose
    Insect expression of full-length human SMU1. N-terminal 6X His tag with thrombin protease cleavage site.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    SMU1:M1-P513 (SMU1 Human)
    Use
    Tags
    MGSSHHHHHHSSGLVPRGS
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 19, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210877-plasmid-map-sequence-id-426048

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  16. pFBOH-MHL_SHKBP1:182-585

    Plasmid
    #210935
    Purpose
    Insect expression of human SHKBP1 fragment T182 to L585. N-terminal 6X His tag with TEV cleavage.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    SHKBP1:T182-L585 (SHKBP1 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Insect
    Mutation
    Q507L natural variant (VAR_036714)
    Promoter
    Polyhedrin
    Available since
    March 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210935-plasmid-map-sequence-id-426241

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  24. pFBOH-MHL_WDR13:142-485

    Plasmid
    #210924
    Purpose
    Insect expression of human WDR13 fragment A142 to K485. N-terminal 6X His tag with TEV cleavage.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    WDR13:A142-K485 (WDR13 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Insect
    Mutation
    H325R natural variant (VAR_060284)
    Promoter
    Polyhedrin
    Available since
    March 20, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210924-plasmid-map-sequence-id-426206

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  29. pFBOH-MHL_WDR12:90-417

    Plasmid
    #210903
    Purpose
    Insect expression of human WDR12 fragment T90 to T417. N-terminal 6X His tag with TEV cleavage.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    WDR12:T90-T417 (WDR12 Human)
    Use
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Insect
    Mutation
    introduced mutations F99A K131A R319S
    Promoter
    Polyhedrin
    Available since
    March 19, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210903-plasmid-map-sequence-id-426133

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  36. pFBD-BirA_WDR12:90-422

    Plasmid
    #210893
    Purpose
    Insect expression of human WDR12 fragment T90 to G422. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    WDR12:T90-G422 (WDR12 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210893-plasmid-map-sequence-id-424521

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  37. pFBD-BirA_WDR41:30-459

    Plasmid
    #210892
    Purpose
    Insect expression of human WDR41 fragment Q30 to V459. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    WDR41:Q30-V459 (WDR41 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210892-plasmid-map-sequence-id-424520

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  38. pFBD-BirA_WDR91:1-747

    Plasmid
    #210879
    Purpose
    Insect expression of full-length human WDR91. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    WDR91:M1-A747 (WDR91 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210879-plasmid-map-sequence-id-424496

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  39. pFBD-BirA_RACK1:1-317

    Plasmid
    #210889
    Purpose
    Insect expression of full-length human RACK1. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    RACK1:M1-R317 (RACK1 Human)
    Use
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Insect
    Mutation
    wild type
    Promoter
    Polyhedrin
    Available since
    March 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    210889-plasmid-map-sequence-id-424503

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Showing: 8161 - 8200 of 8240 results