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We narrowed to 7,654 results for: Pol

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Showing: 4081 - 4100 of 7654 results

  1. PX459v3-eSpCas9(1.1)

    Plasmid
    #178800
    Purpose
    High-fidelity eSpCas9(1.1) with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178800-plasmid-map-sequence-id-354839

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  2. PX459v3-SpCas9-HF1

    Plasmid
    #178801
    Purpose
    High-fidelity SpCas9-HF1 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178801-plasmid-map-sequence-id-354837

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  5. pCAGGS-N-TEV-HAT

    Plasmid
    #202598
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell
    Depositor
    Chiaki Murakami
    Publication
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Type
    Empty backbone
    Tags
    TEV cleavable site (ENLYFQG) and poly-histidine a…
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    202598-plasmid-map-sequence-id-399468

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  6. pT7-V5-SBP-C1-HshnRNPE2iso3_Z

    Plasmid
    #148140
    Purpose
    Mammalian Expression of HshnRNPE2iso3
    Depositor
    Elisa Izaurralde
    Insert
    HshnRNPE2iso3 (PCBP2 Human)
    Expression
    Mammalian
    Mutation
    one non silent mutation L239 to S compapred to th…
    Available since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148140-plasmid-map-sequence-id-385303

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  8. pET26b(+)-ATI_QconCAT_1

    Plasmid
    #163957
    Purpose
    Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.
    Depositor
    Stefan Tenzer
    Publication
    Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102
    Insert
    ATI QconCAT
    Tags
    polyhistidine tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    163957-plasmid-map-sequence-id-321834

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  9. tet-pLKO.puro_shHuR 3UTR

    Plasmid
    #110412
    Purpose
    TRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAVL1 (HuR)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    H1/TO (RNA PolIII)
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110412-plasmid-map-sequence-id-213309

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  10. pCJ199

    Plasmid
    #162672
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162672-plasmid-map-sequence-id-321466

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  12. pJin-278

    Plasmid
    #125714
    Purpose
    ABA-inducible split T7 GFP vector using RNAPN d5-19
    Depositor
    Bryan Dickinson
    Publication
    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PT7-GFP mRNA expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
    Expression
    Mammalian
    Available since
    May 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    125714-plasmid-map-sequence-id-244341

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  13. 5THA (Gemin5)

    Plasmid
    #101890
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Gemin5 (GEMIN5 Human)
    Use
    Baculovirus expression
    Tags
    6xHis, Thrombin cleavage
    Promoter
    polyhedrin
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    101890-plasmid-map-sequence-id-194723

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  16. pMpGWB218

    Plasmid
    #68609
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Publication
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Promoter
    MpEF1alpha promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68609-plasmid-map-sequence-id-121811

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Showing: 4081 - 4100 of 7654 results