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We narrowed to 6,952 results for: CAR

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Showing: 4081 - 4100 of 6952 results

  5. pETSUMO2_Vitiosangium-bGSDM-thrombin

    Plasmid
    #222099
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Publication
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Vitiosangium-bGSDM-thrombin ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222099-plasmid-map-sequence-id-437094

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  6. Ad:2CMV_A2R2-N25Ctr

    Plasmid
    #122243
    Purpose
    Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone
    Depositor
    Corey Neu
    Publication
    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516
    Inserts
    C-terminal end of Nesprin-3 w/o Kash (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    Use
    Adenoviral
    Tags
    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…
    Expression
    Mammalian
    Mutation
    deleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…
    Promoter
    CMV
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    122243-plasmid-map-sequence-id-239188

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  8. pEcRNAP5

    Plasmid
    #128939
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, Z
    Depositor
    Seth Darst
    Publication
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoAppx234-241-(His)10 (rpoA Escherichia coli)
    RpoB
    RpoC
    RpoZ
    Tags
    His
    Expression
    Bacterial
    Mutation
    Residues 234-241 are substituted for a PreScissio…
    Promoter
    T7
    Available since
    Aug. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    128939-plasmid-map-sequence-id-254599

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  10. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

    Plasmid
    #119864
    Purpose
    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d
    Insert
    BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    Tags
    HA-tagged biotin ligase (BirA), Thosea asigna vir…
    Expression
    Bacterial
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    119864-plasmid-map-sequence-id-239418

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  11. pGEX-6P-3-F37A+L196F+V252A+C253G-hADPRM

    Plasmid
    #107163
    Purpose
    Expresses specific cyclic-ADP-ribose (cADPR) phosphohydrolase in Escherichia coli as a GST fusion protein that can be cut with PreScission(TM) protease to yield active enzyme
    Depositor
    Jose Carlos Cameselle
    Publication
    Ribeiro et al Sci Rep. 2018 Jan 18;8(1):1036. doi: 10.
    Insert
    F37A+L196F+V252A+C253G-hADPRM GST-mutant cADPR phosphohydrolase gene
    Tags
    GST
    Expression
    Bacterial
    Promoter
    tac
    Available since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107163-plasmid-map-sequence-id-204806

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  18. GRM1

    Plasmid
    #25353
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GRM1-C254S:L28-G518 (GRM1 Human)
    Tags
    His
    Expression
    Insect
    Mutation
    C254S. Contains amino acids L28-G518.
    Available since
    July 29, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. SOX2-P2A-tagBFP-HDR

    Plasmid
    #163751
    Purpose
    HDR knock-in template for tagging the human endogenous SOX2 gene with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.
    Depositor
    Jennifer Mitchell
    Publication
    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.
    Insert
    SOX2 (SOX2 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    P2A-tagBFP
    Mutation
    Does not contain start codon to avoid random inte…
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    163751-plasmid-map-sequence-id-326711

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Showing: 4081 - 4100 of 6952 results