We narrowed to 10,863 results for: HA
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Plasmid#51056PurposeExpress self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.DepositorInsertRGR-GFP
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterYeast ADH1 promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-3XHA
Plasmid#127881PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter HA taggingDepositorAvailable SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
ibpAB-GFP
Plasmid#109390PurposeGFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertpibpAB-GFP
UseTagsExpressionBacterialMutationPromoterAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-TERF1
Plasmid#222542PurposePiggyBac transposon plasmid for doxycycline inducible expression of TERF1DepositorAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+_HA-mAID-nanobody (Kless)
Plasmid#117718PurposemRNA synthesis of lysine-less HA-mAID-nanobdy for injectionDepositorInsertmAID-nanobody
UseTags3xHAExpressionMammalianMutationcodon-optimized and lysine-less mAID for murine e…PromoterSP6Available SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
htpG1-GFP
Plasmid#109387PurposeGFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG1-GFP
UseTagsExpressionBacterialMutationPromoterAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pM122: pAAV-EFS-CasRx
Plasmid#166870PurposeAAV vector for expressing CasRxDepositorInsertRfxCas13d
UseAAV and CRISPRTagsHA and NLSExpressionMammalianMutationPromoterEFSAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYSD313
Plasmid#212931PurposeEncodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.TBG mCherry-LDTS-eLIR
Plasmid#189002Purposelipophagy-inducing adaptor protein, AAVDepositorInsertLDTS-eLIR, mCherry
UseAAVTagsHAExpressionMutationPromoterAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
htpG2-GFP
Plasmid#109388PurposeGFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertphtpG2-GFP
UseTagsExpressionBacterialMutationPromoterAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
epB_UbC_tdMS2cp-tagRFP-Tx2
Plasmid#119908PurposeePiggyBac mammalian expression plasmid for tandem dimer MS2cp-tagRFP-T fusionDepositorInsertHA-tdMS2cp-tagRFP-Tx2-nls
UseTagsExpressionMammalianMutationPromoterAvailable SinceJuly 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
HA.PKD.K/W
Plasmid#10809DepositorInsertPKD (PRKD1 Human)
UseTagsHA and Myc (inserted between amino acids 33&3…ExpressionMammalianMutationKinase dead mutant: K612W (Lys 612 to Trp) (bp183…PromoterCMVAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK7 K222R
Plasmid#80887Purposemammalian expression of ALK7 K222RDepositorAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
HA.PKD.S738E/S742E
Plasmid#10810DepositorInsertPKD (PRKD1 Human)
UseTagsHAExpressionMammalianMutationActivation loop active: S738E and S742E (bp 2212-…PromoterCMVAvailable SinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
FLAG-TKGAPDH in pLJM60
Plasmid#154217PurposeLentiviral vector for stable expression of FLAG-TK-GAPDHDepositorInsertGAPDH
UseLentiviralTagsFLAGExpressionMammalianMutationcDNA has been codon optimized for human cell expr…PromoterCMVAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
GreenT-EC - GPI
Plasmid#193946PurposeMammalian expression vector for a low affinity calcium indicator exposed to the cell surfaceDepositorInsertGreenT-EC
UseTagsGPI-anchor and Igk_secretion - HA-FlagExpressionMammalianMutationPromoterAvailable SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSH1/M-FRBl2-E
Plasmid#15289DepositorInsertFRAP-rapamycin binding domain (MTOR Human)
UseTagsHA epitope and Myristoylation-targeting domain c-…ExpressionMammalianMutationres. 2025-2113, T2098LPromoterAvailable SinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCsC_spacer
Plasmid#136077PurposeUse when making odd level constructs (L3,...) if pCsC position (position 3) has no construct assignedDepositorInsertEven spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCsE_spacer
Plasmid#136078PurposeUse when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assignedDepositorInsertEven spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLNC Wnt-3aHA
Plasmid#18030DepositorAvailable SinceJuly 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt(K179M)
Plasmid#39535DepositorInsertAkt(K179M) (Akt1 Mouse)
UseTags6G linker, EGFP, and HAExpressionMammalianMutationK179MPromoterAvailable SinceSept. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCB-hDot1Lmut
Plasmid#74174PurposeFor packaging hDot1Lmut (catalytic activity dead) to retrovirus.DepositorInserthDot1L (DOT1L Human)
UseRetroviralTagsHAExpressionMutationhDot1L 163 GSG to RCR mutation*PromoterAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGinB-8X(GGS)-dCas9-FLAG-NLS
Plasmid#81205PurposeCMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminusDepositorInsertpGinB-8X(GGS)-dCas9-FLAG-NLS
UseTagsExpressionMammalianMutationPromoterCMVAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA(MS2)-puro-barcode
Plasmid#89493PurposeCloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seqDepositorInsertsgRNA (with MS2 loop)
UseLentiviralTagsExpressionMutationPromoterU6Available SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2-sod1
Plasmid#107594PurposeContains zebrafish sod1 gene in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.DepositorAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-5INS_16
Plasmid#102292PurposeYeast Surface Display of 5Ins_16 scFVDepositorInsertInsulin binding scFV
UseTags3x(G4S) Linker, G3S Linker, HA, and MycExpressionYeastMutationPromoterGAL1Available SinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMS158
Plasmid#215679PurposeEmpty repair plasmid for ChrIII split hygromycinR landing padDepositorInsert5'HA + MCS + lox2272 + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxTagsExpressionWormMutation5' ∆HYGR encodes aa1-226PromoterAvailable SinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xHA-BioID_pAS28
Plasmid#118223Purposeexpresses 3xHA-tagged BioID in C. elegans intestineDepositorInsertBioID (BirA mutant)
UseTags3x HA tagExpressionWormMutationR118GPromoterges-1pAvailable SinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW
Plasmid#133359PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
PROM_MpUBE2
Plasmid#136109PurposePROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.DepositorInsertPROM_MpUBE2 Mapoly0106s0019
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
5UTR_MpUBE2
Plasmid#136110Purpose5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.DepositorInsert5UTR_MpUBE2 Mapoly0106s0019
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNoTag-DGCR8
Plasmid#164694PurposeExpress DGCR8 without tag in mammalian cellsDepositorAvailable SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
TAL-L1-1.3-Ctrl
Plasmid#102355PurposePlasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning siteDepositorInsertTAL-L1-1.3
UseT3 in vitro transcription for expression in mamma…TagsFLAG and HAExpressionMutationPromoterAvailable SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE Hygro ST6GalNaC5
Plasmid#26981DepositorAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-5INS_EV3
Plasmid#102293PurposeYeast Surface Display of 5Ins_16_EV3DepositorInsertEvolved Insulin binding scFV
UseTags3x(G4S) Linker, G3S Linker, HA, and MycExpressionYeastMutationPromoterGAL1Available SinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCk1_spacer
Plasmid#136071PurposeUse when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assignedDepositorInsertOdd spacer
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMV+ plasmid
Plasmid#169740PurposeUnregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
p35S-PBE
Plasmid#119772PurposeConversion of C to T in potatoDepositorInsertNLS-APOBEC1-nCas9-UGI-NLS
UseTagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9PromoterCaMv 2x35SAvailable SinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
TAL-L1-1.3-VP64
Plasmid#103033PurposePlasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by VP64 activatorDepositorInsertTAL-L1-1.3-VP64
UseT3 in vitro transcription for expression in mamma…TagsHA and VP64ExpressionMutationPromoterAvailable SinceNov. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GoldenGate
Plasmid#47388PurposeExpression plasmid for TALE proteins compatible with GoldenGate cloning systemDepositorTypeEmpty backboneUseTALENTagsFlag, HA, and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only