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BB3rN_AF

Plasmid

#98554

Purpose

empty BB3 for insertion and overexpression of 5 genes in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB3rN_AG

Plasmid

#98555

Purpose

empty BB3 for insertion and overexpression of 6 genes in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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BB3rN_AI

Plasmid

#98557

Purpose

empty BB3 for insertion and overexpression of 8 genes in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-GFP-HA no-pA

Plasmid

#82589

Purpose

3' entry vector containing HA epitope fused to C-terminus of GFP without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

GFP-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pPac-PL-mCD8-Gal4

Plasmid

#39456

Depositor

Benjamin White

Article

Diao et al Genetics. 2012 Mar;190(3):1139-44. Epub

Insert

mCD8-Gal4

Use

S2

Promoter

actin

Available Since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pBS-F1-TGT

Plasmid

#30711

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 TGT

Use

Cloning vector

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pBS-F2-CAG

Plasmid

#30717

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F2 CAG

Use

Cloning vector

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pBS-F3-GCC

Plasmid

#30740

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F3 GCC

Use

Cloning vector

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pBS-F3-GCG

Plasmid

#30751

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F3 GCG

Use

Cloning vector

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pBS-F1-GAT

Plasmid

#30753

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GAT

Use

Cloning vector

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pBS-F2-AGA

Plasmid

#30756

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F2 AGA

Use

Cloning vector

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pBS-F3-AGA

Plasmid

#30757

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F3 AGA

Use

Cloning vector

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pEMY01AB-PRO

Plasmid

#127714

Purpose

Integrative yeast promoter characterization plasmid. Clone in with BpiI. Has ChrXV homology upstream and an mVenus fragment downstream.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pEMY01CD-TER

Plasmid

#127751

Purpose

Integrative yeast terminator characterization plasmid. Clone in with BpiI. Has mVenus fragment upstream and a KanMX fragment downstream.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pCK693

Plasmid

#242911

Purpose

Constitutive expression of dCas9 and MCP-SoxS-TetD

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

MCP-SoxS-TetD

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23107

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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lenti_Pemax_t2a_GFP-Kash

Plasmid

#238541

Purpose

Lentiviral expression of Pemax editor with T2A to GFP KASH

Depositor

Jonathan Weissman

Article

Koblan et al Science. 2025 Jul 24:eadx3800. doi: 10.1

Insert

Pemax-T2A-GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pJMP2656

Plasmid

#248701

Purpose

Z. mobilis CRISPRi version 2, promoter C, empty guide

Depositor

Jason Peters

Article

Eckmann et al mSystems. 2025 Dec 4:e0100125. doi: 10.1

Insert

empty sgRNA

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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p305N-24

Plasmid

#246307

Purpose

Evaluation of a synthetic Pol III promoter based on the MtU6.6 promoter for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

MtU6.6m4 promoter

Use

CRISPR

Expression

Plant

Promoter

MtU6.6m4 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-25

Plasmid

#246308

Purpose

Evaluation of a synthetic Pol III promoter based on the MtU6.6 promoter for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

MtU6.6m5 promoter

Use

CRISPR

Expression

Plant

Promoter

MtU6.6m5 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-23

Plasmid

#246306

Purpose

Evaluation of a synthetic Pol III promoter based on the MtU6.6 promoter for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

MtU6.6m1 promoter

Use

CRISPR

Expression

Plant

Promoter

MtU6.6m1 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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