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C1-MPAct-mCitrine

Plasmid

#155220

Purpose

Monitor changes in the density of membrane-proximal F-actin (MPA)

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b

Tags

mCitrine

Expression

Mammalian

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3A WT

Plasmid

#123089

Purpose

Expresses 3xFLAG-LC3A wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pTol2-UAS-zArchon1-KGC-EGFP-ER2

Plasmid

#108427

Purpose

Codon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

zArchon1-KGC-EGFP-ER2

Use

Zebrafish

Promoter

UAS

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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MAP2-GCaMP3

Plasmid

#43917

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Insert

GCaMP3

Use

Lentiviral

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

E247V in GCaMP3

Promoter

Human MAP2 promoter

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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783 pCScherryActMEK

Plasmid

#31880

Depositor

Nathan Lawson

Article

Covassin et al Dev Biol. 2009 May 15. 329(2):212-26.

Insert

MEK1 (MAP2K1 Human)

Use

Expression vector

Tags

mCherry

Mutation

Ser218Glu Ser222Asp; activated MEKK

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-ABEmax-NRCH

Plasmid

#136923

Purpose

Expresses ABEmax-NRCH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

ABEmax-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

Plasmid

#132400

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H151A; H1121A

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-KRAS-G12V

Plasmid

#116424

Purpose

Lentiviral expression of KRAS G12V

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

KRAS (KRAS Human)

Use

Lentiviral

Mutation

G12V

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCLXEBR-pTF-cKit

Plasmid

#114293

Purpose

TET-inducible expression of c-Kit

Depositor

Patrick Salmon

Article

Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

Insert

cKit (Kit Mouse)

Use

Lentiviral

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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MEK1 (S218D,S222D)-pcw107

Plasmid

#64604

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Mutation

S218D, S222D

Promoter

PGK

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFOK

Plasmid

#166651

Purpose

Suicide vector for gene manipulation

Depositor

Dirk Bumann

Article

Cianfanelli et al BMC Microbiol. 2020 May 24;20(1):129. do

Insert

I-sceI, sacB

Use

Synthetic Biology

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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p5E-4xnrUAS (L4-R1)

Plasmid

#61372

Purpose

5' Gateway Entry Clone Containing 4xnrUAS

Depositor

Marnie Halpern

Article

Akitake et al Dev Biol. 2011 Apr 15;352(2):191-201. do

Insert

4xnrUAS

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pOSIP-KC (KanR, PhiC31)

Plasmid

#45982

Depositor

Drew Endy, Keith Shearwin

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pT-GEM(0)

Plasmid

#62891

Purpose

Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Type

Empty backbone

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pVITRO1-102.1F10-IgG3/λ

Plasmid

#50368

Purpose

Expresses grass pollen allergen Phl p 7 specific human IgG3/λ antibody isotype (102.1F10-IgG3/λ)

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

grass pollen allergen Phl p 7 specific human Gamma 3 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette

Expression

Mammalian

Promoter

Mouse Elongation Factor 1 Alpha and Rat Elongatio…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcdna6.2- myc ULK1 4SA

Plasmid

#27631

Depositor

Reuben Shaw

Article

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 4SA (Myc Mouse)

Tags

myc

Expression

Mammalian

Mutation

S467A, S555A, T574A, S637A. Initial M from ULK1 r…

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

Plasmid

#172506

Purpose

Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 bacterial expression vector with golden gate site for guides

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP2

Plasmid

#17793

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pUC8-SZ

Plasmid

#17330

Depositor

Takeshi Sano

Article

Argarana et al Nucleic Acids Res. 1986 Feb 25. 14(4):18

Insert

streptavidin

Use

For maintenance

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
plenti-CAG-IRF4-FLAG-IRES-GFP

Plasmid

#107389

Purpose

Lentiviral expression of IRF4-FLAG

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IRF4 (IRF4 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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